Bioinspired protein microparticles fabrication by peptide mediated disulfide interchange

Lai, Kwok Kei; Renneberg, Reinhard; Mak, Wing Cheung

doi:10.1039/c4ra00102h

Cited by 8 publications

(5 citation statements)

References 39 publications

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“…The biological module (BSA-GOxMP) and transducer module (POM–PPyMP) were prepared using a generic CaCO 3 template-assisted technique. The porous structure and inorganic nature of the CaCO 3 allows the effective loading of various small organic, inorganic, and biological molecules such as monomer (i.e., pyrrole), electrocatalyst–Dawson-type polyoxometalate (POM), or enzyme (e.g., glucose oxidase GOx), thus providing a universal method for fabrication of microparticles with different chemical natures. − The macroscopic morphologies of the BSA-GOxMPs and POM–PPyMPs were characterized by optical microscopy. Optical images showed that the BSA-GOxMPs and POM–PPyMPs exhibited a fairly uniform spherical shape and a homogeneous narrow size distribution (Figure A,B) with an average diameter of 2.3 ± 0.3 μm and 2.6 ± 0.4 μm, respectively (Figure S1).…”

Section: Resultsmentioning

confidence: 99%

Printable Heterostructured Bioelectronic Interfaces with Enhanced Electrode Reaction Kinetics by Intermicroparticle Network

Wannapob

Vagin

Liu

et al. 2017

ACS Appl. Mater. Interfaces

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Printable organic bioelectronics provide a fast and cost-effective approach for the fabrication of novel biodevices, while the general challenge is to achieve optimized reaction kinetics at multiphase boundaries between biomolecules and electrodes. Here, we present an entirely new concept based on a modular approach for the construction of heterostructured bioelectronic interfaces by using tailored functional "biological microparticles" combined with "transducer microparticles" as modular building blocks. This approach offers high versatility for the design and fabrication of bioelectrodes with a variety of forms of interparticle spatial organization, from layered-structures to more advance bulk heterostructured architectures. The heterostructured biocatalytic electrodes delivered twice the reaction rate and a six-fold increase in the effective diffusion kinetics in response to a catalytic model using glucose as the substrate, together with the advantage of shortened diffusion paths for reactants between multiple interparticle junctions and large active particle surface. The consequent benefits of this improved performance combined with the simple means of mass production are of major significance for the emerging printed electronics industry.

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Section: Resultsmentioning

confidence: 99%

Printable Heterostructured Bioelectronic Interfaces with Enhanced Electrode Reaction Kinetics by Intermicroparticle Network

Wannapob

Vagin

Liu

et al. 2017

ACS Appl. Mater. Interfaces

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“…99 Aggregation was enabled by the specific affinity between a protein on the beads and the outer lipid bilayer of RBCs following eryptosis, which was instigated either by increasing osmolarity or using a potential anti-cancer drug. Lai et al 104 used TRPS size distributions to observe binding of antibody-loaded protein microparticles to a target molecule. Protein microparticles were suspended in the fluid cell above a pore, and the mean particle diameter increased from 1041.5 nm to 1212.6 nm when the target (mouse IgG) was introduced.…”

Section: Diagnostics and Genomicsmentioning

confidence: 99%

Applications of tunable resistive pulse sensing

Weatherall

Willmott

2015

Analyst

120

121

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Tunable resistive pulse sensing (TRPS) is an experimental technique that has been used to study and characterise colloidal particles ranging from approximately 50 nm in diameter up to the size of cells. The primary aim of this Review is to provide a guide to the characteristics and roles of TRPS in recent applied research. Relevant studies reflect both the maturation of the technique and the growing importance of submicron colloids in fields such as nanomedicine and biotechnology. TRPS analysis of extracellular vesicles is expanding particularly swiftly, while TRPS studies also extend to on-bead assays using DNA and aptamers, drug delivery particles, viruses and bacteria, food and beverages, and superparamagnetic beads. General protocols for TRPS measurement of particle size, concentration and charge have been developed, and a summary of TRPS technology and associated analysis techniques is included in this Review.

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“…In addition to those approaches, the application of a calcium carbonate (CaCO 3 ) template for preparing protein MPs has been reported. For example, one fabrication method used coprecipitation to entrap protein molecules such as BSA inside spherical CaCO 3 templates [ 17 ]. This concept relied on the intracellular disulfide interchange reactions at physiological conditions mediated by glutathione to initiate disulfide interchange reactions between the BSA molecules.…”

Section: Introductionmentioning

confidence: 99%

Glucose Oxidase/Egg White Protein Microparticles with a Redox Mediator for Glucose Biosensors on a Screen-Printed Electrode and a Decomposable Electrode

Rasitanon,

Rattanapan,

Kaewpradub

et al. 2023

Biosensors

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Glucose oxidase (GOx) is a typical model enzyme used to create biosensors. Exploring a strategy to prepare ready-to-use functional enzymatic microparticles combining GOx and food-based proteins offers compelling advantages. However, no reports exist on the integration of egg white materials to synthesize functional biorecognition particles with glucose oxidation catalytic functions for electrochemical biosensors. Here, we demonstrate functional microparticles combining egg white proteins, GOx, and 9,10-phenanthrenequinone (PQ). The egg white proteins crosslink to form three-dimensional scaffolds to accommodate GOx and redox molecules. The PQ mediator enhances electron transfer between the electrode surface and the GOx enzyme’s flavin adenine dinucleotides. The functional microparticles are directly applied to the printed electrode. The performance of these microparticles is evaluated using a screen-printed carbon nanotube (CNT)-modified electrode coated with GOx/PQ/egg white protein microparticles. The analytical performance of the system exhibits a linear range of 0.125−40 mM, with a maximum current (Imax) and a Michaelis–Menten constant (Km) being 0.2 µA and 4.6 mM, respectively. Additionally, a decomposable electrode composed of CNTs and edible oil conjugated with functional enzyme microparticles is shown to undergo degradation under gastric conditions. Utilizing food-based proteins to accommodate enzymes and to create redox-active microparticles for catalyzing glucose oxidation offers advantages in developing affordable and degradable bioelectrodes. This concept holds promise for advancing biocompatible electrodes in biosensor and bioelectronics applications.

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Bioinspired protein microparticles fabrication by peptide mediated disulfide interchange

Abstract: A bioinspired green chemistry approach for the fabrication of pure protein microparticles based on peptide mediated disulfide interchange reactions.

Cited by 8 publications

References 39 publications

Printable Heterostructured Bioelectronic Interfaces with Enhanced Electrode Reaction Kinetics by Intermicroparticle Network

Printable Heterostructured Bioelectronic Interfaces with Enhanced Electrode Reaction Kinetics by Intermicroparticle Network

Applications of tunable resistive pulse sensing

Glucose Oxidase/Egg White Protein Microparticles with a Redox Mediator for Glucose Biosensors on a Screen-Printed Electrode and a Decomposable Electrode

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