Profiling of extracellular vesicles in oral cancer, from transcriptomics to proteomics

Leung, Leanne; Riaz, Muhammad Kashif; Qu, Xinyu; Chan, Jason Ying Kuen; Meehan, Katie

doi:10.1016/j.semcancer.2021.01.002

Cited by 18 publications

(12 citation statements)

References 76 publications

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“…Compared with the normal tissues, the miRNA profiles of the tumor tissues generally exhibited significant change [ 13 ]. Accumulating researches have indicated that some miRNAs serve critical roles in the deterioration of the tumors [ 14 ]. In the research, it was also found that the miRNA profiles were dramatically difference in the tumor tissues and adjacent healthy tissues, and 7 downregulated genes and 28 upregulated were identified as the DEG miRNAs which may serve as critical roles in oral cancer.…”

Section: Discussionmentioning

confidence: 99%

Identification of Potential miRNA-mRNA Regulatory Network in the Development of Oral Cancer

Yang

Xin

2022

Disease Markers

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Background. Oral cancer is a difficult question in modern medical system, and there are few effective strategies to completely heal these diseases. This research investigated the miRNA-mRNA network in oral cancer development via bioinformatics excavation. Methods. GSE28100 and GSE23558 in the GEO database were selected for bioinformatics analysis. The datasets were analyzed with GEO2R to obtain the related matrix files. The hot plot and heatmap of the matrix files were drawn with R language. The MiRDIP database was applied to predict and screen the targets of miRNAs. The DEGs in the matrix files were analyzed with the DAVID database and visualized with R language for enrichment analysis. The PPI-network of the DEGs was established with the STRING database and Cytoscape. Besides, the miRNA-mRNA was visualized by Cytoscape. Results. 35 genes were identified as the DEGs in GES28100. 1651 genes were identified as the DEGs in GSE23558. 143 common genes in the targets of miRNAs in GSE28100 and the DEGs in GSE the targets of DEGs in GES28100 and common genes were enriched in the PI3K/AKT pathway, MAPK pathway, etc. The DEGs in GSE28100 and GSE23558 were involved in the regulations of transcription from RNA polymerase II promoter and DNA transcription. The DEGs in GSE28100 and GSE23558 were established with the miRNA-mRNA network. Conclusion. This research identified miR-15b-5p, miR-199a-3p, miR-21-5p, miR-424-5p, and miR-454-3p as the biomarker of oral cancer and established the miRNA-mRNA network in oral progression.

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Section: Discussionmentioning

confidence: 99%

Identification of Potential miRNA-mRNA Regulatory Network in the Development of Oral Cancer

Yang

Xin

2022

Disease Markers

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“…In terms of diagnostics, this can be addressed by improvements of detection sensitivity and specificity, while for potential therapeutics, in vitro amplification of Ti‐EVs may represent a potent and practical solution. Second, an invasive sampling process cannot be avoided (Leung et al., 2021 ). Moreover, sampling for Ti‐EVs may be unavailable in some instances, including deep malignant tumours and tumours that are not suitable for biopsy, such as arterial aneurysms.…”

Section: Problems and Solutionsmentioning

confidence: 99%

“…Compared with cell culture‐derived EVs, growing attention has focused on EVs obtained from various biological fluids, such as blood, breast milk, cerebrospinal fluid, urine, and saliva (Belov et al., 2016 , Guo et al., 2020 , Leung et al., 2021 , Vasconcelos et al., 2019 ). Body fluid‐derived EVs offer a minimally invasive way to reflect the dynamic progression of diseases in real‐time (Leung et al., 2021 ). However, admixtures from multiple sources are contained in body fluids, including serum proteins or a mixture of EVs from the whole body (Jingushi et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Tissue‐derived extracellular vesicles in cancers and non‐cancer diseases: Present and future

Man

Gao

et al. 2021

J of Extracellular Vesicle

110

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Extracellular vesicles (EVs) are lipid‐bilayer membrane structures secreted by most cell types. EVs act as messengers via the horizontal transfer of lipids, proteins, and nucleic acids, and influence various pathophysiological processes in both parent and recipient cells. Compared to EVs obtained from body fluids or cell culture supernatants, EVs isolated directly from tissues possess a number of advantages, including tissue specificity, accurate reflection of tissue microenvironment, etc., thus, attention should be paid to tissue‐derived EVs (Ti‐EVs). Ti‐EVs are present in the interstitium of tissues and play pivotal roles in intercellular communication. Moreover, Ti‐EVs provide an excellent snapshot of interactions among various cell types with a common histological background. Thus, Ti‐EVs may be used to gain insights into the development and progression of diseases. To date, extensive investigations have focused on the role of body fluid‐derived EVs or cell culture‐derived EVs; however, the number of studies on Ti‐EVs remains insufficient. Herein, we summarize the latest advances in Ti‐EVs for cancers and non‐cancer diseases. We propose the future application of Ti‐EVs in basic research and clinical practice. Workflows for Ti‐EV isolation and characterization between cancers and non‐cancer diseases are reviewed and compared. Moreover, we discuss current issues associated with Ti‐EVs and provide potential directions.

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“…Recent studies have shown that extracellular vesicles (EVs) secreted by tumor cells are key messengers for inducing tumor vascular permeability, inflammation, and recruitment of bone marrow progenitor cells in remote organs for cellular communication [ 6 , 7 , 8 ]. For example, EVs containing EGFR regulate the liver microenvironment and promote liver metastasis [ 9 ], EV miR-25-3p is involved in the formation of a pre-metastatic niche before metastasis [ 10 ], and EV integrin expression facilitates tumor homing to specific distant metastatic sites [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Circulating Extracellular Vesicles Identifies Potential Biomarkers for Lymph Node Metastasis in Oral Tongue Squamous Cell Carcinoma

Leung

Ngai

et al. 2021

Cells

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Lymph node metastasis is the most reliable indicator of a poor prognosis for patients with oral tongue cancers. Currently, there are no biomarkers to predict whether a cancer will spread in the future if it has not already spread at the time of diagnosis. The aim of this study was to quantitatively profile the proteomes of extracellular vesicles (EVs) isolated from blood samples taken from patients with oral tongue squamous cell carcinoma with and without lymph node involvement and non-cancer controls. EVs were enriched using size exclusion chromatography (SEC) from pooled plasma samples of patients with non-nodal and nodal oral tongue squamous cell carcinoma (OTSCC) and non-cancer controls. Protein cargo was quantitatively profiled using isobaric labelling (iTRAQ) and two-dimensional high-performance liquid chromatography followed by tandem mass spectrometry. We identified 208 EV associated proteins and, after filtering, generated a short list of 136 proteins. Over 85% of the EV-associated proteins were associated with the GO cellular compartment term “extracellular exosome”. Comparisons between non-cancer controls and oral tongue squamous cell carcinoma with and without lymph node involvement revealed 43 unique candidate EV-associated proteins with deregulated expression patterns. The shortlisted EV associated proteins described here may be useful discriminatory biomarkers for differentiating OTSCC with and without nodal disease or non-cancer controls.

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Profiling of extracellular vesicles in oral cancer, from transcriptomics to proteomics

Cited by 18 publications

References 76 publications

Identification of Potential miRNA-mRNA Regulatory Network in the Development of Oral Cancer

Identification of Potential miRNA-mRNA Regulatory Network in the Development of Oral Cancer

Tissue‐derived extracellular vesicles in cancers and non‐cancer diseases: Present and future

Proteomic Analysis of Circulating Extracellular Vesicles Identifies Potential Biomarkers for Lymph Node Metastasis in Oral Tongue Squamous Cell Carcinoma

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