Biofilm Microenvironments: Modeling Approach

Ghosh, Saheli; Qureshi, Asifa; Purohit, Hemant J.

doi:10.1007/978-981-10-6863-8_15

Cited by 9 publications

(6 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A biofilm is defined as a functional, phylogenetically diverse community of bacteria, fungi, and algae that adheres permanently to a surface [27]. Biofilm production ensures increased cell viability and nutrient availability.…”

Section: Discussionmentioning

confidence: 99%

Dishwashers as an Extreme Environment of Potentially Pathogenic Yeast Species

et al. 2021

View full text Add to dashboard Cite

The study aimed to compare the yeast species diversity in the specific environment of dishwashers, taking into account the potential risk for users. Yeasts were isolated from ten dishwashers and from tap water supplied to the appliances. Samples were collected for mycological analyses at the beginning of each month, from February to May 2016. Four dishwasher sites (rubber seals, detergent dispensers, sprinklers, and water drains) were analyzed. The microfungi were identified by the standard procedures applied in mycological diagnostics. To confirm species identification, molecular analysis was performed based on the sequences of the D1/D2 region. The presence of microfungi was detected in 70% of the investigated appliances. Rubber seals, detergent dispensers, and water drains were the most frequently colonized elements. Thirty-five yeast strains were isolated in this study, of which twenty-seven were obtained from dishwashers and eight from tap water. The strains belonged to six genera and six species (Candida parapsilosis, Clavispora lusitaniae, Dipodascus capitatus, Exophiala dermatitidis, Meyerozyma guilliermondii, and Rhodotorula mucilaginosa). Most of the strains came from rubber seals. In this way, it was demonstrated that the dishwashers’ condition is sufficient as an ecological niche for microfungi.

show abstract

Section: Discussionmentioning

confidence: 99%

Dishwashers as an Extreme Environment of Potentially Pathogenic Yeast Species

et al. 2021

View full text Add to dashboard Cite

show abstract

“…It facilitates colonization for other organisms. (Flemming, 1998;Ghosh et al, 2016;Ghosh et al, 2017b;Michels et al, 2018). Furthermore, biofilm interacts with the synthetic polymer in several ways.…”

Section: Biofilm-plastic Interactionsmentioning

confidence: 99%

“…Microorganisms adhered on the plastic surface forms a biofilm which degrades both natural and synthetic polymers (Gu, 2003). Biofilms are functionally, and phylogenetically diverse communities of bacteria, fungi, and algae, conjointly termed as a microbial conglomeration, attached to a surface (Ghosh et al, 2017b). They are mostly embedded in extracellular polymeric substance (EPS) (Ghosh et al, 2016;Ghosh et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Microbial degradation of plastics: Biofilms and degradation pathways

Ghosh¹,

Qureshi²,

Purohit³

2019

Contaminants in Agriculture and Environment: Health Risks and Remediation

View full text Add to dashboard Cite

Plastics are recalcitrant polymers released in the environment through unpredicted use leading to accumulation and increased water and soil pollution. Transportation of these recalcitrant polymers in agricultural soil, sediment, and water has been causing concerns for environmentalists. Biofilm community adhered on plastic polymers have a significant contribution in their degradation as they warrant bioavailability of substrates, sharing of metabolites and increased cell viability thereby accelerating biodegradation. Metabolic enzymes of the microbes can be exploited as a potent tool for polymer degradation. However very little or Chapter contents Introduction …………………………………………………………………………………………………….. Plastic degradation pathways in bacteria …………………………………………………………………… Natural metabolic pathways ……………………………………………………………………………… Engineered pathways ……………………………………………………………………………………… Biofilm forming microbes involved in degradation ……………………………………………………….. Bacteria ………………………………………………………………………………………………………. Fungus ………………………………………………………………………………………………………. Influence of biofilm on plastic degradation ………………………………………………………………... Biofilm-plastic interactions ………………………………………………………………………………….. Conclusion ……………………………………………………………………………………………………... Acknowledgments ……………………………………………………………………………………………. References ……………………………………………………………………………………………………… 185 no reports are available about the influence of biofilm and plastic degradation and vice versa. The present chapter reports the impact of biofilm microbes in the degradation of commonly used plastics. Furthermore, potent microorganisms and their interactions with the plastic surface has been deciphered, which would serve as a better understanding of the utilization of biofilm-based methods in the development of plastic waste management.

show abstract

“…The spatial structure in a bio lm matrix grants heterogeneous microenvironments, sharing of intermediates, horizontal gene transfers, maintains optimal physical conditions due to mass transfer balance (Palmer et al 2007;Flemming et al 2016;Abe et al 2020). This ensures deterrence against stress to the underlying members in the bio lm, leading to the evolution of altered physiology in a population of the same strain as well as in a community (Ghosh et al 2017b; Tsang and Simpson 2020). The cells are generally characterized by one or more phenotype(s), each with distinct degradative functionality (Ghosh et al, 2017a).…”

Section: Introductionmentioning

confidence: 99%

Managing gene expression in Pseudomonas simiae EGD-AQ6 for chloroaromatic compound degradation

2022

Self Cite

View full text Add to dashboard Cite

Pseudomonas simiae EGD-AQ6 showed utilization of chloroaromatic compound, 2-4dichlorophenoxyacetic acid (2,4-D) e ciently in its bio lm phenotype. The differential rates of accumulation of intermediate metabolite 4-chlorocatechol (4-CCA) were signi cant in both planktonic and bio lm phenotypes; also increased number of bio lm cells were observed during 2,4-D utilization.Interestingly, response surface analysis demonstrated the combined positive effects of 2,4-D degradation and 4-CCA accumulations. Also, gene expression pro les showed signi cant up-regulation of degradative and bio lm genes (particularly pellicle forming genes) in the bio lm phenotypes than their planktonic counterparts, thereby con rming occurrence of phenotype variations of Pseudomonas simiae EGD-AQ6 during chloroaromatic degradation. Furthermore, the sequence similarity of the 2-4-D catabolic genes and bio lm forming proteins (pel ABCDEFG and pga ABCD) which are responsible for building carbohydrate rich extracellular matrix, were signi cant with the respective organisms as revealed through genome analysis. This is the rst report, which endorses this Pseudomonas simiae species to be unique in chloroaromatic degradation through phenotype variation, thereby proving as a potential candidate in the improvement of bioremediation technologies.

show abstract

Biofilm Microenvironments: Modeling Approach

Cited by 9 publications

References 75 publications

Dishwashers as an Extreme Environment of Potentially Pathogenic Yeast Species

Dishwashers as an Extreme Environment of Potentially Pathogenic Yeast Species

Microbial degradation of plastics: Biofilms and degradation pathways

Managing gene expression in Pseudomonas simiae EGD-AQ6 for chloroaromatic compound degradation

Contact Info

Product

Resources

About