Bioguided extraction of phenolic compounds and UHPLC-ESI-Q-TOF-MS/MS characterization of extracts of Moringa oleifera leaves collected in Brazil

Oldoni, Tatiane Luiza Cadorin; Merlin, Nathalie; Karling, Mariéli; Carpes, Solange Teresinha; Alencar, Severino Matias de; Morales, Rafael Gustavo Ferreira; Silva, Evandro; Pilau, Eduardo Jorge

doi:10.1016/j.foodres.2019.108647

Cited by 67 publications

(32 citation statements)

References 45 publications

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“…Antioxidant capacity and phenolic content of samples of plant extracts may vary as the result of several factors, such as the polarity of the solvent used for extraction, the extraction method and the material concentration. In addition, to measure antioxidant capacity of samples, there are several methods which are based on parameters of electron and hydrogen atom transfer (Oldoni et al, 2019). Therefore, FRAP (Ferric Reducing Antioxidant Power), DPPH (2,2-Diphenyl-1-picrylhydrazyl) and ABTS (2,2'-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) were the methods used for evaluating the antioxidant potential of EECC.…”

Section: Resultsmentioning

confidence: 99%

Ethanolic extract from Capsicum chinense Jacq. ripe fruits: phenolic compounds, antioxidant activity and development of biodegradable films

Santos¹,

Fernandes²,

Santos³

et al. 2021

Food Sci. Technol

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Capsicum spp. pepper has great economic and social importance in agribusiness worldwide, mainly associated with its high performance in cookery as a spice. This study aimed at determining the chemical profile of the ethanolic extract from Capsicum chinense Jacq. (EECC) ripe fruits by liquid chromatography-mass spectrometry (LC-MS) and at evaluating its antioxidant activity by DPPH, ABTS and FRAP. Besides, biodegradable films were prepared by incorporating EECC into arrowroot biofilms. LC-MS identified 10 phenolic compounds, a fact that corroborates its high concentration of total phenolic compounds, i. e., 277.62 ± 12.06 mg gallic acid/100g crude extract. High antioxidant activity of EECC was expressed as IC 50 values for reagents DPPH (IC 50 = 18.04 µg/mL), ABTS (IC 50 = 25.33 µg/mL) and FRAP (IC 50 = 128.58 µg/mL). Biodegradable films incorporating different doses of EECC (250-1000 µL) were obtained by a casting technique. The higher the EECC concentration, the higher their thickness, color, variable moisture content and the lower their solubility. This study proposes a new use to the plant extract from C. chinense, a natural product that may be applied to the development of biofilms to coat food and retard its deterioration. In addition, antioxidant activity of this type of pepper is also shown.

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Section: Resultsmentioning

confidence: 99%

Ethanolic extract from Capsicum chinense Jacq. ripe fruits: phenolic compounds, antioxidant activity and development of biodegradable films

Santos¹,

Fernandes²,

Santos³

et al. 2021

Food Sci. Technol

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“…Several studies reported different levels of these compounds in the members of the genus Astragalus [ 16 , 37 , 38 , 39 ]. Observed differences may be linked with geographical, environmental, and climatic conditions as well as plant parts [ 37 , 40 , 41 , 42 ]. In addition, recent studies indicated that the colorimetric methods had several drawbacks and these methods could not reflect accurate levels of these compounds in plant extracts [ 43 , 44 ].…”

Section: Resultsmentioning

confidence: 99%

Polyphenol Profile and Biological Activity Comparisons of Different Parts of Astragalus macrocephalus subsp. finitimus from Turkey

