Biofunctionalization of Fluorescent Nanoparticles

Abstract

The sections in this article are Introduction Fluorescent Nanoparticle Probes Dye‐doped Nanoparticles Quantum Dots ( QDs ) Metal Nanoparticles Hybrid Architectures Involving Fluorescent Nanoprobes Metal–Dye Dye‐doped Silica Shells Quantum Dot‐containing Microspheres Bioconjugation of Fluorescent Nanoparticles General Considerations Overview Common Coupling Reactions Bioconjugation of Polymeric Nanoparticles Noncovalent Approaches Covalent Approaches Bioconjugation of Quantum Dots Noncovalent Approaches Covalent Approaches Bioconjugation of Metallic Nanoprobes Noncovalent Approaches Covalent Approaches Design of Biocompatible Coatings General Considerations Overview Colloidal Stability Biocompatible Surfaces Cytotoxicity Nanoparticle‐stabilizing Coatings Low Cytotoxicity Coatings Applications Biosensing Polymeric Sensors Quantum Dot Sensors Metallic Sensors Fluorescent Nanoparticles as Labels in Biological Imaging Dye‐doped Nanoparticles Quantum Dots

Fluorescent monolayer protected gold nanoparticles – Preparation and structure elucidation

“…Noble metal nanoparticles modified with organic fluorophores are of particular importance for fabrication of new fluorescent materials for bio-and chemical sensing [1,4,5]. For a long period, it was accepted that gold and silver nanoparticles strongly quench the emission of fluorophores attached to their surface through an energy-transfer mechanism [6][7][8][9].…”

Luminescent Quantum Dot FRET‐Based Probes in Cellular and Biological Assays

“…More specifically, we focus on studies carried out in our laboratory toward the development of quantum dot fluorescence resonance energy transfer (FRET)-based protease sensors for real-time monitoring of proteolytic activity as a mean to discriminate between normal and cancerous cells. These studies are a part of a large effort by the research community to develop sensors with high sensitivity and high specificity for cancer research [1]. To date, fluorescence methods based on the use of molecular fluorophores have been the most common methods of detecting biomolecules.…”

Highly luminescent nanostructures of CdS and ZnS prepared by microwaves heating: effect of sulphide concentration

“…As the result of this condition, the state of free charge carriers within a nanocrystal is quantized and the spacing of the discrete energy states (emission colors) is linked to their size of nanoparticle. The combination of small size, high photostability, and size-tunable emission properties makes quantum dots highly attractive probes for biological, biomedical, and bioanalytical imaging applications [48][49][50][51].…”