“…A modification of earlier methods [12,15,40] was used in this design, the value employed for the various variables were: (i) Exposure time (55,65,75, 85 and 95 min) (ii) shaker temperature (20, 25, 30, 35 and 40 C), (iii) Agitation (115, 125, 135, 145 and 155 rpm), (iv) Dry mass (3, 4.5, 6, 7.5 and 9 g) and (v) H 2 O 2 concentration (2,4,6,8 and 10% (v.v À1 )). In order to reduce the incidence of foaming due to the presence of hydrogen peroxide, 0.5 mL of antifoam agent (Biocane FC 500, Brazil) was added to the setup.…”