Pemanfaatan Anggrek Spesies Kalimantan Tengah Berbasis Kearifan Lokal yang Berpotensi  sebagai Bahan Obat Herbal

Wahyudiningsih, Tri Suwarni; Nion, Yanetri Asi; Pahawang,

doi:10.15575/biodjati.v2i2.1570

Cited by 8 publications

(5 citation statements)

References 12 publications

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“…Anggrek di Indonesia memiliki keanekaragaman sekitar lebih dari 5.000 jenis dari 40 genus (Febriandito & Soetopo, 2019). Jenis tumbuhan anggrek dari family Orchidaceae berpotensi sebagai bahan baku obat seperti penurun demam, tuberkulosis (TBC), rematik, sakit perut, dan pemulih stamina (Hossain, 2011;Wahyudiningsih et al, 2017).…”

Section: Pendahuluanunclassified

Evaluasi Lokus Potensial matK dan ITS2 Untuk DNA Barcoding Anggrek Bulbophyllum lobbii Lindl.

Su'udi,

Ulum,

Ardiyansah

et al. 2024

Al-Kauniyah J. Biol.

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AbstrakBulbophyllum lobbii Lindl. merupakan anggrek dari famili Orchidaceae yang berpotensi sebagai bahan baku obat herbal. Identikasi morfologi anggrek B. lobii memiliki keterbatasan karena kemiripan spesies dengan anggrek lain. Alternatif identifikasi secara molekuler menggunakan sekuen matK dan ITS2 sebagai barcode dalam DNA barcoding diharapkan menjadi salah satu lokus pembeda spesies anggrek B. lobbii secara akurat dan efisien. Penelitian ini bertujuan untuk mengidentifikasi sekuen matK dan ITS2 sebagai penanda molekuler yang efektif untuk anggrek B. lobbii. DNA genom B. lobbii diisolasi dengan metode Cethyl Trimethyl Ammonium Bromide (CTAB) dan amplifikasi DNA dengan PCR. Hasil penelitian menunjukkan sekuen matK dari B. lobbii memiliki tingkat homologi tinggi dengan dua spesies B. lobbii (KY966747.1 dan KY966691.1) dari China dengan nilai Per. Ident sebesar 99,20%, sedangkan sekuen ITS2 memiliki homologi tertinggi dengan nilai Per. Ident sebesar 99,76% pada spesies B. lobbii (MG253848.1) dari Polandia. Hasil analisis menunjukkan sekuen ITS2 dapat mengidentifikasi spesies dari tingkatan subspesies atau diatasnya (ordo atau genus) dan juga meningkatkan resolusi filogenetik yang baik pada hasil BLAST, sedangkan sekuen matK memberikan sedikit kontribusi dalam pengelompokkan hubungan kekerabatan antara spesies B. lobbii dengan spesies pembanding lainnya. Sekuen ITS2 dapat direkomendasikan sebagai penanda molekuler yang paling baik untuk identifikasi sampel anggrek Bulbophyllum, khususnya Bulbophyllum lobbii.AbstractBulbophyllum lobbii Lindl. is an orchid from the Orchidaceae family which has potential as a raw material for herbal medicine. Morphological identification of the B. lobii orchid has limitations due to the species' similarity to other orchids. The alternative molecular identification using matK and ITS2 sequences as barcodes in DNA barcoding is expected to be one of the loci for distinguishing the B. lobbii orchid species accurately and efficiently. This study aims to identify matK and ITS2 sequences as effective molecular markers for the orchid B. lobbii. Bulbophyllum lobbii genomic DNA was isolated using the Cethyl Trimethyl Ammonium Bromide (CTAB) method and DNA amplification by PCR. The results showed that the matK sequence from B. lobbii has a high level of homology with two B. lobbii species (KY966747.1 and KY966691.1) from China with a value of Per. Ident is 99.20%, while the ITS2 sequence has the highest homology with a Per. Ident value of 99.76% with the species B. lobbii (MG253848.1) from Poland. The results of the analysis show that the ITS2 sequence can identify species from the subspecies level or above (ordo or genus) and also improves good phylogenetic resolution in BLAST results, while the matK sequence makes little contribution in grouping the relationship between the B. lobbii species and other comparison species. The ITS2 sequence can be recommended as the best molecular marker for identifying Bulbophyllum orchid samples, especially Bulbophyllum lobbii.

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Section: Pendahuluanunclassified

Evaluasi Lokus Potensial matK dan ITS2 Untuk DNA Barcoding Anggrek Bulbophyllum lobbii Lindl.

Su'udi,

Ulum,

Ardiyansah

et al. 2024

Al-Kauniyah J. Biol.

