Biohydrogen production performance in a draft tube bioreactor with immobilized cell

Chu, Chen-Yeon; Wu, Shu-Yii; Shen, Yuan-Chang

doi:10.1016/j.ijhydene.2012.03.031

Cited by 19 publications

(6 citation statements)

References 20 publications

(11 reference statements)

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“…This behavior has been observed in ceramic materials such as bricks and glasses [ 89 ]. This behavior is somewhat counterintuitive as we expected that highly crosslinked hydrogels exhibited the highest resistance to rupture [ 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 ,…”

Section: Resultsmentioning

confidence: 99%

“…Some of the preferred species include Saccharomyces , Kluyveromyces and Lactobacillus [ 16 , 33 , 34 ]. Bubble columns and both concentric-tube and external-loop airlift bioreactors have been extensively used [ 35 , 36 , 37 , 38 ]. With this approach, it has been possible to produce numerous metabolites of commercial interest such as bioethanol, cellulose, biohydrogen, oxalic acid, gluconic acid, citric acid, malic acid and lactic acid [ 35 , 36 , 37 , 39 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

“…Bubble columns and both concentric-tube and external-loop airlift bioreactors have been extensively used [ 35 , 36 , 37 , 38 ]. With this approach, it has been possible to produce numerous metabolites of commercial interest such as bioethanol, cellulose, biohydrogen, oxalic acid, gluconic acid, citric acid, malic acid and lactic acid [ 35 , 36 , 37 , 39 , 40 ]. Additionally, it has been possible to improve the quality of wastewaters by reducing the contents of contaminants, such as heavy and cationic metals, phenol and dyes [ 41 , 42 ].…”

Section: Introductionmentioning

confidence: 99%

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Formulation and Characterization of Gelatin-Based Hydrogels for the Encapsulation of Kluyveromyces lactis—Applications in Packed-Bed Reactors and Probiotics Delivery in Humans

Patarroyo¹,

Florez-Rojas²,

Pradilla³

et al. 2020

Polymers

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One of the main issues when orally administering microorganism-based probiotics is the significant loss of bioactivity as they pass through the gastrointestinal (GI) tract. To overcome these issues, here, we propose to encapsulate the probiotic yeast Kluyveromyces lactis on chemically crosslinked gelatin hydrogels as a means to protect the bioactive agents in different environments. Hydrogels were prepared by the chemical crosslinking of gelatin, which is commercially available and inexpensive. This is crucial to ensure scalability and cost-effectiveness. To explore changes in key physicochemical parameters and their impact on cell viability, we varied the concentration of the crosslinking agent (glutaraldehyde) and the gelatin. The synthesized hydrogels were characterized in terms of morphological, physical-chemical, mechanical, thermal and rheological properties. This comprehensive characterization allowed us to identify critical parameters to facilitate encapsulation and enhance cell survival. Mainly due to pore size in the range of 5–10 μm, sufficient rigidity (breaking forces of about 1 N), low brittleness and structural stability under swelling and relatively high shear conditions, we selected hydrogels with a high concentration of gelatin (7.5% (w/v)) and concentrations of the crosslinking agent of 3.0% and 5.0% (w/w) for cell encapsulation. Yeasts were encapsulated with an efficiency of about 10% and subsequently tested in bioreactor operation and GI tract simulated media, thereby leading to cell viability levels that approached 95% and 50%, respectively. After testing, the hydrogels’ firmness was only reduced to half of the initial value and maintained resistance to shear even under extreme pH conditions. The mechanisms underlying the observed mechanical response will require further investigation. These encouraging results, added to the superior structural stability after the treatments, indicate that the proposed encapsulates are suitable to overcome most of the major issues of oral administration of probiotics and open the possibility to explore additional biotech applications further.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Formulation and Characterization of Gelatin-Based Hydrogels for the Encapsulation of Kluyveromyces lactis—Applications in Packed-Bed Reactors and Probiotics Delivery in Humans

