Bioinformatics Analysis of Prognostic miRNA Signature and Potential Critical Genes in Colon Cancer

Chen, Weigang; Gao, Chang; Liu, Yong; Wen, Ying; Hong, Xiaoling; Huang, Zunnan

doi:10.3389/fgene.2020.00478

Cited by 34 publications

(29 citation statements)

References 91 publications

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“…Highlighted in this work, ribosome targeting is a novel function of miR-16-5p that may explain the tumor-suppressor properties of this miRNA. This effect was found through searching in two independent databases, which have, previously, provided critical resources for the identification of actionable miRNAs in various types of cancer and in diet and in physical activity [ 37 , 38 , 39 , 40 , 41 ]. Moreover, studies over the past years have shown that ribosomes are heavily modified in malignancies in order to meet the increment metabolic needs of the cancer cell.…”

Section: Discussionmentioning

confidence: 99%

miR-16-5p Promotes Erythroid Maturation of Erythroleukemia Cells by Regulating Ribosome Biogenesis

2021

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miRNAs constitute a class of non-coding RNA that act as powerful epigenetic regulators in animal and plant cells. In order to identify putative tumor-suppressor miRNAs we profiled the expression of various miRNAs during differentiation of erythroleukemia cells. RNA was purified before and after differentiation induction and subjected to quantitative RT-PCR. The majority of the miRNAs tested were found upregulated in differentiated cells with miR-16-5p showing the most significant increase. Functional studies using gain- and loss-of-function constructs proposed that miR-16-5p has a role in promoting the erythroid differentiation program of murine erythroleukemia (MEL) cells. In order to identify the underlying mechanism of action, we utilized bioinformatic in-silico platforms that incorporate predictions for the genes targeted by miR-16-5p. Interestingly, ribosome constituents, as well as ribosome biogenesis factors, were overrepresented among the miR-16-5p predicted gene targets. Accordingly, biochemical experiments showed that, indeed, miR-16-5p could modulate the levels of independent ribosomal proteins, and the overall ribosomal levels in cultured cells. In conclusion, miR-16-5p is identified as a differentiation-promoting agent in erythroleukemia cells, demonstrating antiproliferative activity, likely as a result of its ability to target the ribosomal machinery and restore any imbalanced activity imposed by the malignancy and the blockade of differentiation.

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Section: Discussionmentioning

confidence: 99%

miR-16-5p Promotes Erythroid Maturation of Erythroleukemia Cells by Regulating Ribosome Biogenesis

2021

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“…Several studies have reported that the expression of miR-3677 is negatively associated with the survival of patients with HCC and that miR-3677 is one of the tumor-specific miRNAs in HCC (12)(13)(14)(15). In addition, the preliminary prognostic role of miR-3677 has been determined in colon cancer (16,17). As a novel miRNA, miR-3677-5p has not been previously reported in neoplasms, to the best of our knowledge.…”

Section: Introductionmentioning

confidence: 87%

miR‑3677‑5p promotes the proliferation, migration and invasion of hepatocellular carcinoma cells and is associated with prognosis

et al. 2021

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MicroRNA (miRNA/miR)-3677 has been indicated to be negatively associated with the survival of patients with hepatocellular carcinoma (HCC) based on The Cancer Genome Atlas database. However, as a novel miRNA, the role of miR-3677-5p in HCC has remained to be elucidated. In the present study, the expression of miR-3677-5p was assessed in HCC tissues and cell lines using reverse transcription-quantitative PCR. Survival analysis was performed using Kaplan-Meier curves. Furthermore, the prognostic significance of miR-3677-5p was evaluated using Cox regression analysis. The effects of miR-3677-5p on cell proliferation, as well as migration and invasion capacities, were analyzed using Cell Counting Kit-8, crystal violet and Transwell assays. The results demonstrated that the level of miR-3677-5p expression was upregulated in human HCC tissues and cell lines and that miR-3677-5p expression was closely associated with tumor size, TNM stage and vascular invasion. Furthermore, high miR-3677-5p expression was significantly associated with unfavorable clinical prognosis for patients with HCC. Overexpression of miR-3677-5p was indicated to significantly promote the proliferation, migration and invasion of HCC cells, whereas knockdown of miR-3677-5p was observed to have an inhibitory effect. In conclusion, the present study demonstrated that miR-3677-5p acts as an oncogene that has a critical role in the regulation of HCC proliferation and progression. Hence, miR-3677-5p may serve as a valuable prognostic biomarker and may be developed as a promising therapeutic target for HCC. miR-3677-5p promotes the proliferation, migration and invasion of hepatocellular carcinoma cells and is associated with prognosis

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“…These show that elevated RHAMM expression is characteristic of most cancers and prognostic of a poor outcome in many. For example, elevated RHAMM expression is an indicator of poor prognosis in breast cancer [ 119 , 120 , 121 ], multiple myeloma [ 122 ], oral squamous cell carcinoma [ 123 ], ovarian cancer [ 124 ], lung cancer [ 125 ], prostate cancer [ 126 ], colon cancer [ 127 ], and gastric cancer [ 128 ]. However, histology analyses of these human tumors reveal that RHAMM expression is heterogeneous, and high RHAMM expression is often limited to a small subset of cancer cells [ 119 ].…”

Section: Rhamm Multi-functions In Cancermentioning

confidence: 99%

RHAMM Is a Multifunctional Protein That Regulates Cancer Progression

Messam

Tölg

McCarthy³

et al. 2021

IJMS

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The functional complexity of higher organisms is not easily accounted for by the size of their genomes. Rather, complexity appears to be generated by transcriptional, translational, and post-translational mechanisms and tissue organization that produces a context-dependent response of cells to specific stimuli. One property of gene products that likely increases the ability of cells to respond to stimuli with complexity is the multifunctionality of expressed proteins. Receptor for hyaluronan-mediated motility (RHAMM) is an example of a multifunctional protein that controls differential responses of cells in response-to-injury contexts. Here, we trace its evolution into a sensor-transducer of tissue injury signals in higher organisms through the detection of hyaluronan (HA) that accumulates in injured microenvironments. Our goal is to highlight the domain and isoform structures that generate RHAMM’s function complexity and model approaches for targeting its key functions to control cancer progression.

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Bioinformatics Analysis of Prognostic miRNA Signature and Potential Critical Genes in Colon Cancer

Cited by 34 publications

References 91 publications

miR-16-5p Promotes Erythroid Maturation of Erythroleukemia Cells by Regulating Ribosome Biogenesis

miR-16-5p Promotes Erythroid Maturation of Erythroleukemia Cells by Regulating Ribosome Biogenesis

miR‑3677‑5p promotes the proliferation, migration and invasion of hepatocellular carcinoma cells and is associated with prognosis

RHAMM Is a Multifunctional Protein That Regulates Cancer Progression

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