The applicability of 454 pyrosequencing to characterize bacterial biofilm communities from two water meters of a drinking water distribution system was assessed. Differences in bacterial diversity and composition were observed. A better understanding of the bacterial ecology of drinking water biofilms will allow for effective management of water quality in distribution systems.Drinking water distribution systems (DWDS) are interesting model systems for studies of microbial diversity and ecosystem functions in engineered environments. DWDS biofilms in particular have received much focus due to the importance of potable water delivery to end-point consumers (8). Both cultivation-based and molecular approaches have been used to reveal bacterial communities from different locations or pipe materials within a DWDS and from DWDS that differ in source water and nutrients. Studies of bacterial communities throughout DWDS have indicated that populations can differ drastically from source water to tap water (7). Activities of microbial growth and the presence of potential opportunistic pathogens have been detected in tap water, faucets, and showerheads, etc., all end points of the DWDS (8,14). Many studies attribute the survival of opportunistic pathogens in DWDS to resistance mechanisms such as cell wall permeability and biofilm formation (9, 10). The type of bacterial communities present in a DWDS and the disinfection regime applied may also be factors influencing pathogen retention in the DWDS. Nitrifying microorganisms, for example, can contribute to the depletion of monochloramine and subsequently lead to increased overall microbial growth (7).Compared to the analysis of microbial communities in source water and end-point drinking water, investigations related to the delivery process are difficult due to limited access and the high cost involved in sampling within the DWDS. To overcome these challenges, several studies have used model DWDS to mimic full-scale DWDS so as to address treatment effects on water quality. The researchers in these studies reported that episodic chlorination may accelerate the development of microbial communities with increased resistance to disinfectants (4) and that bacterial diversity can also affect disinfection efficacy and pathogen survival (1). In addition, the occurrence of bacterial community succession was observed in a model DWDS (17), which indicated the importance of longterm monitoring of bacterial biofilm development in the DWDS. These findings also demonstrated the need for the direct study of microbial communities within the DWDS, which would complement studies of the source water and end points, give a more complete view of the microbial ecology of the DWDS, and lend insights for subsequent monitoring of the DWDS.Biofilms obtained from water meters can be a good alternative to those obtained from distribution water pipes to study biofilms in DWDS because the water meters can be obtained relatively easily from private households. Moreover, the biofilms in the water meters no...