Biologia de Anofelinos Amazônicos. VI. Variabilidade Enzimática em ANOPHELES DARLINGI Root. (DIPTERA, CULICIDAE)

Contel, E. P. B.; Santos, Joselita Maria Mendes dos; Tadei, Wanderli Pedro

doi:10.1590/1809-43921984142243

Cited by 3 publications

(3 citation statements)

References 5 publications

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“…Low variation was found in A. darlingi for this enzyme, in agreement with data in the literature for the majority of Anopheles species of the subgenus Nyssorhynchus (NARANG et ai, 1979a(NARANG et ai, , 1979bSTEINER et ai, 1980;CONTEL, et al, 1984; …”

Section: Leucine Aminopeptidasesupporting

confidence: 88%

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Electrophoretic Analysis of 11 Enzymes in Natural Populations Ofroot, 1926 (Diptera: Culicidae) in the Amazon Region

1996

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-Of eleven proteins analyzed in four Amazonian populations, the esterases showed the greatest variation, with five activity zones. EST1, EST2 and EST5 showed variation in each of the populations studied. EST1 and EST2 are each controlled by two, and EST5 by four, codominant alleles. LAP presented six activity zones, with codominant variation in LAP5 and LAP6. oc-GPDH was monomorphic with one activity band on starch gel and two on polyacrylamide gel. 1DH presented two activity zones, with variation in the IDHl region. PGM had a single activity zone, with variation in all populations. The Ariquemes populations showed five alleles and the other populations three, all of then codominant. Three activity zones with two codominant alleles were observed for ODH. Aldehyde Oxidase showed two activity zones, with variation in AOl only in the Ariquemes and Porto Velho/Samuel populations. 6-PGDH showed only one activity zone and variation only in the Ariquemes population. The remaing systems -XDH, G-6-PDH and GDH. was monomorphic. Key words: Anopheles darlingi, isozymes, electrophoresisAnálise Eletroforética de 11 Enzimas era Populações Naturais de Anopheles (Ν.) darlingi Root, 1926 (Diptera: Cuiicidae) na Região Amazônica.RESUMO -Dos 11 sistemas protéicos analisados, em quatro populações da Amazônia, as esterases foram as que apresentaram maior variação, com cinco zonas de atividade. EST1, EST2, e EST5 mostraram variação genética determinada em todas populações estudadas, sendo que as duas primeiras são resultantes do controle de dois alelos e a EST5 de quatro, todos codominantes. A LAP consiste de seis zonas de atividade e com variação apenas nos loci LAP5 e LAP6, ambos codominantes. A GPDH mostrou-se monomórfica com uma zona de atividade em gel de amido e duas em poliacrilamida. A IDH apresentou duas zonas de atividade, com variação na região da IDH1. A PGM consiste de uma única zona de atividade e com variação em todas as populações estudadas. A população de Ariquemes revelou cinco alelos e as demais três, todos codominantes. Para a ODH, foram observadas três zonas de atividade com dois alelos codominantes. A enzima AO apresentou duas zonas de atividade, sendo que a AOl mostrou-se variável apenas em Ariquemes e Porto Velho/Samuel. 6-PGDH possui apenas uma zona de atividade e variação apenas em Ariquemes. Os demais sistemas -XDH, G-6-PDH e GDH -mostraram-se monomórficos.

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Section: Leucine Aminopeptidasesupporting

confidence: 88%

“…For Ariquemes (RO) and High way PA-422 (PA) populations, CONTEL et al (1984) reported that the three loci detected were monomor phic in two populations. In a popula tion of A. nuneztovari from Tucurui (PA), SCARPASSA et al (1992) de tected variability only for LAP5.…”

Section: Scarpassa Et Al 1992)mentioning

confidence: 99%

Electrophoretic Analysis of 11 Enzymes in Natural Populations Ofroot, 1926 (Diptera: Culicidae) in the Amazon Region

1996

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“…25 In addition, variation has been described in its morphology, 26,27 chromosomes, 28,29 and isozymes. 18,[30][31][32] Some of these reports have suggested that An. darlingi may be a species complex.…”

Section: Anopheles (Nyssorhynchus) Darlingimentioning

confidence: 99%

Population structure of the primary malaria vector in South America, Anopheles darlingi, using isozyme, random amplified polymorphic DNA, internal transcribed spacer 2, and morphologic markers.

