Biolistic transfer and expression of a uidA reporter gene in different tissues of Allium sativum L.

Transient Expression of Green Fluorescent Protein in Rice Calluses : Optimization of Parameters for Helios Gene Gun Device

“…It appears that the sgfp expression was genotype-dependent in terms of differences in callus cell types they produced, which has been described by Bec et al (1998). Moreover, differences in the sgfp expression in calluses of the two cultivars bombarded using a Helios gene gun might be attributed to the difference in biological constraint such as the activity of endogenous nuclease (Barandiaran et al, 1998), local RNA silencing which blocks expression of transgene (Baulcombe, 2004;Miki et al, 2005) and physical constraint such as callus cell density, callus compactness or callus friability that might not be exactly identical in these cultivars.…”

Efficient plant regeneration of Malaysian aromatic rice (Oryza sativa L.) via improved somatic embryogenesis pathway

“…A similar phenomena was reported by Thengane et al (2006) with addition of NAA (5.3-10.7 µM) which gave rise to germinating embryos with complete well-developed shoots. In garlic, low level of 2,4-D increased the percentage of explants producing callus and subsequently tend to give rise to fine compact callus formation (Barandiaran, 1999).…”

Evidence of a somatic embryogenesis process for plant regeneration in garlic ( Allium sativum L.)

“…Callus culture and plant regeneration have been proved feasible from various parts of the plant: shoot-tips (Kehr and Schaeffer 1976;Abo El-Nil 1977;Novak 1981;Nagakubo et al 1993;Koul et al 1994;Myers and Simon 1998), basal plates (Xue et al 1991;Koch et al 1995;Barandiaran et al 1998), first primordial leaves (Novak 1980;Dolezel and Novak 1985;Sata et al 2001) and root-tips (Haque et al 1997(Haque et al , 1998Barandiaran et al 1999a, b;RobledoPaz et al 2000). However, somatic embryogenesis was not proved in these papers and uncontrolled somaclonal variation has been reported (Al-Zahim et al 1999).…”