Biofuel fromD-xylose — The second most abundant sugar

Lachke, Anil

doi:10.1007/bf02836736

Cited by 47 publications

(27 citation statements)

References 2 publications

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“…However, Zymomonas mobilis has been the object of considerable interest in the past decades because of its ability to ferment hexoses into ethanol rapidly and efficiently [3]. Despite this, wild Z. mobilis strains cannot use xylose, the major component of hemicellulose [4]. Hence, many efforts have been devoted to develop efficient xylose-fermenting Z. mobilis, and at present, several recombinant strains capable of fermenting xylose to ethanol have been engineered [5,6].…”

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Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology

Dong

Zhao

et al. 2012

Chin. Sci. Bull.

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A synthetic medium for ethanol production by recombinant xylose-fermenting Zymomonas mobilis was optimized using Plackett-Burman (PB) design and response surface methodology (RSM). The effects of 19 medium components were investigated by PB design. Eight of these components were determined to have significant effects on ethanol production. The statistical model was constructed via central composite design (CCD) using five selected variables, including xylose, trisodium citrate, choline chloride, pyridoxine, and thiamine. The validity of the developed model was verified. The ethanol concentration in the optimized medium was 6.7% higher than in the RM medium. The optimized medium was then simplified to give the final synthetic medium (S2), in which the ethanol concentration was 20.7% higher than in the RM medium. Xylose and trisodium citrate were the key medium components influencing ethanol production, while calcium pantothenate was the only growth factor required by recombinant Z. mobilis. Ethanol production and growth response were directly proportional to the logarithm of the concentration of trisodium citrate in the range of 0.1-1.6 g/L.recombinant Zymomonas mobilis, synthetic medium, ethanol production, response surface methodology, trisodium citrate, xylose Citation:Dong H N, Zhao X M, Ma Y Y, et al. Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology.

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Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology

Dong

Zhao

et al. 2012

Chin. Sci. Bull.

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“…Además, como no es metabolizado por las bacterias que provocan caries, puede ser utilizado como endulzante no cariogénico en las pastas de dientes (Emodi, 1978, Mäkinen, 1979, Aguirre-Zero et al, 1993, Nothenberg, 1994. Existen en la literatura referencias sobre el uso industrial de la xilosa, tales como la producción de etanol, xilitol, penicilina, ácido SOMENTA CIENCIA Y TECNOLOGÍA ALIMENTARIA Sociedad Mexicana de Nutrición y Tecnología de los Alimentos acético y láctico, polímeros de plásticos biodegradables, pegamentos, resinas y levadura cervecera, entre otras (Lachke, 2002;www.avantrex.com/resorces/sweet2003-4.html).…”

Section: Introductionunclassified

Purificación De Hidrolizado De Bagazo De Caña De Azúcar Con Carbón Activado Y Resinas De Intercambio Iónico Purification of Sugarcane Bagasse Hydrolysates Using Activated Charcoal and Ion-Exchange Resins

Solenzal

Aroche

Verde

et al. 2006

Ciencia y Tecnologia Alimentaria

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“…Additionally, the arabinose side chain is further modified by phenolic compounds such as ferulic acid, which provides cross-links with other xylan chains or lignin. Recent interest in using xylan as feedstock for biofuels and bioproducts has led to the development of numerous promising processes for a variety of products, such as biodiesel (5,26), xylitol (10,12,18,21), and ethanol (7,9,11,13). Although xylan is relatively easily hydrolyzed through chemical pretreatment, the process often generates inhibitors that compromise the subsequent bioconversion catalyzed by biocatalysts (23,28).…”

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confidence: 99%

Escherichia coliBinary Culture Engineered for Direct Fermentation of Hemicellulose to a Biofuel

Shin

McClendon

et al. 2010

Appl Environ Microbiol

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Metabolic engineering has created several Escherichia coli biocatalysts for production of biofuels and other useful molecules. However, the inability of these biocatalysts to directly use polymeric substrates necessitates costly pretreatment and enzymatic hydrolysis prior to fermentation. Consolidated bioprocessing has the potential to simplify the process by combining enzyme production, hydrolysis, and fermentation into a single step but requires a fermenting organism to multitask by producing both necessary enzymes and target molecules. We demonstrate here a binary strategy for consolidated bioprocessing of xylan, a complex substrate requiring six hemicellulases for complete hydrolysis. An integrated modular approach was used to design the two strains to function cooperatively in the process of transforming xylan into ethanol. The first strain was engineered to coexpress two hemicellulases. Recombinant enzymes were secreted to the growth medium by a method of lpp deletion with over 90% efficiency. Secreted enzymes hydrolyzed xylan into xylooligosaccharides, which were taken in by the second strain, designed to use the xylooligosaccharides for ethanol production. Cocultivation of the two strains converted xylan hemicellulose to ethanol with a yield about 55% of the theoretical value. Inclusion of other three hemicellulases improved the ethanol yield to 70%. Analysis of the culture broth showed that xylooligosaccharides with four or more xylose units were not utilized, suggesting that improving the use of higher xyloogligomers should be the focus in future efforts. This is the first demonstration of an engineered binary culture for consolidated bioprocessing of xylan. The modular design should allow the strategy to be adopted for a broad range of biofuel and biorefinery products.

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Biofuel fromD-xylose — The second most abundant sugar

Cited by 47 publications

References 2 publications

Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology

Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology

Purificación De Hidrolizado De Bagazo De Caña De Azúcar Con Carbón Activado Y Resinas De Intercambio Iónico Purification of Sugarcane Bagasse Hydrolysates Using Activated Charcoal and Ion-Exchange Resins

Escherichia coliBinary Culture Engineered for Direct Fermentation of Hemicellulose to a Biofuel

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