Bioinspired Design of Sericin/Chitosan/Ag@MOF/GO Hydrogels for Efficiently Combating Resistant Bacteria, Rapid Hemostasis, and Wound Healing

Abstract

Due to the spread of drug-resistant bacteria in hospitals, the development of antibacterial dressings has become a strategy to control wound infections caused by bacteria. Here, we reported a green strategy for in situ biomimetic syntheses of silver nanoparticles@organic frameworks/graphene oxide (Ag@MOF–GO) in sericin/chitosan/polyvinyl alcohol hydrogel. Ag@MOF–GO was synthesized in situ from the redox properties of tyrosine residues in silk sericin without additional chemicals, similar to a biomineralization process. The sericin/chitosan/Ag@MOF–GO dressing possessed a high porosity, good water retention, and a swelling ratio. The hemolysis rate of the composite was 3.9% and the cell viability rate was 131.2%, which indicated the hydrogel possessed good biocompatibility. The composite also showed excellent lasting antibacterial properties against drug-sensitive and drug-resistant pathogenic bacteria. The composite possessed excellent hemostatic activity. The coagulation effect of the composite may be related to its effect on the red blood cells and platelets, but it has nothing to do with the activation of coagulation factors. An in vitro cell migration assay confirmed and an in vivo evaluation of mice indicated that the composite could accelerate wound healing and re-epithelialization. In summary, the composite material is an ideal dressing for accelerating hemostasis, preventing bacterial infection, and promoting wound healing.

Bioinspired Hydrogels as Platforms for Life-Science Applications: Challenges and Opportunities

“…There is a variety of strategies to develop materials with properties attractive in many biomedical applications. Thus, the hydrogel strength can be tuned from their formulation, from very soft to very hard structure networks, suitable for the targeted applications: from injectable hydrogels [26,34,35] for tissue filling and restoration [3,[36][37][38], biomaterials for rapid hemostasis [39], wound healing [39,40], and drug delivery carriers [35,41] to robust and tough hydrogels required for load-bearing applications (artificial cartilage, muscle) [34] and smart biomaterials for tissue engineering [4,24,42] or advanced technological applications [14,43,44].…”

“…Macromolecules of different structures and architectures can be synthesized using a diversity of available monomers. By mimicking the nature at different levels, various hydrogel structures can be now developed focusing on the targeted properties, such as tunable mechanical properties or stimuli-responsiveness, variable porosity, or improved interactions with cell [3,[10][11][12]39,42,44,47,55,56]. Thus, the research directions concerning the hydrogels are extended to diverse application fields as well.…”

Advances in the Application of Nanomaterials as Treatments for Bacterial Infectious Diseases

“…The efficacy of combined neomycin, an antibiotic with Ag-NPs in bacteria-infected mice with burn wounds revealed good healing ability with excellent wound contraction when the neomycin-Ag-NPs were used in the spray method [107]. Ag NPs@organic frameworks/graphene oxide in sericin/chitosan/polyvinyl alcohol hydrogel were constructed for the redox ability of tyrosine residues in silk sericin without extra chemicals [108]. The composite material was noted to be an ideal dressing for accelerating hemostasis, preventing multi-drug resistant bacterial infection and promoting wound healing [108].…”

“…Ag NPs@organic frameworks/graphene oxide in sericin/chitosan/polyvinyl alcohol hydrogel were constructed for the redox ability of tyrosine residues in silk sericin without extra chemicals [108]. The composite material was noted to be an ideal dressing for accelerating hemostasis, preventing multi-drug resistant bacterial infection and promoting wound healing [108]. Based on the good antibacterial ability of Ag NPs combating numerous bacterial strains compared with other antimicrobial agents, the IPM@AgNPs-PEG-NOTA nanocomposite (Ag NPs covered with SH-PEG-NOTA and loaded by imipenem) was developed [109].…”

Biomimetic Antibacterial Pro-Osteogenic Cu-Sericin MOFs for Osteomyelitis Treatment

“…Silk protein sericin (SER) possesses antibacterial activity [ 10 ]. Diverse metal ions have successfully been complexed with SER to further improve the antibacterial properties of the resultant biomaterials [ 11 ] or textiles [ 12 ]. SER is a highly hydrophilic glue-like glycoprotein with high serine and threonine contents [ 13 ].…”