Bioimaging tools move plant physiology studies forward

Hsiao, An‐Shan; Huang, Ji-Ying

doi:10.3389/fpls.2022.976627

Cited by 4 publications

(3 citation statements)

References 88 publications

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“…With their multiple roles explored, the next step is to apply modern technologies in cell biology and biophysics for understanding the underlying mechanism [228][229][230][231][232]. Bioimaging tools are useful for studying plant physiological processes in vivo [233]. A recent report suggested that fixation causes artifacts of LLPS in the animal system [234], so live-cell imaging could be a preferable approach to dissect the phenomenon of LLPS.…”

Section: Discussionmentioning

confidence: 99%

Protein Disorder in Plant Stress Adaptation: From Late Embryogenesis Abundant to Other Intrinsically Disordered Proteins

Hsiao

2024

IJMS

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Global climate change has caused severe abiotic and biotic stresses, affecting plant growth and food security. The mechanical understanding of plant stress responses is critical for achieving sustainable agriculture. Intrinsically disordered proteins (IDPs) are a group of proteins without unique three-dimensional structures. The environmental sensitivity and structural flexibility of IDPs contribute to the growth and developmental plasticity for sessile plants to deal with environmental challenges. This article discusses the roles of various disordered proteins in plant stress tolerance and resistance, describes the current mechanistic insights into unstructured proteins such as the disorder-to-order transition for adopting secondary structures to interact with specific partners (i.e., cellular membranes, membrane proteins, metal ions, and DNA), and elucidates the roles of liquid–liquid phase separation driven by protein disorder in stress responses. By comparing IDP studies in animal systems, this article provides conceptual principles of plant protein disorder in stress adaptation, reveals the current research gaps, and advises on the future research direction. The highlighting of relevant unanswered questions in plant protein disorder research aims to encourage more studies on these emerging topics to understand the mechanisms of action behind their stress resistance phenotypes.

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Section: Discussionmentioning

confidence: 99%

Protein Disorder in Plant Stress Adaptation: From Late Embryogenesis Abundant to Other Intrinsically Disordered Proteins

Hsiao

2024

IJMS

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“…Based on multiband spectral data, hyperspectral microscopy can realize the distribution and quantitative analysis of proteins and small molecules (Fakhrullin et al ., 2021 ). Infrared‐based microscopy allows for the quantitative visualization of sucrose in individual vascular bundles and within a complex organ such as the stem, leaf, or seed (Hsiao and Huang, 2022 ). Raman microscopy has been employed to visualize cellulose and pectin in plant cell walls (Cui et al ., 2023 ).…”

Section: Imaging Modalities For Plant Microphenotypesmentioning

confidence: 99%

Plant microphenotype: from innovative imaging to computational analysis

Zhang,

Gu,

et al. 2024

Plant Biotechnology Journal

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SummaryThe microphenotype plays a key role in bridging the gap between the genotype and the complex macro phenotype. In this article, we review the advances in data acquisition and the intelligent analysis of plant microphenotyping and present applications of microphenotyping in plant science over the past two decades. We then point out several challenges in this field and suggest that cross‐scale image acquisition strategies, powerful artificial intelligence algorithms, advanced genetic analysis, and computational phenotyping need to be established and performed to better understand interactions among genotype, environment, and management. Microphenotyping has entered the era of Microphenotyping 3.0 and will largely advance functional genomics and plant science.

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“…Understanding the response patterns of these secondary metabolites to potential global climate changes is crucial due to their significance in plant growth, resistance, human health, and conservation efforts. Recently developed powerful omics techniques [33][34][35] and bioimaging [36] and biosensor tools [37,38] have been widely applied for understanding plant physiology, analysing the plant metabolome, and discovering novel metabolomic pathways in response to the changing environment [39]. However, there is no comprehensive review that examined recent metabolomic and phytochemical studies on climateaffected plants of Australian TMCF.…”

Section: Introductionmentioning

confidence: 99%

Climate-Affected Australian Tropical Montane Cloud Forest Plants: Metabolomic Profiles, Isolated Phytochemicals, and Bioactivities

Gempo,

Yeshi,

Crayn

et al. 2024

Plants

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The Australian Wet Tropics World Heritage Area (WTWHA) in northeast Queensland is home to approximately 18 percent of the nation’s total vascular plant species. Over the past century, human activity and industrial development have caused global climate changes, posing a severe and irreversible danger to the entire land-based ecosystem, and the WTWHA is no exception. The current average annual temperature of WTWHA in northeast Queensland is 24 °C. However, in the coming years (by 2030), the average annual temperature increase is estimated to be between 0.5 and 1.4 °C compared to the climate observed between 1986 and 2005. Looking further ahead to 2070, the anticipated temperature rise is projected to be between 1.0 and 3.2 °C, with the exact range depending on future emissions. We identified 84 plant species, endemic to tropical montane cloud forests (TMCF) within the WTWHA, which are already experiencing climate change threats. Some of these plants are used in herbal medicines. This study comprehensively reviewed the metabolomics studies conducted on these 84 plant species until now toward understanding their physiological and metabolomics responses to global climate change. This review also discusses the following: (i) recent developments in plant metabolomics studies that can be applied to study and better understand the interactions of wet tropics plants with climatic stress, (ii) medicinal plants and isolated phytochemicals with structural diversity, and (iii) reported biological activities of crude extracts and isolated compounds.

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Bioimaging tools move plant physiology studies forward

Cited by 4 publications

References 88 publications

Protein Disorder in Plant Stress Adaptation: From Late Embryogenesis Abundant to Other Intrinsically Disordered Proteins

Protein Disorder in Plant Stress Adaptation: From Late Embryogenesis Abundant to Other Intrinsically Disordered Proteins

Plant microphenotype: from innovative imaging to computational analysis

Climate-Affected Australian Tropical Montane Cloud Forest Plants: Metabolomic Profiles, Isolated Phytochemicals, and Bioactivities

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