Experimental work was carried out for the production of Biogas from poultry waste water. The Poultry waste was collected from farm near Nagercoil at Kanyakumari District. Batch anaerobic digester was designed for 20L capacity. The experiment was carried out for 36 days to monitor the performance. Various parameters like pH, TS, COD have checked for every 24hours. The Production of biogas was measured by water displacement method. The methane content was analyzed by gas chromatography test. Based on the experimental data, kinetics studies have done for various models like Line Weaver-Burk method, Eadie-Hofstee method, Hanes-Woolf method. The Eadie-Hofstee Method has provided better prediction than other method. These results thus indicate that, Eadie-Hofstee Method is best to identify the growth rate, substrate concentration and Limiting Substrate Concentration of the system. The sludge of the poultry wastewater and digester were characterized by SEM analysis. The imaging was done to determine the morphological structure of the sludge and to view the bacterial growth on the surface of the sludge.