Manguin

Wilkerson²,

Conn³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

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Abstract. A genetic and morphologic survey of Anopheles darlingi populations collected from seven countries in Central and South America was performed to clarify the taxonomic status of this major malaria vector species in the Americas. Population genetics was based on three techniques including isozyme, random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR), and internal transcribed spacer 2 (ITS2) markers. The results of the isozyme analysis indicated moderate differences in the allele frequencies of three putative loci (glutamate oxalaoacetate transaminase-1, isocitrate dehydrogenase-1, and phosphoglucomutase) of the 31 analyzed. No fixed electromorphic differences separated the populations of An. darlingi, which showed little genetic divergence (Nei distances ϭ 0.976-0.995). Fragments produced by RAPD-PCR demonstrated evidence of geographic partitioning and showed that all populations were separated by small genetic distances as measured with the 1 Ϫ S distance matrix. The ITS2 sequences for all samples were identical except for four individuals from Belize that differed by a three-base deletion (CCC). The morphologic study demonstrated that the Euclidean distances ranged from 0.02 to 0.14, with the highest value observed between populations from Belize and Bolivia. Based on these analyses, all the An. darlingi populations examined demonstrated a genetic similarity that is consistent with the existence of a single species and suggest that gene flow is occurring throughout the species' geographic range.

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Biologia De Anofelinos Amazônicos. Xv. Leucina Aminopeptidase Em Anopheles (Nyssorhynchus) Nuñez-Tovari; Ontogenia E Variação Genética

Scarpassa

Tadei

Contel³

1992

Acta Amaz.

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RESUMOPados sobre a leucina aminopeptidase durante o desenvolvimento ontogenético em Anopheles nuñez-tovarì evidenciaram seis zonae de atividade, eendo a LAP1, LAP2, LAP4 e LAP5 presentes nos estádios larvais, pupae e adultos e a LAP3 e LAP6 características desses dois últimos estágios. Diferenças na intensidade de coloração foram detectadas conforme o estágio considerado. A LAP1 e LAP5 apresentam intensidade fraca em todos os estágios e a LAP2 e LAP4 mostram atividade intensa nos estádios larvais, diminuindo nos dois estágios subseqüentes. Considerando-se as funções das enzimas, foi admitido que a LAP3, no estágio de pupa, possa estar relacionada com a histólise dos tecidos larvais. Dos seis loci detectados, variação alélica foi constatada apenas para o locus LAP5, sendo detectados dois alelos de ação codominante. As freqüências genotípicas de progênies de fêmeas inseminadas naturalmente desviam-se significativamente do equilíbrio de Hardy-Weinberg.

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Biologia de Anofelinos Amazônicos. VI. Variabilidade Enzimática em ANOPHELES DARLINGI Root. (DIPTERA, CULICIDAE)

Cited by 3 publications

References 5 publications

Electrophoretic Analysis of 11 Enzymes in Natural Populations Ofroot, 1926 (Diptera: Culicidae) in the Amazon Region

Electrophoretic Analysis of 11 Enzymes in Natural Populations Ofroot, 1926 (Diptera: Culicidae) in the Amazon Region

Population structure of the primary malaria vector in South America, Anopheles darlingi, using isozyme, random amplified polymorphic DNA, internal transcribed spacer 2, and morphologic markers.

Biologia De Anofelinos Amazônicos. Xv. Leucina Aminopeptidase Em Anopheles (Nyssorhynchus) Nuñez-Tovari; Ontogenia E Variação Genética

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