Bioinformatics analysis of microRNA profiles and identification of microRNA-mRNA network and biological markers in intracranial aneurysm

Abstract

Intracranial aneurysm (IA) is a kind of cerebrovascular disorder, which may result in the subarachnoid hemorrhage with high lethality and disability. The purpose of this study was to reveal the pathogenesis and identify novel biomarkers in IA. We processed the raw microRNA (miRNA) expression profile data of IA obtained from Gene Expression Omnibus. Then weighted correlation network analysis was performed to identify the hub miRNAs in IA. Target genes of hub miRNAs were predicted using multiR package. In addition, a protein-protein network as well as miRNA-mRNA network was constructed and functional and pathway enrichment analyses were done. Finally, the prediction value of hub miRNAs in IA was tested in validation set. Two modules that had relation with IA were identified and 10 hub miRNAs in each module with higher gene-module association were selected. The protein-protein network and miRNA-mRNA network contained 243 nodes and 1496 edges. Functional and pathway enrichment analyses showed that they were mainly enriched in cell cycle, cell proliferation, and PI3K/Akt signaling pathways. Besides, hsa-miR-191-3p, hsa-miR-423-5p, hsa-miR-424-5p, hsa-miR-425-3p were proven to be valuable in prediction IA occurrence. In a word, this study reveals hub miRNAs, target genes and pathways potentially participating in formation and development of IA and screens out some candidate biomarkers. Our findings provide some new perspectives for research and treatment of IA.

Functional Significance of Cardiac Rehabilitation-Regulated Expression of Circulating MicroRNA-423-5p in Hypertensive Patients with Heart Failure with a Moderately Reduced Ejection Fraction

“…23 , 24 Analysis of the expression of VEGF, Bcl-2, and MT1-MMP in this research revealed that miR-423-5p was a good predictor of HFmrEF, which could accurately indicate an improved cardiac function in those patients following cardiac rehabilitation treatment. Combined with previous studies, our data suggest that miR-423-5p may induce cardiomyocyte apoptosis by regulating the PI3k/AKT pathway 25 and inhibiting O-type N -acetylglucosaminyltransferase (OGT). 26 Meanwhile, cardiac rehabilitation may improve cardiac function, promote physiological structural changes in the heart, and inhibit cardiac physiological remodeling 27 by regulating endothelial progenitor cells via miR-423-5p.…”

The Role of Epigenetics in Brain Aneurysm and Subarachnoid Hemorrhage: A Comprehensive Review

“…A primary limitation in these studies remains the small sample sizes within the GEO datasets explored. However, it is noteworthy that some studies have validated their findings in distinct GEO datasets [ 101 , 102 , 103 ].…”

Upregulated anti-angiogenic miR-424-5p in type 1 diabetes (model of subclinical cardiovascular disease) correlates with endothelial progenitor cells, CXCR1/2 and other parameters of vascular health

“…Despite the small study size, this independent analysis can be used to confirm that miR-424-5p is a biomarker for the beginning of CVD. Others also reported miR-424-5p to be a biological marker of intracranial aneurism [ 50 ] or predictor of risk of fatal myocardial infarction whilst combined with Framingham score [ 51 ].…”