Biogenesis of copper nanoparticles using peel extract ofPunica granatumand their antimicrobial activity against opportunistic pathogens

Abstract

Copper nanoparticles (CuNPs) were biologically synthesized using peel extract of Punica granatum as reducing agent as well as capping agent. On treatment of aqueous solutions of CuSO 4 •5H 2 O with peel extract of P. granatum, stable CuNPs were formed. UV-Visible spectrophotometer analysis confirmed the formation of CuNPs. The synthesized nanoparticles were characterized with Fourier transform infrared spectroscopy, particles size analyzer and transmission electron microscopy (TEM). The electron microscopy analysis of CuNPs indicated that they ranged in size from 15 to 20 nm. The biologically synthesized CuNPs demonstrated high antibacterial activity against opportunistic pathogens, that is, Micrococcus luteus MTCC 1809, Pseudomonas aeruginosa MTCC 424, Salmonella enterica MTCC 1253 and Enterobactor aerogenes MTCC 2823 in vitro. Nanoparticles synthesized biologically using plant extracts have the potential to serve as possible ecofriendly alternatives to chemical and physical methods for biomedical applications and research.! ARTICLE HISTORY

Green Adeptness in the Synthesis and Stabilization of Copper Nanoparticles: Catalytic, Antibacterial, Cytotoxicity, and Antioxidant Activities

“…The biogenic copper nanoparticles showed powerful antibacterial activity against gram-positive and gram-negative pathogens such as Pseudomonas aeruginosa (MTCC 424), Micrococcus luteus (MTCC 1809), Enterobacter aerogenes (MTCC 2832) [57], Salmonella enterica (MTCC 1253), Rhizoctonia solani , Xanthomonas axonopodis pv. citri , Xanthomonas axonopodis pv.…”

“…Copper nanoparticles were synthesized and stabilized in the literature by using different plants such as Euphorbia esula [56], Punica granatum [57], Ocimum sanctum [58], Ginkgo biloba [59], Calotropis procera [60], Lawsonia inermis [61], Citrus medicalinn [62], Camellia sinensis [63], Datura innoxia [64], Syzygium aromaticum [65], Sesamum indicum [66], Citrus limon , Turmeric curcumin [67], Gloriosa superba L. [68], Ficus carica [69], Aegle marmelos [70], Caesalpinia pulcherrima [71], Cassia fistula [72], Leucas aspera , Leucas chinensis [73], Delonix elata [74], Aloe barbadensis Miller [75], Thymus vulgaris [76], Phyllanthus emblica [77], Magnolia kobus [78], Eucalyptus [79], Artabotrys odoratissimus [80], Capparis zeylanica [81], Vitis vinifera [82], Hibiscus rosa-sinensis [83], Zingiber officinale [84], Datura metel [85], Zea mays [86], Urtica , Matricaria chamomilla , Glycyrrhiza glabra , Schisandra chinensis , Inula helenium , Cinnamomum [87], Dodonaea viscosa [88], Cassia auriculata [89], Azadirachta indica , Lantana camera , Tridax procumbens [90], Allium sativum [91], Asparagus adscendens , Bacopa monnieri , Ocimum bacilicum , Withania somnifera [92], Smithia sensitiva , Colocasia esculenta [93], Nerium oleander [94], and Psidium guajava [95]; by using different algae/fungi such as Phaeophyceae [96], Stereum hirsutum [97], and Hypocrea lixii [98]; and by using some microorganisms such as Pseudomonas fluorescens [99] and Enterococcus faecalis [100] cultures.…”

Management of wilt disease of chickpea in vivo by silver nanoparticles biosynthesized by rhizospheric microflora of chickpea (Cicer arietinum)

“…Green synthesis of nanoparticles has received attention due to its use of environment-friendly precursors for nanoparticles synthesis with many other advantages such as economic viability, ease of production, eco-friendly nature, avoiding the use of harmful chemicals, conditions of high temperature and pressure, and the expensive instruments required for physical and chemical methods. [1][2][3][4][5][6][7][8][9][10][11][12][13][14] Microorganisms have the ability to reduce heavy metal salts to metal nanoparticles with a narrow size distribution due to the presence of various reductase enzymes. 15 In a previous report, silver nanoparticles and PbS quantum dots were synthesized by Aspergillus sp.…”

Synthesis of CuS and ZnO/Zn(OH)2 nanoparticles and their evaluation for in vitro antibacterial and antifungal activities

“…Green chemistry utilizes nontoxic chemicals and environmentally friendly solvents for synthesizing NPs, which clearly shows a different path in eliminating wastes before it is generated. Nowadays, inorganic metal NPs such as MgO and ZnO, Ag, Au, Cu, Cu 2 O and Cu/Cu 2 O are increasingly used as antimicrobials owing to their stability and long shelf life compared with the organic antimicrobial agents. The exploration of nanostructures offers new possibilities in biological context, and owing to the fact that the surface area of particles increases by decreasing particle size, biological activity enhances per given mass in comparison with larger particles …”