Bioguided Fractionation and Isolation of Free Radical Scavenging Components from in Vitro Propagated Chinese Medicinal Plants Dendrobium tosaense Makino and Dendrobium moniliforme SW

Abstract

This study was performed to investigate the free radical scavenging active components from in vitro propagated medicinal herbs of the genus Dendrobium, namely, Dendrobium tosaense Makino and Dendrobium moniliforme SW, using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical antioxidative assay. Seeds of the capsules derived after 12 weeks of hand-pollination germinated asymbiotically (50-74%) on half-strength Murashige and Skoog's (MS) basal medium with 3% sucrose and solidified with 0.9% Difco agar. Active growth in the germinated seedlings was achieved by reculturing on full-strength MS basal medium supplemented with 8% banana homogenate, 8% potato homogenate, 8% coconut water, 1.5% sucrose, and 0.9% Difco agar. Healthy plantlets transferred to plastic trays containing moss or moss and tree fern successfully acclimatized (84-100%) in the greenhouse. Extracts were prepared from plants grown in the greenhouse for a period of 6 months. Methanolic extracts of D. tosaense and D. moniliforme scavenged DPPH at 95.9 and 83.4%, respectively, at a concentration of 0.4 mg/mL. Therefore, methanolic solubles of D. tosaense and D. moniliforme were subjected to bioguided fractionation and separation by column chromatographic methods individually. After chromatographic separation of these crude extracts, the obtained fractions (Dm 1, Dm 2, Dm 3, Dt 1, Dt 2, and Dt 3) were tested for their activity. Among them, fractions Dm 2 and Dt 1 showed significant antioxidant activity by DPPH radical antioxidative assay. Active fractions were purified further by column chromatography and resulted in identification of the antioxidant components alkyl ferulates from D. moniliforme and quercetin from D. tosaense.

Pemanfaatan Anggrek Spesies Kalimantan Tengah Berbasis Kearifan Lokal yang Berpotensi sebagai Bahan Obat Herbal

“…Spesies Dendrobium diketahui menghasilkan bermacam-macam metabolit sekunder seperti phenanthren, bibenzyl, fluorenone dan sesquiterpene, serta alkaloid dan banyak diantaranya yang secara luas digunakan sebagai obat (Lo et al, 2004). Pada akhir tiga dekade, penelitian pada anggrek Dendrobium diisolasi menghasilkan komponen phenanthrenes antara lain dihydrophenanthrene, ephemeranthoquionone, shihunidine, shihunine, dendrophenol, moscatilin, moscatin, moscatilin, denfigenin, defuscin, amoenumin, moscatilin, crepaditin, rotundatin, cumulatin, dan gigantol (Majumder & Sen, 1987;Majumder & Chatterjee, 1989;Majumder & Pal, 1992;Honda & Yamaki, 2000;Krohn et al, 2001;Zhang et al, 2007, Li et al, 2008Hossain, 2011 Bagian organ anggrek A. graminifolia yang dimanfaatkan sebagai obat adalah organ rhizoma dan akar.…”

Whitening and Anti-wrinkle Effects of Korean Native Dendrobium moniliforme Methanol Extract

“…따라서 collagen 생성을 촉진하거나 collagen을 분해하는 collagenase나 다양한 기질 단백질 분해 효소인 matrix metalloproteinases (MMPs)의 발현이나 활성을 저해함 으로써 주름생성을 억제할 수 있다 (Fisher et al, 1999). 국내 자생 석곡은 난초과에 속한 다년생 초본으로 바위나 나무 위에 붙어사는 상록성 여러해살이풀이며, 주요 연구로는 약용석곡으로 알려진 금채석곡 (Dendrobium nobile Lindley) 의 항산화 효능에 관한 연구와 H 2 O 2 에 의해 손상된 신경세포 주에 대한 보호효과 그리고 항산화, 항염증, 미백, 주름개선 효 능에 대한 보고가 있다 (Kim et al, 2005;Lo et al, 2004;Min et al, 2010;Yoon et al, 2007). 그리고 Dendrobium 속 식물은 면역조절작용, 간보호효과, 항산화, 항암, 고혈압 등 의 작용이 있다고 알려져 있다 (Choi et al, 2002;Tzi et al, 2012).…”

Chemical Constituents of Dendrobium williamsonii

“…[4,5] Recent biological studies have shown that some Dendrobium species possess anti-platelet aggregation, anti-fibrotic, free radical scavenging, immunomodulatory and cytotoxic activities. [5][6][7][8] Dendrobium williamsonii Day &Rchb. f. is known in Thai as "Ueangngoen sad".…”