Biogenesis and ceRNA role of circular RNAs in skeletal muscle myogenesis

Yue, Binglin; Wang, Jian; Song, Chengchuang; Cao, Xiukai; Huang, Yongzhen; Lan, Xianyong; Lei, Chuzhao; Huang, Bizhi

doi:10.1016/j.biocel.2019.105621

Cited by 14 publications

(12 citation statements)

References 71 publications

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“…Increasing evidence has suggested that ceRNA networks play key roles in a variety of biological processes, such as cancer [31,32], Alzheimer's disease [33], skeletal muscle myogenesis [34], and ageing [35,36]. Drosophila models are used to study multiple biological phenomena and various human diseases [7,[37][38][39][40].…”

Section: Discussionmentioning

confidence: 99%

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Age-related ceRNA networks and the mRNA/protein correlation in Drosophila ageing

Yang¹,

Yuán²,

He³

et al. 2021

Preprint

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Background As Drosophila is a classic model organism, understanding of the regulatory networks has great significance in revealing the genetic mechanisms of ageing and human diseases. Competing endogenous RNA (ceRNA)-mediated regulation is an important mechanism of circular RNA (circRNA) and long non-coding RNA (lncRNA) regulation in ageing and age-related disease. However, extensive analyses of the multi-omics (circRNA/miRNA/mRNA, lncRNA/miRNA/mRNA, and mRNA/protein) characteristics of adult Drosophila ageing have not been reported. Our results determine the ceRNA networks and the mRNA/protein correlations of key differentially expressed genes in adult Drosophila aging, and provide a solid foundation for understanding mechanisms of human ageing and ageing-related diseases. Results Here, differentially expressed circRNAs and microRNAs (miRNAs) between 7 and 42 day old Drosophila were screened and identified. Then, the differentially expressed mRNAs, circRNAs, miRNAs, lncRNAs, and proteins between day 7 and day 42 were used to analyse fly age-related circRNA/miRNA/mRNA and lncRNA/miRNA/mRNA networks and the mRNA/protein correlation in Drosophila ageing. Several key ceRNA networks were identified, such as the circ_0006913/miR-985-3p/Abl, circ_0009500/miR-14-5p/SERCA, XLOC_100429/miR-14-5p/SERCA, and XLOC_100429/miR-14-5p/Vha100-4 networks. Subsequently, 16 pairs of differentially expressed interacting mRNAs/proteins were found, including 10 pairs with the same expression trends and 6 pairs with the opposite expression trends. Furthermore, real-time quantitative PCR (qPCR) was used to verify the expression level of those genes. Conclusions The discovery of these ceRNA networks and the mRNA/protein correlations in adult Drosophila ageing will provide new information for research on human ageing and age-related disease.

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Section: Discussionmentioning

confidence: 99%

“…Furthermore, the ceRNA mechanism is a very important mechanism of circRNA and lncRNA regulation in the context of agerelated disease [43,54,55]. miRNAs are the core molecules of the ceRNA regulatory system [34,35]. Thus, screening of DE miRNAs is essential for research on ceRNA networks related to Drosophila ageing.…”

Section: Discussionmentioning

confidence: 99%

Age-related ceRNA networks and the mRNA/protein correlation in Drosophila ageing

Yang¹,

Yuán²,

He³

et al. 2021

Preprint

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“…Moreover, it was demonstrated that circRNAs are highly abundant in the mammalian brain compared to other tissues, which highlights the role of circRNAs in the CNS [ 42 ]. Recently, it has been reported that circRNAs are involved in skeletal muscle myogenesis [ 43 ] and, most importantly, that global circRNA levels are increased in DM1 [ 44 ]. Overall, until now, thousands of circRNAs that are expressed in animal cells have been identified and characterized, providing new insights regarding their biogenesis, the cell-type specificity of their expression, the extent to which they are conserved, the extent to which they are translated and their potential to act as miRNA sponges [ 45 ].…”

Section: A Brief Overview Of Ncrnas and Their Integrated Networkmentioning

confidence: 99%

“…As for circRNAs, numerous studies have also verified their gene regulatory functions in skeletal muscle development, including ceRNA mechanisms [ 39 , 43 , 115 , 116 , 117 ]. Together, recent findings on functions of lncRNAs and circRNAs in skeletal and cardiac muscles biology were extensively reviewed in [ 15 , 109 ], where their nuclear and cytoplasmic activities are also discussed, as well as potential crosstalk between miRNAs, circRNAs and lncRNAs.…”

