Bioinformatics Database Resources

Khandelwal, Icxa; Sharma, Aditi; Agrawal, P. K.; Shrivastava, Rahul

doi:10.4018/978-1-5225-8903-7.ch004

Cited by 1 publication

(2 citation statements)

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“…BLAST is a tool used to compare sequences of various types of organisms. The score of each alignment is given an expectation value ( E value), which is a measure of statistical significance [ 54 ]. Pfam analysis …”

Section: Main Textmentioning

confidence: 99%

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Metagenomic applications in exploration and development of novel enzymes from nature: a review

Prayogo

Budiharjo

Kusumaningrum

et al. 2020

Journal of Genetic Engineering and Biotechnology

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Background: Microbial community has an essential role in various fields, especially the industrial sector. Microbes produce metabolites in the form of enzymes, which are one of the essential compounds for industrial processes. Unfortunately, there are still numerous microbes that cannot be identified and cultivated because of the limitations of the culture-based method. The metagenomic approach is a solution for researchers to overcome these problems. The main body of the abstract: Metagenomics is a strategy used to analyze the genomes of microbial communities in the environment directly. Metagenomics application used to explore novel enzymes is essential because it allows researchers to obtain data on microbial diversity, reaching of 99% and various types of genes encoding an enzyme that has not yet been identified. Basic methods in metagenomics have been developed and are commonly used in various studies. A basic understanding of metagenomics for researchers is needed, especially young researchers to support the success of the research. Short conclusion: Therefore, this review was done in order to provide a deep understanding of metagenomics. It also discussed the application and basic methods of metagenomics in the exploration of novel enzymes, especially in the latest research. Several types of enzymes, such as cellulases, proteases, and lipases, which have been explored using metagenomics, were reviewed in this article.

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Section: Main Textmentioning

confidence: 99%

“…Pfam’s analysis refers to the double alignment produced using the hidden Markov model. The purpose of Pfam’s analysis is to look at the relationship between protein sequences at the family level [ 54 ]. Analysis of protein structure prediction …”

Section: Main Textmentioning

confidence: 99%