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“…Batang berbentuk bundar tertutup daun, tinggi batang 3-7 m, Daun berbentuk jorong, tersusun rapat, berdaging, panjang 20-30 cm, lebar 7-12 cm, Bunga berbentuk pita, ujung runcing, panjang 50-100 cm, lebar 3 cm, lama mekar 5 hari, musim berbunga: Juni-Maret, daun kelopak berbentuk lanset, melancip, daun mahkota berbentuk bundar telur, panjang 5,5 cm dan lebar: 2,5 cm; Ekologi: anggrek tumbuh pada tempat terbuka pada ketinggian 550-1.600 m pdl (Wahyudiningsih et al, 2017).…”

Section: Hasil Dan Pembahasanunclassified

Eksplorasi Dan Identifi kasi Anggrek Terestrial Di Kawasan Gunung Jambu Jalur Pendakian Arah Lembang Kecamatan Tanjungsari Kabupaten Sumedang

2023

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Orchid is one of the ornamental plants that has a relatively high selling power and attractiveness, there are several types of orchids, including terrestrial orchids, this orchid is one of the orchids that grows and develops on the soil surface. its presence in the forest component and grows wildly, so if the forest component is disturbed it can threaten the sustainability of terrestrial orchids. Exploration and is one of the first steps in the existence of terrestrial orchids. This study aims to obtain data, study and obtain types of terrestrial orchids and vegetation in the Mount Jambu Area, Climbing Route to Lembang, Tanjungsari District, Sumedang Regency. This experiment was carried out from June to July 2021. The method used in this study was exploratory which was analyzed by descriptive method. The results of the study found 11 species, the orchid Nervilia punctata (BI.) Lindl., and Cymbidium lancifolium Hook. is an orchid that dominates in the Mount Jambu area of the hiking trail to Lembang and the vegetation in the area is a type of herbaceous plant that has narrow leaves and grows wild.

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“…Memiliki warna bunga bervariasi dilihat dari bentuk daun, bunga dan warna bunga. Anggrek ini memiliki warna bunga bervariasi berwarna putih hingga merah muda atau berwarna ungu terang, hal ini disebabkan penyesuaian tanaman tempat tumbuhnya (Wahyudiningsih et al, 2017). Saat pengamatan bunga ditemukan berwana putih dan bagian bibir ungu seperti terompet.…”

Section: Pembahasan Deskripsi Jenis Anggrekunclassified

Diversity of Orchids (Orchidaceae) in Bonjol Subdistrict, West Sumatra

Indriani,

Elfrida,

Pandia

2023

JBT

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Bonjol District, West Sumatra has a very high diversity of flora, one of the flora found in Bonjol District is orchids (Orchidaceae). However, there is no data that explains the diversity of orchid plants in Bonjol District, so researchers are interested in using this as research. This research aims to find out what types of orchids are found in Bonjol District, the diversity index of orchid plants (Orchidaceae) and the dominance index of the most dominant orchid plants in Bonjol District, West Sumatra. The research was carried out using an exploratory method with direct observation of orchid plants in the field. The sampling method used was purposive sampling, namely taking samples with certain considerations. Sampling was carried out at 2 stations, namely residential areas and forests. Data analysis used the Shannon-Wiener diversity index. The research results showed that the diversity of orchid species (Orchidaceae) in Bonjol District, West Sumatra was classified as moderate, with a total Shannon-Wiener diversity index H' = 1.4117. In Bonjol District, West Sumatra, there are 31 types of orchids from 17 genera, the most frequently found species are Bulbophyllum gibbosum with 35 individuals, while the species found least frequently are Nervelia crociformis and Eria discolor, each with 2 individuals. Then, in the results of calculating the dominance index value for orchids (Orchidaceae) in Bonjol District, West Sumatra, the result was D= 0.044, which means that no species dominates.

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Pemanfaatan Anggrek Spesies Kalimantan Tengah Berbasis Kearifan Lokal yang Berpotensi sebagai Bahan Obat Herbal

Abstract: Abstrak. Pemanfaatan anggrek spesies dari

Cited by 8 publications

References 12 publications

Evaluasi Lokus Potensial matK dan ITS2 Untuk DNA Barcoding Anggrek Bulbophyllum lobbii Lindl.

Evaluasi Lokus Potensial matK dan ITS2 Untuk DNA Barcoding Anggrek Bulbophyllum lobbii Lindl.

Eksplorasi Dan Identifi kasi Anggrek Terestrial Di Kawasan Gunung Jambu Jalur Pendakian Arah Lembang Kecamatan Tanjungsari Kabupaten Sumedang

Diversity of Orchids (Orchidaceae) in Bonjol Subdistrict, West Sumatra

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