Biological Activities of Anti-Merozoite Surface Protein-1 Antibodies Induced by Adjuvant-Assisted Immunizations in Mice with Different Immune Gene Knockouts

Hui, George; Choe, Dan; Hashimoto, Caryn

doi:10.1128/cvi.00058-08

Cited by 7 publications

(12 citation statements)

References 45 publications

(64 reference statements)

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“…The mean titer induced by rMSP1-QDs was two to three logs higher than those induced by CFA and ISA51. Moreover, these levels of antibodies were much higher than levels with any other adjuvants we have tested in previous studies with MSP1 vaccines [47, 54]. Results from antibody subclass determination and ELISPOTs showed that QD immunizations potentiated a balanced TH1/TH2 response.…”

Section: Discussionmentioning

confidence: 76%

“…Purified mouse antibodies from all immunized groups were tested for their ability to inhibit parasite growth in vitro [47]. As shown in Table 2, the anti-MSP1-42 antibodies obtained from immunizations with rMSP1-QDs via the i.p., i.m., or s.c. route significantly inhibited parasite growth, with inhibition ranging from 73–81% (Table 2).…”

Section: Resultsmentioning

confidence: 99%

“…As shown in Table 2, the anti-MSP1-42 antibodies obtained from immunizations with rMSP1-QDs via the i.p., i.m., or s.c. route significantly inhibited parasite growth, with inhibition ranging from 73–81% (Table 2). None of the anti-MSP1-42 antibodies induced by rMSP1-CFA and rMSP1-ISA51 inhibited parasite growth by more than 50%, a level that is considered to be biologically significant [47, 53]. …”

Section: Resultsmentioning

confidence: 99%

“…The immunoglobulin isotypes of the anti-MSP1-19 specific antibodies were determined by isotype specific ELISAs as previously described [47]. Goat anti-mouse-IgG1 and IgG2a (Southern Biotechnology, Birmingham, AL) were used at a dilution of 1:4000.…”

Section: Methodsmentioning

confidence: 99%

“…Immunoglobulins from pooled mouse serum samples from each group were purified as previously described [47] with modifications. Briefly, antibodies were purified by ammonium sulfate precipitation and followed by dialysis using an Amicon Ultra-10 Centrifugal Filter (Millipore, Billerica, MA) with a molecular weight cut off of 100 kDa.…”

Section: Methodsmentioning

confidence: 99%

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Blood stage merozoite surface protein conjugated to nanoparticles induce potent parasite inhibitory antibodies

Pusic

Stridiron

et al. 2011

Vaccine

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In this proof-of-concept study we report the use of <15nm, water soluble, inorganic nanoparticles as a vaccine delivery system for a blood stage malaria vaccine. The recombinant malarial antigen, Merozoite Surface Protein 1 (rMSP1) of P. falciparum served as the model vaccine. The rMSP1 was covalently conjugated to polymer-coated quantum dot CdSe/ZnS nanoparticles (QDs) via surface carboxyl groups, forming rMSP1-QDs. Anti-MSP1 antibody responses induced by rMSP1-QDs were found to have 2 to 3 log higher titers than those obtained with rMSP1 administered with the conventional adjuvants, Montanide ISA51 and CFA. Moreover, the immune responsiveness and the induction of parasite inhibitory antibodies were significantly superior in mice injected with rMSP1-QDs. The rMSP1-QDs delivered via intra-peritoneal (i.p.), intra-muscular (i.m.), and subcutaneous (s.c.) routes were equally efficacious. The high level of immunogenicity exhibited by the rMSP1-QDs was achieved without further addition of other adjuvant components. Bone marrow derived dendritic cells were shown to efficiently take up the nanoparticles leading to their activation and the expression/secretion of key cytokines, suggesting that this may be a mode of action for the enhanced immunogenicity. This study provides promising results for the use of water soluble, inorganic nanoparticles (<15nm) as potent vehicles/platforms to enhance the immunogenicity of polypeptide antigens in adjuvant-free immunizations.

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Section: Discussionmentioning

confidence: 76%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Blood stage merozoite surface protein conjugated to nanoparticles induce potent parasite inhibitory antibodies

Pusic

Stridiron

et al. 2011

Vaccine

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Poly(I:C) adjuvant strongly enhances parasite-inhibitory antibodies and Th1 response against Plasmodium falciparum merozoite surface protein-1 (42-kDa fragment) in BALB/c mice

Mehrizi

Rezvani

Zakeri

et al. 2018

Med Microbiol Immunol

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Malaria vaccine development has been confronted with various challenges such as poor immunogenicity of malaria vaccine candidate antigens, which is considered as the main challenge. However, this problem can be managed using appropriate formulations of antigens and adjuvants. Poly(I:C) is a potent Th1 inducer and a human compatible adjuvant capable of stimulating both B- and T-cell immunity. Plasmodium falciparum merozoite surface protein 1 (PfMSP-1) is a promising vaccine candidate for blood stage of malaria that has faced several difficulties in clinical trials, mainly due to improper adjuvants. Therefore, in the current study, poly(I:C), as a potent Th1 inducer adjuvant, was evaluated to improve the immunogenicity of recombinant PfMSP-1, when compared to CFA/IFA, as reference adjuvant. Poly(I:C) produced high level and titers of anti-PfMSP-1 IgG antibodies in which was comparable to CFA/IFA adjuvant. In addition, PfMSP-1 formulated with poly(I:C) elicited a higher ratio of IFN-γ/IL-4 (23.9) and IgG2a/IgG1 (3.77) with more persistent, higher avidity, and titer of IgG2a relative to CFA/IFA, indicating a potent Th1 immune response. Poly(I:C) could also help to induce anti-PfMSP-1 antibodies with higher growth-inhibitory activity than CFA/IFA. Altogether, the results of the current study demonstrated that poly(I:C) is a potent adjuvant that can be appropriate for being used in PfMSP-1-based vaccine formulations.

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Antimalarial Activity of Physalins B, D, F, and G

et al. 2011

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The antimalarial activities of physalins B, D, F, and G (1-4), isolated from Physalis angulata, were investigated. In silico analysis using the similarity ensemble approach (SEA) database predicted the antimalarial activity of each of these compounds, which were shown using an in vitro assay against Plasmodium falciparum. However, treatment of P. berghei-infected mice with 3 increased parasitemia levels and mortality, whereas treatment with 2 was protective, causing a parasitemia reduction and a delay in mortality in P. berghei-infected mice. The exacerbation of in vivo infection by treatment with 3 is probably due to its potent immunosuppressive activity, which is not evident for 2.

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Biological Activities of Anti-Merozoite Surface Protein-1 Antibodies Induced by Adjuvant-Assisted Immunizations in Mice with Different Immune Gene Knockouts

Abstract: Immunizations with

Cited by 7 publications

References 45 publications

Blood stage merozoite surface protein conjugated to nanoparticles induce potent parasite inhibitory antibodies

Blood stage merozoite surface protein conjugated to nanoparticles induce potent parasite inhibitory antibodies

Poly(I:C) adjuvant strongly enhances parasite-inhibitory antibodies and Th1 response against Plasmodium falciparum merozoite surface protein-1 (42-kDa fragment) in BALB/c mice

Antimalarial Activity of Physalins B, D, F, and G

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