Biogas production and valorization by means of a two-step biological process

Converti, Attílio; Oliveira, Ricardo Pinheiro de Souza; Torres, Beatriz Rivas; Lodi, Alessandra; Zilli, Mario

doi:10.1016/j.biortech.2009.05.072

Cited by 123 publications

(47 citation statements)

References 29 publications

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“…In this context, Posadas et al [10] recorded CO 2 removals from synthetic biogas of 99% and O 2 concentrations in the upgraded biogas of ≈20% in a 180 L open photobioreactor treating diluted centrates, while Serejo et al [12] recorded CO 2 and H 2 S removals of ≈ 80% and 100%, respectively, and O 2 concentrations in the upgraded stream ranging from 2 ± 1% to 1 ± 0% in a similar photobioreactor by treating diluted anaerobically digested vinasse (ADV). Likewise, Converti et al [13] reported O 2 concentrations ranging from 10 to 24% during the upgrading of real biogas in a bubble column photobioreactor. These high O 2 levels entail a potential explosion hazard and prevent the injection of the upgraded biogas into natural gas networks.…”

Section: Introductionmentioning

confidence: 96%

Minimization of biomethane oxygen concentration during biogas upgrading in algal–bacterial photobioreactors

et al. 2015

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Section: Introductionmentioning

confidence: 96%

Minimization of biomethane oxygen concentration during biogas upgrading in algal–bacterial photobioreactors

et al. 2015

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“…The culture was subjected to a diel rhythm of 12 h of light and 12 h of darkness (LD), which resulted in rapid oxygen depletion during the dark period. These are conditions that mimic the conditions of mass culturing in a large-scale closed photobioreactor, equipped with a degassing system and sparged with off-gases originating from the combustion of fossil fuel (18) or an anaerobic digester (19). To achieve high cell densities, high light intensities are needed which, depending on geographical location, may regularly exceed the photosynthetic capacity of cells (especially at midday in full sunlight).…”

mentioning

confidence: 99%

Culturing Synechocystis sp. Strain PCC 6803 with N ₂ and CO ₂ in a Diel Regime Reveals Multiphase Glycogen Dynamics with Low Maintenance Costs

Angermayr

Alphen

Hasdemir

et al. 2016

Appl Environ Microbiol

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Investigating the physiology of cyanobacteria cultured under a diel light regime is relevant for a better understanding of the resulting growth characteristics and for specific biotechnological applications that are foreseen for these photosynthetic organisms. Here, we present the results of a multiomics study of the model cyanobacterium Synechocystis sp. strain PCC 6803, cultured in a lab-scale photobioreactor in physiological conditions relevant for large-scale culturing. The culture was sparged with N 2 and CO 2 , leading to an anoxic environment during the dark period. Growth followed the availability of light. Metabolite analysis performed with 1 H nuclear magnetic resonance analysis showed that amino acids involved in nitrogen and sulfur assimilation showed elevated levels in the light. Most protein levels, analyzed through mass spectrometry, remained rather stable. However, several high-light-response proteins and stress-response proteins showed distinct changes at the onset of the light period. Microarray-based transcript analysis found common patterns of ϳ56% of the transcriptome following the diel regime. These oscillating transcripts could be grouped coarsely into genes that were upregulated and downregulated in the dark period. The accumulated glycogen was degraded in the anaerobic environment in the dark. A small part was degraded gradually, reflecting basic maintenance requirements of the cells in darkness. Surprisingly, the largest part was degraded rapidly in a short time span at the end of the dark period. This degradation could allow rapid formation of metabolic intermediates at the end of the dark period, preparing the cells for the resumption of growth at the start of the light period. IMPORTANCEIndustrial-scale biotechnological applications are anticipated for cyanobacteria. We simulated large-scale high-cell-density culturing of Synechocystis sp. PCC 6803 under a diel light regime in a lab-scale photobioreactor. In BG-11 medium, Synechocystis grew only in the light. Metabolite analysis grouped the collected samples according to the light and dark conditions. Proteome analysis suggested that the majority of enzyme-activity regulation was not hierarchical but rather occurred through enzyme activity regulation. An abrupt light-on condition induced high-light-stress proteins. Transcript analysis showed distinct patterns for the light and dark periods. Glycogen gradually accumulated in the light and was rapidly consumed in the last quarter of the dark period. This suggests that the circadian clock primed the cellular machinery for immediate resumption of growth in the light. Understanding cyanobacterial physiology in a diel environment is of interest to understand circadian regulation in general and for the utilization of these organisms in biotechnological applications. Our exploration of the effect of a diel light cycle on a cyanobacterial culture started with the wish to investigate the response of the metabolic network of the cells to the imposed repetitively fluctuating environment,...

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“…Anaerobic digestion of biomass produces an effluent, which contains important nutrients such as carbon, nitrogen and phosphorus, and these can be recovered for the production of microalgal biomass (Converti et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Spirulina sp. LEB-18 culture using effluent from the anaerobic digestion

Borges¹,

Rosa²,

Meza³

et al. 2013

Braz. J. Chem. Eng.

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-The carbon source is the most expensive nutrient for Spirulina production; effluents from anaerobic digestion contain this nutrient in the form of HCO 3 -. The aim of this study was to assess the growth kinetics, composition and fatty acid profile of Spirulina sp. LEB-18 grown in standard Zarrouk medium (NaHCO 3 16.8 g L -1) and in Zarrouk medium replaced with 20% (v/v) effluent with reduced concentrations of NaHCO 3 (5.3 and 2.8 g L -1 ). The use of effluent and lower concentrations of HCO 3 -was found to be an alternative to reduce the costs of Spirulina production, because there were no significant differences in growth parameters (μ max 0.324 -0.354 d -1 ; P max 0.280 -0.297 g L -1 d -1 ), in the different culture medium used. Lipids ranged between 4.9 and 5.0%; the media with effluent had higher levels of linoleic acid compared to the standard medium.

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Biogas production and valorization by means of a two-step biological process

Cited by 123 publications

References 29 publications

Minimization of biomethane oxygen concentration during biogas upgrading in algal–bacterial photobioreactors

Minimization of biomethane oxygen concentration during biogas upgrading in algal–bacterial photobioreactors

Culturing Synechocystis sp. Strain PCC 6803 with N ₂ and CO ₂ in a Diel Regime Reveals Multiphase Glycogen Dynamics with Low Maintenance Costs

Spirulina sp. LEB-18 culture using effluent from the anaerobic digestion

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Biogas production and valorization by means of a two-step biological process

Cited by 123 publications

References 29 publications

Minimization of biomethane oxygen concentration during biogas upgrading in algal–bacterial photobioreactors

Minimization of biomethane oxygen concentration during biogas upgrading in algal–bacterial photobioreactors

Culturing Synechocystis sp. Strain PCC 6803 with N 2 and CO 2 in a Diel Regime Reveals Multiphase Glycogen Dynamics with Low Maintenance Costs

Spirulina sp. LEB-18 culture using effluent from the anaerobic digestion

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Culturing Synechocystis sp. Strain PCC 6803 with N ₂ and CO ₂ in a Diel Regime Reveals Multiphase Glycogen Dynamics with Low Maintenance Costs