Bioinformatics analysis of aberrantly expressed exosomal lncRNAs in oral squamous cell carcinoma (CAL‑27 vs. oral epithelial) cells

Abstract

Oral squamous cell carcinoma (OSCC) is the most prevalent form of malignant tumour in the oral cavity and its early detection is critical for improving the prognosis of affected patients. The present study aimed to isolate and confirm exosomes derived from the supernatant of the OSCC cell line CAL-27 and human oral epithelial cells (HOECs), analyze long non-coding RNA (lncRNA) expression profiles and determine the diagnostic value based on bioinformatics analyses. The results indicated that the particles isolated from the supernatant of CAL-27 and HOECs were either round or oval, had a size range of 30-150 nm and were enriched with ALG-2 interacting protein X (ALIX) and tumour susceptibility 101 proteins (TSG101). These characteristics confirmed that these particles were exosomes. Three lncRNAs (NR-026892.1, NR-126435.1 and NR-036586.1) were selected as potential diagnostic biomarkers for OSCC. The expression levels of the selected lncRNAs were significantly different in CAL-27-exo vs. HOEC-exo, as well as in whole cells (CAL-27 vs. HOECs) (P<0.001). The expression levels of the three lncRNAs confirmed by quantitative PCR were consistent with the sequencing data. In conclusion, various lncRNAs were aberrantly expressed between cancerous and non-cancerous exosomes, suggesting that they may serve as biomarkers for cancer.

Establishment of a Livin-gene silencing system and its effect on the sensitivity of Cal 27 with regard to 5-fluorouracil

“…Human oral epithelial cells (HOECs) and periodontal ligament cells (PDLCs) were used in accordance with the ethical standards established in the Declaration of Helsinki. HOECs and PDLCs were stored, thawed and cultured as previously described [19][20][21]. HOECs were cultured in EpiGRO™ Human Epidermal Keratinocyte Complete Media Kit (Merck Millipore, Germany), and the medium for PDLCs and Cal 27 was α-MEM (Gibco, US) and DMEM (Gibco, US), respectively, and 10% (v/v) was supplemented with fetal bovine serum (FBS, Gibco, USA).…”

Understanding exosomes: Part 3—therapeutic + diagnostic potential in dentistry

“…The possible relationship between this microRNA and the characteristics and actions of oral tumors that express it may be better understood in light of these studies. Future studies will look at these possible routes and processes to see whether miR-365 may be used as a biomarker for oral cancer in salivary or liquid biopsies Jin et al (2020) 134 We selected three long noncoding RNAs (lncRNAs) (NR 026892. The current study's findings showed three micro-RNAs that may have a significant impact on the TP53 gene's activities and cause dangerous consequences for patients with HNSCC.…”