Biogenic synthesis of silver nanoparticles from Cassia fistula (Linn.): In vitro assessment of their antioxidant, antimicrobial and cytotoxic activities

Abstract

The present study reports on biogenic-synthesised silver nanoparticles (AgNPs) derived by treating Ag ions with an extract of leaf, a popular Indian medicinal plant found in natural habitation. The progress of biogenic synthesis was monitored time to time using a ultraviolet-visible spectroscopy. The effect of phytochemicals present in including flavonoids, tannins, phenolic compounds and alkaloids on the homogeneous growth of AgNPs was investigated by Fourier-transform infrared spectroscopy. The dynamic light scattering studies have revealed an average size and surface Zeta potential of the NPs as, -39.5 nm and -21.6 mV, respectively. The potential antibacterial and antifungal activities of the AgNPs were evaluated against and. Moreover, their strong antioxidant capability was determined by radical scavenging methods (1,1-diphenyl-2-picryl-hydrazil assay). Furthermore, the AgNPs displayed an effective cytotoxicity against A-431 skin cancer cell line by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, with the inhibitory concentration (IC) predicted as, 92.2 ± 1.2 μg/ml. The biogenically derived AgNPs could find immense scope as antimicrobial, antioxidant and anticancer agents apart from their potential use in chemical sensors and translational medicine.

Antimicrobial, Antioxidant and Cytotoxic Activity of Silver Nanoparticles Synthesized by Leaf Extract of Erythrina suberosa (Roxb.)

“…Current investigation result of antioxidant potential of E. suberosa offers an affirmative report toward hypothetical mechanism about involvement of antioxidant molecules from the leaf extract in the biogenic synthesis of silver nanoparticles. Previous research study revealed that, plant contains phenolic and flavonoids possess high antioxidant capabilities and hence biosynthesis of nanoparticles (Mohanta et al, 2016). …”

“…In the present study, we observed the appearance of absorption peak at ~428 nm. Previous study reported that AgNPs give absorption peak at 420–450 nm as a result of its surface plasmon resonance (SPR) character (Mohanta et al, 2016; Nayak et al, 2016). In our study, the observed absorption peak at 428 nm further confirms the biosynthesis of Ag nanoparticles (Figure 3).…”

“…Various chemical constituents/phyto-molecules have both protective and reductive activities which are mainly important for the reduction of silver ions adopting natural compounds and reductive enzyme complexes. In recent years, extracellular AgNPs were synthesized using different plant extracts as a potential reducing agent (Ahmed et al, 2016; Mohanta et al, 2016). …”

Biosynthesis of Silver Nanoparticles from Protium serratum and Investigation of their Potential Impacts on Food Safety and Control

“…Now a days, various plant parts like bark, leaf, fruit, stem and seed extracts have been successfully used for the synthesis of metal nanoparticles (Mittal et al, 2013). Among different metal nanoparticles, silver (Ag) nanoparticles have been used enormously due to their potential anti-bacterial (Mohanta et al, 2016a,b), anti-fungal and anti-proliferative activity (Kim et al, 2007; Nayak et al, 2015). Due to the excellent antimicrobial properties, the silver (Ag) nanoparticles have been extensively used in food packaging, food and seed preservation, biofertilizers, cosmetics and medicines (Marambio-Jones and Hoek, 2010; Dipankar and Murugan, 2012).…”

Characterization of Silver Nanoparticles Synthesizing Bacteria and Its Possible Use in Treatment of Multi Drug Resistant Isolate

“…Whereas, when the culture supernatant is treated with aqueous solution of silver nitrate, then it forms silver nanoparticles extracellularly [10]. In the recent past, silver nanoparticles have gainedattention of researchers worldwide due to their potential antimicrobial activities [8,[11][12][13]. The properties of silver nanoparticles have made them applicable in severalareassuch as biomedical, drug delivery, water treatment, agricultures, etc.…”