Biogenic synthesis, characterization of antibacterial silver nanoparticles and its cell cytotoxicity

Gopinath, V.; Priyadarshini, S.; Loke, Mun Fai; Arunkumar, J.; Marsili, Enrico; MubarakAli, D.; Velusamy, P.; Vadivelu, Jamuna

doi:10.1016/j.arabjc.2015.11.011

Cited by 173 publications

(97 citation statements)

References 42 publications

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“…Wherein cytotoxicity of P. putida synthesized silver nanoparticles against HEp-2 cells was determined by MTT assay. No significant cytotoxic effect at 25 μg/ml concentration was found, which was lethal for the bacteria tested for antibacterial efficacy (Gopinath et al, 2017). This study highlights and supports the results obtained for nanoparticles synthesized by P. hibiscicola although the range of concentration varies mainly due to the presence of protein in the nanomaterial.…”

Section: Discussionmentioning

confidence: 99%

Efficiency of Biosynthesized Silver and Zinc Nanoparticles Against Multi-Drug Resistant Pathogens

Punjabi

Mehta

Chavan³

et al. 2018

Front. Microbiol.

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Biosynthesis of metallic nanoparticles has acquired particular attention due to its economic feasibility, low toxicity, and simplicity of the process. In this study, extracellular synthesis of silver and zinc nanoparticle was carried out by Pseudomonas hibiscicola isolated from the effluent of an electroplating industry in Mumbai. Characterization studies revealed synthesis of 40 and 60 nm nanoparticles of silver (AgNP) and zinc (ZnNP), respectively, with distinct morphology as observed in TEM and its crystalline nature confirmed by XRD. DLS, zeta potential, NTA, and FTIR studies further characterized nanoparticles giving data about its size, stability, and functional groups. Considering the toxicity of nanoparticles the evaluation of antimicrobial activity was studied in the range of non-toxic concentration for normal cell lines. Silver nanoparticles were found to be the most effective antimicrobial against all tested strains and drug-resistant clinical isolates of MRSA, VRE, ESBL, MDR, Pseudomonas aeruginosa with MIC in the range of 1.25–5 mg/ml. Zinc nanoparticles were found to be specifically active against Gram-positive bacteria like Staphylococcus aureus including its drug-resistant variant MRSA. Both AgNP and ZnNP were found to be effective against Mycobacterium tuberculosis and its MDR strain with MIC of 1.25 mg/ml. The synergistic action of nanoparticles assessed in combination with a common antibiotic gentamicin (590 μg/mg) used for the treatment of various bacterial infections by Checker board assay. Silver nanoparticles profoundly exhibited synergistic antimicrobial activity against drug-resistant strains of MRSA, ESBL, VRE, and MDR P. aeruginosa while ZnNP were found to give synergism with gentamicin only against MRSA. The MRSA, ESBL, and P. aeruginosa strains exhibited MIC of 2.5 mg/ml except VRE which was 10 mg/ml for both AgNPs and ZnNPs. These results prove the great antimicrobial potential of AgNP and ZnNP against drug-resistant strains of community and hospital-acquired infections and opens a new arena of antimicrobials for treatment, supplementary prophylaxis, and prevention therapy.

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Section: Discussionmentioning

confidence: 99%

Efficiency of Biosynthesized Silver and Zinc Nanoparticles Against Multi-Drug Resistant Pathogens

Punjabi

Mehta

Chavan³

et al. 2018

Front. Microbiol.

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“…Viable cells are assumed to have intact and tight cell membranes that cannot be penetrated by some staining compounds, whereas dead cells are considered to have disrupted and/or broken membranes. 26,45 The combined usage of SYTO 9 and PI in a commercially available kit was first described in 1996, and it is promoted as a rapid and reliable method for assessment of bacterial viability that gives quantitative results and can be applied to microplate-reader flow-cytometry combined staining with SYTO 9 and PI.…”

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confidence: 99%

Photodynamic antimicrobial chemotherapy for <em>Staphylococcus aureus</em> and multidrug-resistant bacterial burn infection in vitro and in vivo

