Biofluids manipulation methods for liquid biopsy in minimally-invasive assays

Miniaturized Real-Time PCR systems for SARS-CoV-2 detection at the Point-of-Care

“…For these reasons, the prevention, control and prompt diagnosis are complex but crucial in the battle against the spread of COVID-19 [12] . A key point is self-diagnosis which can be done only if the biological fluids are easily collected and the test is simple and straightforward [13] .…”

Development of an MIP based electrochemical sensor for TGF-β1 detection and its application in liquid biopsy

“…The use of artificial receptors based on MIP as an alternative to antibodies has been exploited here for the first time in a saliva sample without requiring any labeling steps and with only simple pre-treatment. 28,29 For these reasons, the developed sensor is suitable for point of care diagnostics since it allows very fast and simple analysis in a single step. The integration of MIPs into miniaturized devices, combined with their long shelf-life and no need for refrigerated storage, makes them innovative and powerful tools for developing easy and portable devices for liquid biopsy with the aim to increase the life expectancy of patients.…”

Challenges and Opportunities for Extracellular Vesicles in Clinical Oncology Therapy

“…In recent years, EVs have been found in a variety of physiological fluids including blood, urine, saliva, breast milk, amniotic fluid, ascites, cerebrospinal fluid, bile, and semen [ 8 , 9 , 10 , 11 , 12 ]. More importantly, EVs can be directly isolated from physiological fluid in solid organs including the liver, heart, brain, and others [ 13 , 14 , 15 , 16 , 17 ].…”