Patarroyo¹,

Florez-Rojas²,

Pradilla³

et al. 2020

Polymers

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“…Considering the low biomass in suspended systems, biofilms or microbial aggregates were used in recent years for fermentative H 2 production since they accommodate higher active biomass and fermentation rate [12,[16][17][18][19] Since experiments in 250 mL serum bottles without mixing (not reported here) proved floc formation in pure culture of C. butyricum CWBI1009 after a few sequences of SBR, the experimentations reported in this paper aimed to study the ability of the pure strain to form microbial aggregates in different bioreactor systems. The investigations were first carried out…”

Section: /35mentioning

confidence: 99%

Section: /35mentioning

confidence: 99%

Improvement of fermentative biohydrogen production by Clostridium butyricum CWBI1009 in sequenced-batch, horizontal fixed bed and biodisc-like anaerobic reactors with biomass retention

Hiligsmann

Beckers

Masset

et al. 2014

International Journal of Hydrogen Energy

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A horizontal tubular fixed bed bioreactor (HFBR) and an anaerobic biodisc-like reactor (AnBDR) were designed to both fix Clostridium biomass and enable rapid transfer of the hydrogen produced to gas phase in order to decrease the strong effect of H 2 partial pressure and H 2 supersaturation on the performances of Clostridium strains. The highest H 2 production rate (703 mL H 2 /L.h) and yield (302 mL/g glucose consumed i.e. 2.4 mol/mol) with the pure culture were recorded in the AnBDR with 300 mL culture medium (total volume 2.3 L) at pH 5.2 and a glucose loading rate of 2.87 g/L.h. These results are about 2.3 and 1.3-fold higher than those achieved in the same bioreactor with 500 mL liquid medium and with the same glucose consumption rate. Therefore, our experimentations and a short review of the literature reported in this paper emphasize the relevance of performing bioreactors with high L/G transfer.Keywords: Clostridium; hydrogen production; biofilm; bioreactor; pure strain CWBI -ULg 2/35 IntroductionThe fermentative production of hydrogen has drawn increased attention in recent years. This biological process called "dark fermentation" (DF) offers new opportunities to produce "green" energy from various renewable resources and organic wastes [1][2][3]. While significant improvements have been made in development of such alternative H 2 production systems, more technical progress and cost reduction needs to occur for them to compete with current large scale technologies e.g. methane-reforming process. By contrast, for local and smaller scale DF and some other opportunities, biohydrogen production processes would be cost competitive since the feedstocks are available almost anywhere and crucial interest is paid for both energy independence and efficient utilization [4,5]. However, optimization is still needed for DF regarding the bioreactor design, rapid removal and purification of gases, use of cheaper feedstock, genetic and molecular engineering to redirect metabolic pathway [6][7][8][9][10].Moreover, DF is only likely to be viable as an industrial process if integrated with a process that maximizes energy recovery from the fermentation end-products. The traditional methaneproducing anaerobic digestion process is the most promising since about 10 to 30 % more energy could be generated in the two-stage integrated system comparing to a single stage methanogenic process [11]. Besides, very prospective processes to convert acetate from DF spent medium exist such as further biohydrogen production (towards the maximum theoretical yield of 12 mol/mol glucose) by photosynthetic non-sulfur bacteria or direct electricity production in microbial fuel cells [6,11].In the past decades, most studies on biohydrogen production processes dealt with suspended culture systems such as the conventional (dis-)continuous stirred tank reactors (CSTR) since they are relatively simple and easy to operate. These investigations, several times reviewed [5,10,12,13], enabled to optimize number of operating parameters such as the ...

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Biohydrogen Production via Lignocellulose and Organic Waste Fermentation

Chu

Huang

2015

Biofuels and Biorefineries

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Biohydrogen production performance in a draft tube bioreactor with immobilized cell

Cited by 19 publications

References 20 publications

Formulation and Characterization of Gelatin-Based Hydrogels for the Encapsulation of Kluyveromyces lactis—Applications in Packed-Bed Reactors and Probiotics Delivery in Humans

Formulation and Characterization of Gelatin-Based Hydrogels for the Encapsulation of Kluyveromyces lactis—Applications in Packed-Bed Reactors and Probiotics Delivery in Humans

Improvement of fermentative biohydrogen production by Clostridium butyricum CWBI1009 in sequenced-batch, horizontal fixed bed and biodisc-like anaerobic reactors with biomass retention

Biohydrogen Production via Lignocellulose and Organic Waste Fermentation

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