Biogenesis and adhesion of type 1 and P pili

Lillington, James; Geibel, Sebastian; Waksman, Gabriel

doi:10.1016/j.bbagen.2014.04.021

Cited by 60 publications

(38 citation statements)

References 95 publications

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“…Type 1 and P-type fimbriae are major elements required for UPEC adhesion, invasion, and IBC formation (22). We therefore examined the effect of fur deletion on fimbria expression.…”

Section: Resultsmentioning

confidence: 99%

Fur Represses Adhesion to, Invasion of, and Intracellular Bacterial Community Formation within Bladder Epithelial Cells and Motility in Uropathogenic Escherichia coli

et al. 2016

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Uropathogenic Escherichia coli (UPEC) is a major pathogen that causes urinary tract infections (UTIs). This bacterium adheres to and invades the host cells in the bladder, where it forms biofilm-like polymicrobial structures termed intracellular bacterial communities (IBCs) that protect UPEC from antimicrobial agents and the host immune systems. Using genetic screening, we found that deletion of the fur gene, which encodes an iron-binding transcriptional repressor for iron uptake systems, elevated the expression of type I fimbriae and motility when UPEC was grown under iron-rich conditions, and it led to an increased number of UPEC cells adhering to and internalized in bladder epithelial cells. Consequently, the IBC colonies that the fur mutant formed in host cells were denser and larger than those formed by the wild-type parent strain. Fur is inactivated under iron-restricted conditions. When iron was depleted from the bacterial cultures, wild-type UPEC adhesion, invasion, and motility increased, similar to the case with the fur mutant. The purified Fur protein bound to regions upstream of fimA and flhD, which encode type I fimbriae and an activator of flagellar expression that contributes to motility, respectively. These results suggest that Fur is a repressor of fimA and flhD and that its repression is abolished under iron-depleted conditions. Based on our in vitro experiments, we conclude that UPEC adhesion, invasion, IBC formation, and motility are suppressed by Fur under iron-rich conditions but derepressed under iron-restricted conditions, such as in patients with UTIs.

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“…Type 1 and P-type fimbriae are major elements required for UPEC adhesion, invasion, and IBC formation (22). We therefore examined the effect of fur deletion on fimbria expression.…”

Section: Resultsmentioning

confidence: 99%

Fur Represses Adhesion to, Invasion of, and Intracellular Bacterial Community Formation within Bladder Epithelial Cells and Motility in Uropathogenic Escherichia coli

et al. 2016

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“…The tip of these pili is constituted by an adhesion factor called PapG, that binds to glycosphingolipids of the kidney epithelium [32]. Some UPEC strains also possess Type I pili at their surface, which have binding specificity to D-mannosylated receptors, such as the uroplakins of the bladder [33]. Type IV pili constitute another class of polymeric adhesive surface structures expressed by different gram-negative bacteria [34].…”

Section: Mechanisms Of Bacterial Adhesion To Host Cellsmentioning

confidence: 99%

How bacterial pathogens colonize their hosts and invade deeper tissues

Ribet

Cossart

2015

Microbes and Infection

587

404

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Bacterial pathogens have evolved a wide range of strategies to colonize and invade human organs, despite the presence of multiple host defense mechanisms. In this review, we will describe how pathogenic bacteria can adhere and multiply at the surface of host cells, how some bacteria can enter and proliferate inside these cells, and finally how pathogens may cross epithelial or endothelial host barriers and get access to internal tissues, leading to severe diseases in humans.

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“…The biogenesis and adhesion properties of bacterial pili have been extensively reviewed by Lillington et al (2014) [17].…”

Section: Discussionmentioning

confidence: 99%

How methylglyoxal kills bacteria: An ultrastructural study

Rabie

Serem

Oberholzer

et al. 2016

Ultrastructural Pathology

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Antibacterial activity of honey is due to the presence of methylglyoxal (MGO), H 2 O 2 , bee defensin as well as polyphenols. High MGO levels in manuka honey are the main source of antibacterial activity. Manuka honey has been reported to reduce the swarming and swimming motility of Pseudomonas aeruginosa due to de-flagellation. Due to the complexity of honey it is unknown if this effect is directly due to MGO. In this ultrastructural investigation the effects of MGO on the morphology of bacteria and specifically the structure of fimbriae and flagella were and E. coli morphology was then evaluated. At 0.5 mM MGO, bacteria structure was unaltered.For both bacteria at 1 mM MGO fewer fimbriae were present and the flagella were less or absent. Identified structures appeared stunted and fragile. At 2 mM MGO fimbriae and flagella were absent while the bacteria were rounded with shrinkage and loss of membrane integrity.Antibacterial MGO causes alterations in the structure of bacterial fimbriae and flagella which would limit bacteria adherence and motility.

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Biogenesis and adhesion of type 1 and P pili

Cited by 60 publications

References 95 publications

Fur Represses Adhesion to, Invasion of, and Intracellular Bacterial Community Formation within Bladder Epithelial Cells and Motility in Uropathogenic Escherichia coli

Fur Represses Adhesion to, Invasion of, and Intracellular Bacterial Community Formation within Bladder Epithelial Cells and Motility in Uropathogenic Escherichia coli

How bacterial pathogens colonize their hosts and invade deeper tissues

How methylglyoxal kills bacteria: An ultrastructural study

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