“…The use of biota to investigate biogeographic regions, including their distribution patterns, is a key to better understand the ecological processes that create biotic differentiation and biodiversity at multiple spatiotemporal scales (Ricklefs, 2004). In particular, as an important step in understanding the spatial structure of biodiversity, delineating the distribution of these regions has been a foundation of basic and applied research in biogeography, ecology, earth science, and conservation ecology (Brum et al, 2017;Graham & Hijmans, 2006;Ibanez-Erquiaga, Pacheco, Rivadeneira, & Tejada, 2018;Kreft & Jetz, 2010;Lenormand et al, 2019;Olson et al, 2001;Ricklefs, 2004;Sun, Yan, & Xie, 2008). Until the late 20th century, the proposed biological regions were based on limited data, convenience, and expert opinions (White, 1983).…”