Biological Activities of Novel Zaragozic Acids, the Potent Inhibitors of Squalene Synthase, Produced by the Fungus, Mollisia sp. SANK 10294.

New Metabolites from the Endophytic Fungus Mollisia sp.

“…(Dermateaceae) BCRC 09F0002, was isolated from the root bark of Ardisia cornudentata Mez (Myrsinaceae). Previous chemical investigations of the genus Mollisia have received less attention, and only a few articles have been reported [11][12][13][14][15]. In the course of our search for potentially diverse secondary metabolites from natural fungal sources, and to further understanding the minor metabolites of the genus Mollisia, we examined an EtOAc-soluble fraction of Mollisia sp., which showed inhibitory activity on lipopolysaccharide (LPS)-induced nitric oxide (NO) release and interleukin-6 (IL-6) production in RAW 264.7 murine macrophages, as determined by our primary screening (approximately 90% inhibition at a concentration of 10 Pg/mL).…”

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Two new metabolites, mollisinols A and B (1 and 2), and one first isolated from nature, mollisilactone (3), along with 13 known compounds (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16), were isolated from the EtOAc-soluble fraction of the solid-state fermentation of the endophytic fungus Mollisia sp., derived from the root bark of Ardisia cornudentata Mez (Myrsinaceae). Their structures were elucidated by spectroscopic analyses, including 1D and 2D NMR experiments, and by HR-ESI-MS mass spectrometry.

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“…In addition to the three compounds (1-3) mentioned above, the following 13 known compounds were also isolated: ilanepyrrolal (4) [17], palmitic acid (5) [18], (R)-(-)-mevalolactone (6) [19,20], chrysophanol (7) [21], emodin (8) [22], E-sitostenone (9) [23], a mixture of E-sitosterol (10) and stigmasterol (11) [24], ergosterol peroxide (12) [25], 9,11-dehydroergosterol peroxide (13) [26], 9-hydroxycerevisterol (14) [27], cerevisterol (15) [28], and (22E,24R)-ergosta-8(14),22-diene-3E,5D,6E,7D-tetrol (16) [29].…”

Zaragozic acids production from discomycetes

“…We therefore focused on discomycetes, in particular, on Leotiales, a group of inoperculate discomycetes with smaller apothecia, because they show a wide range of biodiversity and are relatively easily cultivated. As a result, we obtained four ZA analogs, F-10863A, B, C, D, three of which were new compounds (Tanimoto et al, 1997). This paper describes the mycological characteristics of the producing organisms and fermentative production of F-10863s.…”

“…This paper describes the mycological characteristics of the producing organisms and fermentative production of F-10863s. The structure and biological activity of the F-10863s has been described elsewhere (Tanimoto et al, 1997).…”