Biogenic gold nanoparticles induce cell cycle arrest through oxidative stress and sensitize mitochondrial membranes in A549 lung cancer cells

Abstract

Schematic representation of biogenic synthesized AuNPs have been proven to have excellent anticancer activity against A549 human lung cancer cells.

Gold nanoparticle induces mitochondria-mediated apoptosis and cell cycle arrest in nonsmall cell lung cancer cells

“…DCFH-DA is a nonfluorescent dye and can diffuse into the cell through the plasma membrane where it is hydrolyzed to DCFH [21]. Nonfluorescent DCFH is finally converted to green fluorescent dichlorofluorescein (DCF) upon intracellular oxidation.…”

“…Cell death after treatment with AuNPs was quantified by using DAPI staining assay [21]. Cells were seeded in a six-well plate and grown for 24 h. After 24 h, the medium was replaced with fresh medium and treated with AuNPs and allowed to incubate for 24 h. The cells were stained with DAPI stain (2 μg/ ml) and incubated for 30 min.…”

“…Acridine orange/ethidium bromide double staining assay Acridine orange and ethidium bromide (AO/EB) staining method was carried out to detect morphological evidence of apoptosis in AuNP-treated cells [21]. Briefly, 5 × 10 5 cells were seeded on a cover slip in a six-well plate and allowed to attach overnight.…”

Development of glycolipid biosurfactant for inducing apoptosis in HeLa cells

“…After overnight incubation, cells were treated with glycolipids (10-200 µg/mL) dissolved in 0.1% of ethanol and Triton 100 (2-18 µg/ml). After 24 hr of incubation, 20 µl of MTT (5 mg/mL) was added and incubated for 4 hr, MTT was aspirated and 200 µl of DMSO was added to dissolve the formazan crystals 21 . The absorbance was measured at 570 nm using microplate reader (Biorad, USA).…”

“…ROS generation in both control and treated HeLa cells were measured using DCFH-DA staining method 21 . The cells were seeded in 6-well tissue culture plates (5×10 4 cell/mL).…”