Development of an MIP based electrochemical sensor for TGF-β1 detection and its application in liquid biopsy

Siciliano, Giulia; Chiriaco, Maria; Ferrara, Francesco; Turco, A.; Velardi, L.; Signore, M.A.; Esposito, Marco; Gigli, Giuseppe; Primiceri, Elisabetta

doi:10.1039/d3an00958k

Cited by 9 publications

(4 citation statements)

References 40 publications

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“…Saliva, rich in molecular information, presents a unique opportunity for the development of targeted biosensors. For instance, the use of molecularly imprinted polymers for the detection of transforming growth factor‐beta‐1 (TGF‐β1) is an example of how advances in biosensor technology are tapping into salivary diagnostics, paving the way for noninvasive, point‐of‐care cancer screening and monitoring 75 …”

Section: Clinical Applications and Case Studies In Diverse Fluidsmentioning

confidence: 99%

Beyond blood: Advancing the frontiers of liquid biopsy in oncology and personalized medicine

Bao,

Zhang,

Guo

et al. 2024

Cancer Science

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Liquid biopsy is emerging as a pivotal tool in precision oncology, offering a noninvasive and comprehensive approach to cancer diagnostics and management. By harnessing biofluids such as blood, urine, saliva, cerebrospinal fluid, and pleural effusions, this technique profiles key biomarkers including circulating tumor DNA, circulating tumor cells, microRNAs, and extracellular vesicles. This review discusses the extended scope of liquid biopsy, highlighting its indispensable role in enhancing patient outcomes through early detection, continuous monitoring, and tailored therapy. While the advantages are notable, we also address the challenges, emphasizing the necessity for precision, cost‐effectiveness, and standardized methodologies in its broader application. The future trajectory of liquid biopsy is set to expand its reach in personalized medicine, fueled by technological advancements and collaborative research.

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Section: Clinical Applications and Case Studies In Diverse Fluidsmentioning

confidence: 99%

Beyond blood: Advancing the frontiers of liquid biopsy in oncology and personalized medicine

Bao,

Zhang,

Guo

et al. 2024

Cancer Science

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“…The sensor could distinguish one and three mismatched nucleotides in target miRNA, demonstrating high selectivity. Siciliano et al [36] developed a molecularly imprinted polymer (MIP)-based electrochemical sensor for TGF-β1 detection and its application in liquid biopsy. Using TGF-β1 as the template molecule, a biomimetic surface was synthesized via the polymerization of aniline monomers on a platinum electrode, followed by detection using differential pulse voltammetry.…”

Section: Development Of Biochemical Sensors For Oral Cancer Markersmentioning

confidence: 99%

“…(b) The nanocomposite of AuNPs@ZIF-8/Cu-based construction process of a label-free electrochemical sensor and DNA strand displacement reaction for ORAOV 1 detection [37]. (c) Fabrication of TGF-β1 MIP on primary platinum microelectrodes patterned on glass substrate receptors and detection of TGF-β1 in saliva samples through the fabricated MIP electrochemical sensor [36]. (d) Schematic diagram of Cyfra21-1 detection based on AuNPs@HRP@FeMOF immunoscaffold and FASA [40].…”

Section: Development Of Biochemical Sensors For Oral Cancer Markersmentioning

confidence: 99%

Application of Sensing Devices in the Detection of Oral, Pulmonary, and Gastrointestinal Diseases

Yu,

Mou,

Zhang

et al. 2024

Chemosensors

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Biomedical sensing technology is developing at a tremendous pace and is expected to become an effective clinical tool for the diagnosis and monitoring of human health. The development of sensing devices has successfully transformed the specific sensor prototype designed in the laboratory into a commercially feasible clinical disease detection device. Recently, sensing devices have been accelerated and extended to various fields beyond disease detection, including the measurement of gastrointestinal physiological parameters such as pH, VOC detection, small-molecule gas sensing, and noninvasive screening of oral and lung diseases such as oral cancer, gastric cancer, and other major diseases. In this review, the applications of sensors and electronic nose devices in the diagnosis and monitoring of oral, pulmonary, and gastrointestinal diseases are reviewed, as well as the design and application of sensor materials in disease markers and in situ detection. This article also introduces the practical application of sensing devices in human disease detection, critically analyzes their detection mechanisms and clinical utility, and discusses their future development in medicine. We believe that this review will help readers, especially practitioners in the medical field, provide ideas for the development of sensing devices.

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“…As an example, urine is already being used to detect PCA3 in prostate and pancreatic cancer, and several state-of-the-art detection methods were developed for other body fluids [21]. Typically, in the development of an electrochemical sensor, a protein biomarker can be identified using antibody/antigen probes immobilized on the surface of the electrodes, aptamers specifically designed to bind the protein, and molecularly imprinted polymers (MIP) directly synthesized on the electrodes' surface, producing artificial antibodies [22][23][24].…”

Section: Essential Biomarkers For Liquid Biopsy Detection and Monitoringmentioning

confidence: 99%

Electrochemical Sensors for Liquid Biopsy and Their Integration into Lab-on-Chip Platforms: Revolutionizing the Approach to Diseases

Umme,

Siciliano,

Primiceri

et al. 2023

Chemosensors

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The screening and early diagnosis of diseases are crucial for a patient’s treatment to be successful and to improve their survival rate, especially for cancer. The development of non-invasive analytical methods able to detect the biomarkers of pathologies is a critical point to define a successful treatment and a good outcome. This study extensively reviews the electrochemical methods used for the development of biosensors in a liquid biopsy, owing to their ability to provide a rapid response, precise detection, and low detection limits. We also discuss new developments in electrochemical biosensors, which can improve the specificity and sensitivity of standard analytical procedures. Electrochemical biosensors demonstrate remarkable sensitivity in detecting minute quantities of analytes, encompassing proteins, nucleic acids, and circulating tumor cells, even within challenging matrices such as urine, serum, blood, and various other body fluids. Among the various detection techniques used for the detection of cancer biomarkers, even in the picogram range, voltammetric sensors are deeply discussed in this review because of their advantages and technical characteristics. This widespread utilization stems from their ability to facilitate the quantitative detection of ions and molecules with exceptional precision. A comparison of each electrochemical technique is discussed to assist with the selection of appropriate analytical methods.

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Development of an MIP based electrochemical sensor for TGF-β1 detection and its application in liquid biopsy

Abstract: Oral cancer is one of the most common types of cancer in Europe Oral cancer is one of the most common types of cancer in Europe and its large diffusion...

Cited by 9 publications

References 40 publications

Beyond blood: Advancing the frontiers of liquid biopsy in oncology and personalized medicine

Beyond blood: Advancing the frontiers of liquid biopsy in oncology and personalized medicine

Application of Sensing Devices in the Detection of Oral, Pulmonary, and Gastrointestinal Diseases

Electrochemical Sensors for Liquid Biopsy and Their Integration into Lab-on-Chip Platforms: Revolutionizing the Approach to Diseases

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