Biological activities of leaf extracts from selected <i>Kalanchoe</i> species and their relationship with bufadienolides content

Stefanowicz–Hajduk, Justyna; Hering, Anna; Gucwa, Magdalena; Hałasa, Rafał; Soluch, Agata; Kowalczyk, Mariusz; Stochmal, Anna; Ochocka, Renata

doi:10.1080/13880209.2020.1795208

Cited by 23 publications

(17 citation statements)

References 46 publications

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“…Finally, with respect to flavonol glycosides, peak 13 ([M-H]-at m/z 507) was tentatively assigned to spinacetin-O-hexoside taking into account its fragmentation pattern (Boukhris et al, 2016). All these compounds (peaks 3 to 14) belong to the flavonol subclass, which constitutes one of the most abundant flavonoid subclasses previously described for the Bryophyllum subgenus (Fürer et al, 2013;Stefanowicz-Hajduk et al, 2020bTatsimo et al, 2012).…”

Section: Identification and Quantification Of Phenolic Compoundsmentioning

confidence: 97%

Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants

García-Pérez

Ayuso

Lozano-Milo

et al. 2021

Industrial Crops and Products

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Section: Identification and Quantification Of Phenolic Compoundsmentioning

confidence: 97%

Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants

García-Pérez

Ayuso

Lozano-Milo

et al. 2021

Industrial Crops and Products

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“…The cytotoxic activity of bufadienolides in vitro was observed on human leukemia HL-60, lung carcinoma NCI-H460, oral adenosquamous carcinoma Cal-27, lung cancer A549, cervical cancer HeLa, breast cancer MCF-7, ovarian cancer SKOV-3, and melanoma A375 cells [ 20 , 27 , 28 , 29 ]. Additionally, the cytotoxic activity of K. daigremontiana extracts was evaluated on human lymphoblastic leukemia T [ 22 ], human cervical cancer HeLa, ovarian cancer SKOV-3, breast cancer MCF-7, and melanoma A375 cells [ 20 , 30 ]. In our previous study, the water extract of K. daigremontiana compared to the ethanol extract showed significant cytotoxic activity, especially on SKOV-3 cells [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, the cytotoxic activity of K. daigremontiana extracts was evaluated on human lymphoblastic leukemia T [ 22 ], human cervical cancer HeLa, ovarian cancer SKOV-3, breast cancer MCF-7, and melanoma A375 cells [ 20 , 30 ]. In our previous study, the water extract of K. daigremontiana compared to the ethanol extract showed significant cytotoxic activity, especially on SKOV-3 cells [ 30 ]. However, the estimation of cellular factors that play a crucial role in cellular death has not been evaluated.…”

Section: Introductionmentioning

confidence: 99%

An In Vitro Anticancer, Antioxidant, and Phytochemical Study on Water Extract of Kalanchoe daigremontiana Raym.-Hamet and H. Perrier

et al. 2022

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Kalanchoe species are succulents with anti-inflammatory, antioxidant, and analgesic properties, as well as cytotoxic activity. One of the most popular species cultivated in Europe is Kalanchoe daigremontiana Raym.-Hamet and H. Perrier. In our study, we analyzed the phytochemical composition of K. daigremontiana water extract using UHPLC-QTOF-MS and estimated the cytotoxic activity of the extract on human ovarian cancer SKOV-3 cells by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, flow cytometry, luminometric, and fluorescent microscopy techniques. The expression levels of 92 genes associated with cell death were estimated via real-time PCR. The antioxidant activity was assessed via flow cytometry on human keratinocyte HaCaT cell line. The DPPH (2,2-diphenyl-1-picrylhydrazyl) radical and FRAP (ferric-reducing antioxidant power) assays were also applied. We identified twenty bufadienolide compounds in the water extract and quantified eleven. Bersaldegenin-1,3,5-orthoacetate and bryophyllin A were present in the highest amounts (757.4 ± 18.7 and 573.5 ± 27.2 ng/mg dry weight, respectively). The extract showed significant antiproliferative and cytotoxic activity, induced depolarization of the mitochondrial membrane, and significantly arrested cell cycle in the S and G2/M phases of SKOV-3 cells. Caspases-3, 7, 8, and 9 were not activated during the treatment, which indicated non-apoptotic cell death triggered by the extract. Additionally, the extract increased the level of oxidative stress in the cancer cell line. In keratinocytes treated with menadione, the extract moderately reduced the level of oxidative stress. This antioxidant activity was confirmed by the DPPH and FRAP assays, where the obtained IC50 values were 1750 ± 140 and 1271.82 ± 53.25 μg/mL, respectively. The real-time PCR analysis revealed that the extract may induce cell death via TNF receptor (tumor necrosis factor receptor) superfamily members 6 and 10.

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“…Some Kalanchoe taxa are a source of bufadienolides, with cardioactive properties and anticancer activity (for a review, see [28]). A recent study reported that K. blossfeldiana ethanol extract is a potential candidate for cancer and bacterial infection treatment [29]. In contrast, another study shows evidence that K. pinnata, a widely used medicinal plant, may cause DNA damage and its use should be restricted [30].…”

Section: Introductionmentioning

confidence: 99%

The Genus Kalanchoe (Crassulaceae) in Ecuador: From Gardens to the Wild

Vargas

Herrera

Nualart

et al. 2022

Plants

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The genus Kalanchoe, mostly indigenous from Madagascar and Tropical Africa, is widely traded for ornamental value. In this study, we provided an updated list of wild and cultivated Kalanchoe taxa in Ecuador; we analyzed the temporal–spatial pattern of their records, and we categorized the invasion status for each taxon and its environment preferences. The records of any taxa belonging to this genus were compiled from an extensive search using various information sources. Our results confirmed the presence of 16 taxa of Kalanchoe in the country. Seven species and a hybrid were detected in the wild. Kalanchoe densiflora, K. laxiflora, K. pinnata, K. tubiflora, and K. ×houghtonii were categorized as invasive. We detected invasive records of some of these plants in protected areas. Almost all taxa had at least one record as cultivated, suggesting that the invasion pathway is ornamental trade. Kalanchoe pinnata individuals in the wild were recorded in the four biogeographic regions of Ecuador, which could be associated with the wide range of precipitations and temperatures in which the species may dwell. Our study highlights the importance of reducing the ornamental value and limiting the use of Kalanchoe taxa with invasive potential in horticulture and promoting, instead, the use of indigenous species.

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Biological activities of leaf extracts from selected Kalanchoe species and their relationship with bufadienolides content

Cited by 23 publications

References 46 publications

Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants

Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants

An In Vitro Anticancer, Antioxidant, and Phytochemical Study on Water Extract of Kalanchoe daigremontiana Raym.-Hamet and H. Perrier

The Genus Kalanchoe (Crassulaceae) in Ecuador: From Gardens to the Wild

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