Biofilm Research Uncovers a Novel Nonenzymatic Signal Peptidase Function in Bacillus

Dijl, Jan Maarten van

doi:10.1128/jb.00270-12

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…Thus, it seems that SipW is a bifunctional SPI. Judged by the conservation of the C terminus, it seems that SipW proteins of other bacilli may have similar functions (166), but this remains to be demonstrated.…”

Section: Signal Peptidase Imentioning

confidence: 99%

Membrane Proteases in the Bacterial Protein Secretion and Quality Control Pathway

Dalbey

Wang

Dijl

2012

Microbiol Mol Biol Rev

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128

129

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SUMMARY Proteolytic cleavage of proteins that are permanently or transiently associated with the cytoplasmic membrane is crucially important for a wide range of essential processes in bacteria. This applies in particular to the secretion of proteins and to membrane protein quality control. Major progress has been made in elucidating the structure-function relationships of many of the responsible membrane proteases, including signal peptidases, signal peptide hydrolases, FtsH, the rhomboid protease GlpG, and the site 1 protease DegS. These enzymes employ very different mechanisms to cleave substrates at the cytoplasmic and extracytoplasmic membrane surfaces or within the plane of the membrane. This review highlights the different ways that bacterial membrane proteases degrade their substrates, with special emphasis on catalytic mechanisms and substrate delivery to the respective active sites.

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Section: Signal Peptidase Imentioning

confidence: 99%

Membrane Proteases in the Bacterial Protein Secretion and Quality Control Pathway

Dalbey

Wang

Dijl

2012

Microbiol Mol Biol Rev

Self Cite

128

129

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“…All these enzymes contribute to the acquisition of phosphate. A similar functional redundancy was previously shown for other proteins that fulfill critical physiological roles in B. subtilis as exemplified by eight exported proteases that cooperate in nitrogen acquisition (12) or essential protein secretion machinery components such as the five type I signal peptidases that catalyze the maturation of secretory precursor proteins (66). In this light, we believe that the demonstration of the metallophosphoesterase activity of YkuE is of physiological relevance.…”

Section: Discussionmentioning

confidence: 70%

Specific Targeting of the Metallophosphoesterase YkuE to the Bacillus Cell Wall Requires the Twin-arginine Translocation System

Monteferrante

Miethke

Ploeg

et al. 2012

Journal of Biological Chemistry

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EngineeringBacillusfor efficient production of heterologous protein: current progress, challenge and prospect

et al. 2019

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Summary Bacillus species are widely recognized as good industrial platform strains, which can produce a variety of valuable products including enzymes and functional proteins. Bacillus expression systems gain various competitive edges over other expression systems, with respect to nontoxicity, convenience for gene modification and high yield of target proteins. Recently, a number of Bacillus expression systems have been developed, and various strategies were conducted to improve the yields of target proteins. In this review, we focused on the strategies of host strain optimization for heterologous protein production, including secretion pathway optimization, RNA and protein stability enhancement, cell growth facilitation, genome streamlining and transcriptional regulator engineering. In addition, Bacillus spore surface display and food‐grade expression systems were developed to expand the application of Bacillus expression system in recent years. Finally, the challenges and prospects of Bacillus expression system were discussed regarding the recent progresses, challenges and trends in this field.

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Biofilm Research Uncovers a Novel Nonenzymatic Signal Peptidase Function in Bacillus

Cited by 3 publications

References 24 publications

Membrane Proteases in the Bacterial Protein Secretion and Quality Control Pathway

Membrane Proteases in the Bacterial Protein Secretion and Quality Control Pathway

Specific Targeting of the Metallophosphoesterase YkuE to the Bacillus Cell Wall Requires the Twin-arginine Translocation System

EngineeringBacillusfor efficient production of heterologous protein: current progress, challenge and prospect

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