Bioinspired Structure Regulation of Apyrase-Like Nanozyme with Intracellular-Generated H₂O₂ for Tumor Catalytic Therapy

Abstract

Adenosine triphosphate (ATP) is the major resource of energy supply in tumor activity. Therefore, improving ATP consumption efficiencies is a promising approach for cancer therapy. Herein, inspired by the H2O2-involved structure regulation effect during the catalysis of natural protein enzymes, we developed an artificial H2O2-driven ATP catalysis-promoting system, the Ce-based metal–organic framework (Ce-MOF), for catalytic cancer therapy. In the presence of H2O2, the hydrolysis ATP activity of Ce-MOF­(H2O2) was enhanced by around 1.6 times. Taking advantage of the endogenous H2O2 in cancerous cells, catalytic hydrolysis for intracellular ATP of the Ce-MOF achieves the inhibition of cancerous cell growth, which involves damaged mitochondrial function and autophagy-associated cell death. Furthermore, in vivo studies suggest that the Ce-MOF has a good tumor inhibition effect. The artificial H2O2-driven ATP catalysis-promoting system not only demonstrates high catalytic ATP consumption efficiencies for cancer therapy but also highlights a bioinspired strategy to expedite nanozyme research in both design and applied sciences.

Tunable metal–organic frameworks assist in catalyzing DNAzymes with amplification platforms for biomedical applications

“…Due to the presence of Ce 4+ /Ce 3+ ion pairs and sites in cerium (quadrivalent) MOFs, the outstanding oxidation–reduction reaction (REDOX) and photocatalytic activity of cerium (quadrivalent) MOFs exhibited a wide range of applications. 57 Au nanoparticles could accurately convert the original Ce 3+ node to Ce 4+ . With the stable existence of Ce 4+ , the coordination of Ce nodes changes, resulting in an irregular structure and an obvious obstruction of electron–hole recombination, which promotes reaction generation.…”