Section: Current Understanding Of the Activity Of Cernas In Dms And Directions For Further Researchmentioning

confidence: 99%

Regulatory Potential of Competing Endogenous RNAs in Myotonic Dystrophies

Kościańska

Kozłowska

Fiszer

2021

IJMS

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Non-coding RNAs (ncRNAs) have been reported to be implicated in cell fate determination and various human diseases. All ncRNA molecules are emerging as key regulators of diverse cellular processes; however, little is known about the regulatory interaction among these various classes of RNAs. It has been proposed that the large-scale regulatory network across the whole transcriptome is mediated by competing endogenous RNA (ceRNA) activity attributed to both protein-coding and ncRNAs. ceRNAs are considered to be natural sponges of miRNAs that can influence the expression and availability of multiple miRNAs and, consequently, the global mRNA and protein levels. In this review, we summarize the current understanding of the role of ncRNAs in two neuromuscular diseases, myotonic dystrophy type 1 and 2 (DM1 and DM2), and the involvement of expanded CUG and CCUG repeat-containing transcripts in miRNA-mediated RNA crosstalk. More specifically, we discuss the possibility that long repeat tracts present in mutant transcripts can be potent miRNA sponges and may affect ceRNA crosstalk in these diseases. Moreover, we highlight practical information related to innovative disease modelling and studying RNA regulatory networks in cells. Extending knowledge of gene regulation by ncRNAs, and of complex regulatory ceRNA networks in DM1 and DM2, will help to address many questions pertinent to pathogenesis and treatment of these disorders; it may also help to better understand general rules of gene expression and to discover new rules of gene control.

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“…You are encouraged to use the Version of Record that, when published, will replace this version. The most up-to-date-version is available at https://doi.org/10.1042/BSR20202232 level of target gene mRNAs [19]. However, the mechanism underlying the regulatory role of ceRNAs in DCM remains unclear and needs further study.…”

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confidence: 99%

Long-chain noncoding RNA-GAS5/hsa-miR-138-5p attenuates high glucose-induced cardiomyocyte damage by targeting CYP11B2

et al. 2021

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Objective: Diabetic cardiomyopathy (DCM) is one of the complications experienced by patients with diabetes. In recent years, long noncoding RNAs (lncRNAs) have been investigated because of their role in the progression of various diseases, including DCM. The purpose of this study was to explore the role of lncRNA GAS5 in high-glucose (HG)-induced cardiomyocyte injury and apoptosis. Materials and methods: We constructed HG-induced AC16 cardiomyocytes and a streptozotocin-induced rat diabetes model. GAS5 was overexpressed and knocked out at the cellular level, and GAS5 was knocked down by lentiviruses at the animal level to observe its effect on myocardial injury. Real-time quantitative polymerase chain reaction was used to detect the expression of GAS5. Cell proliferation and apoptosis after GAS5 knockout were detected by CCK-8, TUNEL, and flow cytometry assays. ELISA was used to detect the changes in myocardial enzyme content in cells and animal myocardial tissues during the action of GAS5 on myocardial injury. Results: GAS5 expression was up-regulated in HG-treated AC16 cardiomyocytes and the rat diabetic myocardial injury model. The down-regulation of GAS5 inhibited HG-induced myocardial damage. This work proved that GAS5 konckdown reversed cardiomyocyte injury and apoptosis by targeting miR-138 to down-regulate CYP11B2. Conclusion: We confirmed for the first time that the down-regulation of GAS5 could reverse CYP11B2 via the miR-138 axis to reverse HG-induced cardiomyocyte injury. This research might provide a new direction for explaining the developmental mechanism of DCM and potential targets for the treatment of myocardial injury.

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Biogenesis and ceRNA role of circular RNAs in skeletal muscle myogenesis

Cited by 14 publications

References 71 publications

Age-related ceRNA networks and the mRNA/protein correlation in Drosophila ageing

Age-related ceRNA networks and the mRNA/protein correlation in Drosophila ageing

Regulatory Potential of Competing Endogenous RNAs in Myotonic Dystrophies

Long-chain noncoding RNA-GAS5/hsa-miR-138-5p attenuates high glucose-induced cardiomyocyte damage by targeting CYP11B2

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