Biological activities of organic compounds adsorbed onto ambient air particles: comparison between the cities of Teplice and Prague during the summer and winter seasons 2000–2001

Binková, Blanka; Černá, Milena; Pastorková, Anna; Jelínek, R; Beneš, I; Jiri, Novak; Šrám, Radìm J.

doi:10.1016/s0027-5107(02)00312-3

Cited by 120 publications

(44 citation statements)

References 35 publications

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“…Comparable values were reported by Nardini [34]; 19 -82 rev/m³ in winter and between 3 -39 rev/m³ in summer. Others like Binkova [35] and Zhoa [36] described lower activies but still with a considerable difference between winter and summer, activities ranged between 13-25 rev/m³ in winter and 4-8 rev/m³ in summer and between 7-24 rev/m³ in winter and 2-8 rev/m³ in summer, respectively. In our study activities in winter were at the same level (4-100 rev/m³) but the activities measured during summer were 2 to 4 times higher then those reported elsewhere.…”

Section: Discussionmentioning

confidence: 98%

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Genotoxic and mutagenic activity of environmental air samples in Flanders, Belgium

Four

Larebeke

Janssen

2004

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Section: Discussionmentioning

confidence: 98%

“…This might be explained by the use of PUF cartridges next to the QF filters in our sampling campaign. Most papers reporting mutagenic activity of ambient air only used filters [33][34][35][36][37][38][39][40]. For the summer samples, 43% of the total activity was found in de PUF extracts whereas only 10% was found in winter.…”

Section: Discussionmentioning

confidence: 99%

Genotoxic and mutagenic activity of environmental air samples in Flanders, Belgium

Four

Larebeke

Janssen

2004

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…Then extraction with DCM was performed in the absence of light for 16 hr plus a 15-min reflux. 17,18 After that, the obtained extract was divided into two portions for chemical analyses and biological assays. Then the extracts were thickened until dry in a vacuum evaporator and finally blown through with nitrogen.…”

Section: Extraction Of the Filtersmentioning

confidence: 99%

Mutagenicity of Airborne Particulates Assessed by Salmonella Assay and the SOS Chromotest in Wrocław, Poland

Piekarska

2010

Journal of the Air & Waste Management Association

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Ambient air particulate matter less than 2.5 m in aerodynamic diameter (PM 2.5 ) samples were collected during summer and autumn using a Staplex high-volume air sampler. They were later extracted with dichloromethane in a Soxhlet apparatus. Polyaromatic hydrocarbon (PAH) content in extracts was determined by the high-performance liquid chromatography technique using fluorescence detection, whereas the nitro-PAH content was determined by gas chromatography using mass detection. Four Salmonella typhimurium strains (TA98, TA100, YG1041, and YG1042) were used in assays conducted with and without metabolic activation. The extracts were also tested with the SOS chromotest supplied by Environmental Biodetection Products Incorporated. The obtained results confirmed the Salmonella assay and the SOS chromotest usability for the purpose of atmospheric pollution monitoring within an urban agglomeration. The atmospheric pollution extracts under examination differed among each other regarding total content and percentage of individual compounds, depending on the season of sampling. The highest total PAH content and the highest nitro-PAH content in the tested samples as well as the most extensive range of detected compounds were found in the autumn season (heating season). The highest mutagenicity was noted for PM 2.5 samples collected in autumn. The high values of mutagenicity ratios and induction factors were obtained from assays carried out with and without metabolic activation, which is an argument for the presence of promutagens and direct mutagens. The YG1041 strain proved to be the most effective in detection of mutagenicity of the suspended dust extracts because of its notably high sensitivity to nitro-aromatic compounds. The SOS chromotest was very sensitive to a large spectrum of genotoxic air pollutants and showed a high degree of similarity with the results of the Salmonella assay. In comparison with the frequently used Ames test, the SOS chromotest enables quick analysis of the genotoxic effects of samples using only one tester strain. In addition, its miniaturized design decreases the consumption of tested samples.

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“…The analysis of PAH-DNA adduct formation by 32P-postlabeling method was performed according to a standard procedure (7,23). DNA was isolated from peripheral lymphocytes, and enriched by nuclease P1.…”

Section: Dna Adductsmentioning

confidence: 99%

“…PAHs are metabolized to form a wide range of products including highly reactive epoxides, which have capacity to bind to DNA, forming PAH-DNA adducts (4,5) and induce oxidative damage (6). Using acellular system Binkova et al (7) observed, that the genotoxicity of respirable particulate matter was related to the content of PAHs. Growing body of evidence suggests that ambient air exposure to B[a]P at levels over 1.0 ng/m 3 induces DNA damage (8).…”

Section: Introductionmentioning

confidence: 99%

Impact of Air Pollution to Genome of Newborns

Šrám

Rössner

Rössnerová

et al. 2016

Cent Eur J Public Health

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SUMMARYThe Northern Moravia Region is the most polluted region in the Czech Republic by particulate matter (PM 2.5 ) and carcinogenic polycyclic aromatic hydrocarbons (c-PAHs) as benzo [a]pyrene (B[a]P) by heavy industry and local heating. This specific situation was used to study the impact of air pollution on newborns in the exposed Karviná district and control district of České Budějovice. Biological material from newborns and mothers was collected in summer and winter seasons. This project is highly detailed, analyzing the concentrations of PAHs in ambient air and diet, in human breast milk, in the urine of mothers and newborns, using biomarkers of genetic damage as DNA adducts and gene expression analysis, biomarkers of oxidative stress as 8-oxodG adducts and lipid peroxidation (15-F2t-isoprostane immunoassay). All 400 children, for whom the biomarker data at delivery were obtained, will be followed for morbidity up to 2 years of age. The Northern Moravia Region seems to be to be a model area for studying the long-term impact of human health exposure to c-PAHs. Our observations will indicate possible genetic and oxidative damage in newborns, which may significantly affect their morbidity.

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Biological activities of organic compounds adsorbed onto ambient air particles: comparison between the cities of Teplice and Prague during the summer and winter seasons 2000–2001

Cited by 120 publications

References 35 publications

Genotoxic and mutagenic activity of environmental air samples in Flanders, Belgium

Genotoxic and mutagenic activity of environmental air samples in Flanders, Belgium

Mutagenicity of Airborne Particulates Assessed by Salmonella Assay and the SOS Chromotest in Wrocław, Poland

Impact of Air Pollution to Genome of Newborns

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