Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology

Abstract

A synthetic medium for ethanol production by recombinant xylose-fermenting Zymomonas mobilis was optimized using Plackett-Burman (PB) design and response surface methodology (RSM). The effects of 19 medium components were investigated by PB design. Eight of these components were determined to have significant effects on ethanol production. The statistical model was constructed via central composite design (CCD) using five selected variables, including xylose, trisodium citrate, choline chloride, pyridoxine, and thiamine. The validity of the developed model was verified. The ethanol concentration in the optimized medium was 6.7% higher than in the RM medium. The optimized medium was then simplified to give the final synthetic medium (S2), in which the ethanol concentration was 20.7% higher than in the RM medium. Xylose and trisodium citrate were the key medium components influencing ethanol production, while calcium pantothenate was the only growth factor required by recombinant Z. mobilis. Ethanol production and growth response were directly proportional to the logarithm of the concentration of trisodium citrate in the range of 0.1-1.6 g/L.recombinant Zymomonas mobilis, synthetic medium, ethanol production, response surface methodology, trisodium citrate, xylose Citation:Dong H N, Zhao X M, Ma Y Y, et al. Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology.

Ethanol production from sweet sorghum by Saccharomyces cerevisiae DBKKUY-53 immobilized on alginate-loofah matrices

“…This might be due to the negative effects of high concentrations of sugar and ethanol on growth and metabolic processes in yeast cells. 15 By the way, the results of this study demonstrate that response surface methodology based on the CCD model is a powerful tool to determine the optimum values of the individual variables and the maximum response values as also described by Dong et al 33

Fig. 2 Verification experiments of ethanol production from sweet sorghum juice using the ALM-immobilized cells of S. cerevisiae DBKKUY-53.

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Dynamics of ethanol production from deproteinized whey by Kluyveromyces marxianus: An analysis about buffering capacity,thermal and nitrogen tolerance

“…4, Table 2) seemed to be due to inherent characteristics of yeast extract (YE), which contained thiamine (Tomaszewska et al 2014). Interestingly, thiamine has been described as a key component of a fermentation medium that affected ethanol production, and maximized ethanol production in an optimized medium (Dong et al 2012). Similarly, when compared to total nitrogen consumption (Fig.…”

A newly isolated and identified vitamin B12 producing strain: Sinorhizobium meliloti 320

“…Response surface methodology (RSM)-central composite design (CCD) was employed to find out the optimum concentrations of the optimal carbon source and nitrogen source and to study their interactions [24]. Experimental design, model calculation, graph drawing and other data analysis were all performed on Design Expert software (Version 8.0.6, Stat-Ease Inc., Minneapolis, USA).…”