miR-16-5p Promotes Erythroid Maturation of Erythroleukemia Cells by Regulating Ribosome Biogenesis

Papagiannopoulos, C; Theodoroula, Nikoleta F.; Vizirianakis, Ioannis S.

doi:10.3390/ph14020137

Cited by 7 publications

(4 citation statements)

References 56 publications

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“…Merkerova et al (2008) found that miR-16 was highly expressed in most human hematopoietic cells [ 6 ]. There are similar reports regarding miR-16-5p [ 66 ]. Although miR-16–2 is associated with polychromatic erythroblast differentiation, details of their role have not been established yet [ 4 ].…”

Section: Mirna Regulation In Erythropoiesis (Table 2 ...supporting

confidence: 75%

Insight into microRNAs’ involvement in hematopoiesis: current standing point of findings

Nassiri,

Ahmadi Afshar,

Almasi

2023

Stem Cell Res Ther

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Hematopoiesis is a complex process in which hematopoietic stem cells are differentiated into all mature blood cells (red blood cells, white blood cells, and platelets). Different microRNAs (miRNAs) involve in several steps of this process. Indeed, miRNAs are small single-stranded non-coding RNA molecules, which control gene expression by translational inhibition and mRNA destabilization. Previous studies have revealed that increased or decreased expression of some of these miRNAs by targeting several proto-oncogenes could inhibit or stimulate the myeloid and erythroid lineage commitment, proliferation, and differentiation. During the last decades, the development of molecular and bioinformatics techniques has led to a comprehensive understanding of the role of various miRNAs in hematopoiesis. The critical roles of miRNAs in cell processes such as the cell cycle, apoptosis, and differentiation have been confirmed as well. However, the main contribution of some miRNAs is still unclear. Therefore, it seems undeniable that future studies are required to focus on miRNA activities during various hematopoietic stages and hematological malignancy.

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Section: Mirna Regulation In Erythropoiesis (Table 2 ...supporting

confidence: 75%

Insight into microRNAs’ involvement in hematopoiesis: current standing point of findings

Nassiri,

Ahmadi Afshar,

Almasi

2023

Stem Cell Res Ther

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“…* When the Ct values were undermined due to very low expression, a value of 40 was denoted for calculation. References [ 9 , 20 , 22 , 32 , 41 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 ] are referred to in the Supplementary Material file.…”

mentioning

confidence: 99%

Comprehensive Analysis of microRNAs in Human Adult Erythropoiesis

Nath

Rayabaram

Ijee

et al. 2021

Cells

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MicroRNAs (miRNAs) are small non-coding RNAs, which play an important role in various cellular and developmental processes. The study of miRNAs in erythropoiesis is crucial to uncover the cellular pathways that are modulated during the different stages of erythroid differentiation. Using erythroid cells derived from human CD34+ hematopoietic stem and progenitor cells (HSPCs)and small RNA sequencing, our study unravels the various miRNAs involved in critical cellular pathways in erythroid maturation. We analyzed the occupancy of erythroid transcription factors and chromatin accessibility in the promoter and enhancer regions of the differentially expressed miRNAs to integrate miRNAs in the transcriptional circuitry of erythropoiesis. Analysis of the targets of the differentially expressed miRNAs revealed novel pathways in erythroid differentiation. Finally, we described the application of Clustered regularly interspaced short palindromic repeats-Cas9 (CRISPR-Cas9) based editing of miRNAs to study their function in human erythropoiesis.

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“…By knockdown of miR‐16‐5p, we proved that miR‐16‐5p is essential for promoting aerobic glycolysis during sepsis. miR‐16‐5p directly binds to multiple target genes and modulates their expression (Cai et al, 2018; Papagiannopoulos et al, 2021; Qu et al, 2018; Ruan & Qian, 2019). However, its target in the macrophage, especially during infection, remains elusive.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‐16‐5p exacerbates sepsis by upregulating aerobic glycolysis via SIRT3‐SDHA axis

Huang

Yang

et al. 2022

Cell Biology International

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Sepsis is a life-threatening condition, and treatment for sepsis in clinic is often not available, partially due to insufficient understanding of the pathogenesis of sepsis.Extensive study to elucidate the pathogenesis is required to improve the clinical management and outcome of sepsis. In this study, we investigated the pathogenesis of sepsis using peripheral blood mononuclear cells (PBMCs) from septic patients and studied the underlying mechanism of miR-16-5p on aerobic glycolysis in lipopolysaccharide (LPS)-treated THP1 and Raw264.7 cells. The levels of RNA and protein were determined by real-time quantitative PCR and immunoblotting assay, respectively. The production of high mobility group box 1 (HMGB1) was measured by enzyme-linked immunosorbent assay. The levels of succinate and lactate were determined using colorimetric kits. The extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) were measured by extracellular flux analyzer. The results showed that the expression of miR-16-5p was elevated, while sirtuin 3 (SIRT3) was decreased in PBMCs from septic patients and LPS-treated cells, along with accumulation of acetylated succinate dehydrogenase complex, subunit A.Concomitantly, an increase in HMGB1, succinate, lactate, as well as ECAR and a decrease in OCR were observed. Knockdown of miR-16-5p upregulated SIRT3 expression, facilitated SDHA deacetylation, and attenuated sepsis-related aerobic glycolysis. Further study identified that SIRT3 is targeted by miR-16-5p, and overexpression of SIRT3 rescued LPS-induced responses via deacetylation of SDHA.Our findings revealed a novel miR-16-5p-regulated SIRT3-SDHA axis in sepsis and provided novel insights for sepsis treatment.

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miR-16-5p Promotes Erythroid Maturation of Erythroleukemia Cells by Regulating Ribosome Biogenesis

Cited by 7 publications

References 56 publications

Insight into microRNAs’ involvement in hematopoiesis: current standing point of findings

Insight into microRNAs’ involvement in hematopoiesis: current standing point of findings

Comprehensive Analysis of microRNAs in Human Adult Erythropoiesis

MicroRNA‐16‐5p exacerbates sepsis by upregulating aerobic glycolysis via SIRT3‐SDHA axis

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