Biokinetics and clearance of inhaled gold ultrasmall-in-nano architectures

Mapanao, Ana Katrina; Giannone, Giulia; Summa, Maria; Ermini, Maria Laura; Zamborlin, Agata; Santi, Melissa; Cassano, Domenico; Bertorelli, Rosalia; Voliani, Valerio

doi:10.1039/d0na00521e

Cited by 26 publications

(51 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of USNPs within NAs was shown previously [ 47 ]. Here, the differences in the TEM and DLS measurement confirm the successful decoration of NAs with HSA without affecting the structure of the final nano-architectures.…”

Section: Resultssupporting

confidence: 58%

“…We observed a very promising biosafety profile in zebrafish models [ 55 ], and confirmed these data further in healthy murine models [ 38 ]. Furthermore, we investigated the biokinetics of standard Nas in animal models and confirmed that the building blocks are metabolized or excreted after biodegradation [ 47 ]. Finally, it is important to highlight that the hydrodynamic diameter of Nas is not significantly altered over 8 h incubation in blood-mimicking solutions [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…Further studies are needed to understand how the unique and complex extracellular environment of the brain tumours may influence the penetration and drug release of these nanoparticles. It would also be worth noting that other administration pathways, such as intranasal delivery, may result in an improved effect due to the enhanced transient accumulation of NAs in the brain [ 47 ]. Additionally, the employment of focused ultrasound together with chemotherapy-loaded liposomes has shown some promise in glioblastoma bearing mice [ 72 , 73 ].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Doxorubicin-Loaded Gold Nanoarchitectures as a Therapeutic Strategy against Diffuse Intrinsic Pontine Glioma

Ung

Tsoli

Liu

et al. 2021

Cancers

Self Cite

View full text Add to dashboard Cite

Diffuse Intrinsic Pontine Gliomas (DIPGs) are highly aggressive paediatric brain tumours. Currently, irradiation is the only standard treatment, but is palliative in nature and most patients die within 12 months of diagnosis. Novel therapeutic approaches are urgently needed for the treatment of this devastating disease. We have developed non-persistent gold nano-architectures (NAs) functionalised with human serum albumin (HSA) for the delivery of doxorubicin. Doxorubicin has been previously reported to be cytotoxic in DIPG cells. In this study, we have preclinically evaluated the cytotoxic efficacy of doxorubicin delivered through gold nanoarchitectures (NAs-HSA-Dox). We found that DIPG neurospheres were equally sensitive to doxorubicin and doxorubicin-loaded NAs. Colony formation assays demonstrated greater potency of NAs-HSA-Dox on colony formation compared to doxorubicin. Western blot analysis indicated increased apoptotic markers cleaved Parp, cleaved caspase 3 and phosphorylated H2AX in NAs-HSA-Dox treated DIPG neurospheres. Live cell content and confocal imaging demonstrated significantly higher uptake of NAs-HSA-Dox into DIPG neurospheres compared to doxorubicin alone. Despite the potency of the NAs in vitro, treatment of an orthotopic model of DIPG showed no antitumour effect. This disparate outcome may be due to the integrity of the blood-brain barrier and highlights the need to develop therapies to enhance penetration of drugs into DIPG.

show abstract

Section: Resultssupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Doxorubicin-Loaded Gold Nanoarchitectures as a Therapeutic Strategy against Diffuse Intrinsic Pontine Glioma

Ung

Tsoli

Liu

et al. 2021

Cancers

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is important to highlight here that there is only one site where the nervous system of mammals is directly exposed to the external environment. This is the roof of the nasal cavity which represents the intranasal route and has been investigated for nanoparticle entry into the brain bypassing the BBB [ 49 ]. Direct uptake of nanoparticles into the nervous system might occur in this site thus it can be another viable administration route that deserves to be explored.…”

Section: Discussionmentioning

confidence: 99%

Iron Oxide Incorporated Conjugated Polymer Nanoparticles for Simultaneous Use in Magnetic Resonance and Fluorescent Imaging of Brain Tumors

et al. 2021

View full text Add to dashboard Cite

Conjugated polymer nanoparticles (CPNs) have emerged as advanced polymeric nanoplatforms in biomedical applications by virtue of extraordinary properties including high fluorescence brightness, large absorption coefficients of one and two-photons, and excellent photostability and colloidal stability in water and physiological medium. In addition, low cytotoxicity, easy functionalization, and the ability to modify CPN photochemical properties by the incorporation of dopants, convert them into excellent theranostic agents with multifunctionality for imaging and treatment. In this work, CPNs were designed and synthesized by incorporating a metal oxide magnetic core (Fe3O4 and NiFe2O4 nanoparticles, 5 nm) into their matrix during the nanoprecipitation method. This modification allowed the in vivo monitoring of nanoparticles in animal models using magnetic resonance imaging (MRI) and intravital fluorescence, techniques widely used for intracranial tumors evaluation. The modified CPNs were assessed in vivo in glioblastoma (GBM) bearing mice, both heterotopic and orthotopic developed models. Biodistribution studies were performed with MRI acquisitions and fluorescence images up to 24 h after the i.v. nanoparticles administration. The resulting IONP-doped CPNs were biocompatible in GBM tumor cells in vitro with an excellent cell incorporation depending on nanoparticle concentration exposure. IONP-doped CPNs were detected in tumor and excretory organs of the heterotopic GBM model after i.v. and i.t. injection. However, in the orthotopic GBM model, the size of the nanoparticles is probably hindering a higher effect on intratumorally T2-weighted images (T2WI) signals and T2 values. The photodynamic therapy (PDT)—cytotoxicity of CPNs was not either affected by the IONPs incorporation into the nanoparticles.

show abstract

“…The ultrasmall gold nanoparticles could be completely eliminated from other secondary organs within 10 d. This study lays the foundation for the use of metal in the treatment of lung tumors, asthma, and other respiratory diseases. [ 146 ]…”

Section: Factors Affecting Renal Clearancementioning

confidence: 99%

Recent Advances in Renal Clearable Inorganic Nanoparticles for Cancer Diagnosis

Yin

Zhang

et al. 2020

Part & Part Syst Charact

View full text Add to dashboard Cite

show abstract

Biokinetics and clearance of inhaled gold ultrasmall-in-nano architectures

Abstract: Among the organism’s entry portals, the respiratory tract is one of the most promising routes for a non-invasive administration of therapeutics for local and systemic delivery. On the other hand,...

Cited by 26 publications

References 28 publications

Doxorubicin-Loaded Gold Nanoarchitectures as a Therapeutic Strategy against Diffuse Intrinsic Pontine Glioma

Doxorubicin-Loaded Gold Nanoarchitectures as a Therapeutic Strategy against Diffuse Intrinsic Pontine Glioma

Iron Oxide Incorporated Conjugated Polymer Nanoparticles for Simultaneous Use in Magnetic Resonance and Fluorescent Imaging of Brain Tumors

Recent Advances in Renal Clearable Inorganic Nanoparticles for Cancer Diagnosis

Contact Info

Product

Resources

About