Biofilm production of coagulase-negative staphylococci isolated from rescued wild animals in the Republic of Korea

Abstract

Biofilm production is a well-known causative factor of catheter- and medical device-related sepsis. Its high prevalence in coagulase-negative staphylococci (CoNS) has recently been reported. Information on biofilm production in CoNS isolated from wild animals is lacking. Herein, we studied the biofilm formation capabilities of CoNS isolated from rescued wild animals in the Republic of Korea. Swab samples were collected from the conjunctiva, nasal cavity, perianal area, and rectum for mammals while the sampling was done from the conjunctiva, oral mucosa, pericloacal area, and cloaca for birds. Isolation of CoNS was based on morphological and biochemical analyses along with molecular typing. Biofilm production was analyzed using 96-well plate based quantitative adherence assays. The studies demonstrated that CoNS of mammalian origin have higher biofilm-producing ability (70.4%) than the isolates from birds (62.5%). In particular, all methicillin-resistant (MR) CoNS isolated from mammals were capable of biofilm formation while only 63.3% of MR CoNS isolated from birds could produce biofilms. The MR CoNS isolated from mammals also had a significantly higher ability to form biofilms (100%) than methicillin susceptible CoNS (60.0%) than those isolates from birds. The findings show that wild animals may act as reservoirs as well as possible transmitters of biofilm-mediated antibiotic resistant genes.

Minimum inhibitory concentration of essential oils against Staphylococcus aureus isolated from dogs with external otitis

“…Moreover, S. aureus has several features related to antimicrobial resistance, including biofilm formation, which impairs the treatment of infected hosts (Swamy et al, 2016). Although biofilm formation was not evaluated in this study, this virulence factor is already known among S. aureus strains isolated from canine otitis, increasing the antimicrobial resistance and pathogenicity (Moreira et al, 2012;Lee et al, 2019).…”

Animal contact and paediatric acute febrile illness in Greater Accra Region, Ghana

“… 29 The infections detected in the present study, except HIV, could plausibly have been transmitted by animal contact, among diverse potential origins. 7 , 9 , 30 , 31 The HIV case was a ten-year-old boy, and we retained this case in the analysis because it was plausible that the observed symptoms were due to some other undetected infection, not HIV. This suggests that more research is needed to evaluate animal contact as a risk for bacterial zoonoses, which may serve as an etiological driver of acute febrile illness in sub-Saharan Africa.…”