Sarıkürkçü

Zengin

2020

Biology

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The members of the genus Astragalus have great interest as traditional drugs in several folk systems including Turkey. In this sense, the present paper was aimed to explore the biological properties and chemical profiles of different parts (aerial parts, leaves, flowers, stems, and roots) of A. macrocephalus subsp. finitimus. Antioxidant (radical quenching, reducing power, and metal chelating) and enzyme inhibitory (α-amylase and tyrosinase) effects were investigated for biological properties. Regarding chemical profiles, individual phenolic compounds were detected by LC-MS, as well as total amounts. The leaves extract exhibited the strongest antioxidant abilities when compared with other parts. However, flowers extract had the best metal chelating ability. Hyperoside, apigenin, p-coumaric, and ferulic acids were identified as main compounds in the tested parts. Regarding enzyme inhibitory properties, tyrosinase inhibitory effects varied from IC50: 1.02 to 1.41 mg/mL. In addition, the best amylase inhibition effect was observed by leaves (3.36 mg/mL), followed by aerial parts, roots, stems, and flowers. As a result, from multivariate analysis, the tested parts were classified in three cluster. Summing up the results, it can be concluded that A. macrocephalus subsp. finitimus could be a precious source of natural bioactive agents in pharmaceutical, nutraceutical, and cosmeceutical applications.

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“…showed that the MOA α-L-rhamnosyl oligoglycoside isolated from M. oleifera leaves exerted cardioprotective effects (Panda et al, 2013;Cheraghi et al, 2017). MOA-trigonelline has been used to treat hyperglycemia, hyperlipidemia, insulin resistance, and diabetic auditory neuropathy (Oldoni et al, 2019); MOAbenzylamine decreased weight gain, fasting plasma glucose, and TC and increased glucose tolerance in insulin-resistant C57BL/6 mice (Iffiú-Soltész et al, 2010). However, the anti-tumor activity of MOA and their main active ingredients have not been characterized.…”

Section: Discussionmentioning

confidence: 99%

Moringa oleifera Alkaloids Inhibited PC3 Cells Growth and Migration Through the COX-2 Mediated Wnt/β-Catenin Signaling Pathway

Xie¹,

Luo²,

Shi³

et al. 2020

Front. Pharmacol.

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Moringa oleifera Lam. (M. oleifera) is valuable plant distributed in many tropical and subtropical countries. It has a number of medicinal uses and is highly nutritious. M. oleifera has been shown to inhibit tumor cell growth, but this effect has not been demonstrated on prostate cancer cells. In this study, we evaluated the inhibitory effect of M. oleifera alkaloids (MOA) on proliferation and migration of PC3 human prostate cancer cells in vitro and in vivo. Furthermore, we elucidated the mechanism of these effects. The results showed that MOA inhibited proliferation of PC3 cells and induced apoptosis and cell cycle arrest. Furthermore, MOA suppressed PC3 cell migration and inhibited the expression of matrix metalloproteinases (MMP)-9. In addition, MOA significantly downregulated the expression of cyclooxygenase 2 (COX-2), β-catenin, phosphorylated glycogen synthase 3β, and vascular endothelial growth factor, and suppressed production of prostaglandin E2 (PGE2). Furthermore, FH535 (β-catenin inhibitor) and MOA reversed PGE2-induced PC3 cell proliferation and migration, and the effects of MOA and FH535 were not additive. In vivo experiments showed that MOA (150 mg/kg) significantly inhibited growth of xenograft tumors in mice, and significantly reduced the protein expression levels of COX-2 and β-catenin in tumor tissues. These results indicate that MOA inhibits the proliferation and migration, and induces apoptosis and cell cycle arrest of PC3 cells. Additionally, MOA inhibits the proliferation and migration of PC3 cells through suppression of the COX-2 mediated Wnt/β-catenin signaling pathway.

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Bioguided extraction of phenolic compounds and UHPLC-ESI-Q-TOF-MS/MS characterization of extracts of Moringa oleifera leaves collected in Brazil

Cited by 67 publications

References 45 publications

Ethanolic extract from Capsicum chinense Jacq. ripe fruits: phenolic compounds, antioxidant activity and development of biodegradable films

Ethanolic extract from Capsicum chinense Jacq. ripe fruits: phenolic compounds, antioxidant activity and development of biodegradable films

Polyphenol Profile and Biological Activity Comparisons of Different Parts of Astragalus macrocephalus subsp. finitimus from Turkey

Moringa oleifera Alkaloids Inhibited PC3 Cells Growth and Migration Through the COX-2 Mediated Wnt/β-Catenin Signaling Pathway

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