Mai¹,

Gao²,

Li³

et al. 2017

IJN

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Background and objectives Antibiotic resistance has emerged as one of the most important determinants of outcome in patients with serious infections, along with the virulence of the underlying pathogen. Photodynamic antimicrobial chemotherapy (PACT) has been proposed as an alternative approach for the inactivation of bacteria. This study aims to evaluate the antibacterial effect of sinoporphyrin sodium (DVDMS)-mediated PACT on Staphylococcus aureus and multidrug resistant S. aureus in vitro and in vivo. Materials and methods Bacteria were incubated with DVDMS and exposed to treatment with light. After PACT treatment, colony-forming units were counted to estimate the bactericidal effect. Intracellular reactive oxygen-species production was detected by flow cytometry. Flow cytometry and fluorescence-microscopy detection of bacterial cell-membrane permeability. Enzyme-linked immunosorbent assays were used to determine expression of VEGF, TGFβ 1 , TNFα, IL6, and bFGF factors in burn infection. Results DVDMS-PACT effectively killed bacterial proliferation. Intracellular ROS levels were enhanced obviously in the PACT-treatment group. SYTO 9 and propidium iodide staining showed a decrease in the ratio of green:red fluorescence intensity in the PACT-treatment group in comparison to the control group. Enzyme-linked immunosorbent-assay results revealed that in the healing process, the expression of bFGF, TGFβ 1 , and VEGF in the treatment group were higher than in the control group, which inhibited inflammation-factor secretion. In addition, skin-tissue bacteria were reduced after treatment. Conclusion These results indicate that DVDMS-PACT presents significant bactericidal activity and promotes wound healing after burn infections.

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“…For CS–DETBA, the obtained results demonstrated a strong inhibitory effect toward the tested strains of E. coli and P. aeruginosa at the 15 µg/mL MIC; this was less than that of S. aureus (21 µg/mL). The bacterial growth gradually decreased with increasing dosage of CS–DETBA to the bacteria . However, no growth inhibition was observed for the bacteria exposed to the control well.…”

Section: Resultsmentioning

confidence: 93%

Synthesis of a novel organosoluble, biocompatible, and antibacterial chitosan derivative for biomedical applications

Rizwan

Yahya

Hassan

et al. 2017

J of Applied Polymer Sci

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Electrospun materials have a number of applications in the tissue engineering field. However, the limited solubility of chitosan (CS), especially in organic solvents, makes its electrospinning with other synthetic organosoluble polymers impossible. In this article, we report the synthesis of a novel organosoluble derivative of CS through the application of a simple synthetic methodology. CS was reacted with 1,3‐diethyl‐2‐thiobarbituric acid (DETBA) with triethylorthoformate in the presence of methanol and acetic acid (4:1). The functional groups in the synthesized materials were confirmed by Fourier transform infrared and solid‐state NMR spectroscopy, whereas X‐ray diffraction revealed the level of crystallinity. The CS derivative (CS–DETBA) was tested for its cytotoxic effects on human gastric adenocarcinoma AGS cells and was found to be nontoxic. The prepared derivative showed a much enhanced inhibitory effect on the growth of three bacterial strains, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus, over that of CS itself. Overall, CS–DETBA showed good solubility in a range of organic solvents, such as dimethyl sulfoxide and N,N‐dimethylformamide, and was blended with polycaprolactone (PCL) to form films and electrospun nanofibers. The morphologies of the synthesized materials were analyzed by field emission scanning electron microscopy, and the fiber diameter was 360 nm under optimum conditions. This study demonstrated that the CS–DETBA–PCL blend could be a potential material for tissue engineering and biomedical applications. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 45905.

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Biogenic synthesis, characterization of antibacterial silver nanoparticles and its cell cytotoxicity

Cited by 173 publications

References 42 publications

Efficiency of Biosynthesized Silver and Zinc Nanoparticles Against Multi-Drug Resistant Pathogens

Efficiency of Biosynthesized Silver and Zinc Nanoparticles Against Multi-Drug Resistant Pathogens

Photodynamic antimicrobial chemotherapy for <em>Staphylococcus aureus</em> and multidrug-resistant bacterial burn infection in vitro and in vivo

Synthesis of a novel organosoluble, biocompatible, and antibacterial chitosan derivative for biomedical applications

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