Biofilm-Mediated Heavy Metals Bioremediation in PGPR Pseudomonas

Meliani, Amina; Bensoltane, Ahmed

doi:10.4172/2155-6199.1000370

Cited by 45 publications

(16 citation statements)

References 41 publications

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“…3). There have been instances of bio lms being used to remove heavy metals (Meliani and Bensoltane, 2016;Ogbuagu et al, 2017). Bio lm communities of Gram positive and Gram negative bacteria, including Streptococcus aureus, B. subtilis, B. licheniformis, Pseudomonas aeruginosa and Serratia marscecens have been reported to have Cd 2+ and Zn 2+ bioremediated (Khan et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Fungal-bacterial biofilm mediated heavy metal rhizo-remediation

Henagamage

Peries

Seneviratne

2021

Preprint

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Heavy metal pollution due to excessive use of chemical fertilizers (CF) causes a major damage to the environment. Microbial consortia, closely associated with the rhizosphere are able to remediate heavy metal-contaminated soil by reducing plant toxicity. Thus, this study was undertaken to examine the remedial effects of microbial biofilms against contaminated heavy metals. Fungi and bacteria isolated from soil were screened for their tolerance against Cd2+, Pb2+ and Zn2+. Fungal-bacterial biofilms (FBBs) were developed with the highest tolerant isolates and were further screened for their bioremediation capabilities against heavy metals. The best biofilm was evaluated for its rhizoremediation capability with different CF combinations using a pot experiment conducted under greenhouse conditions with potato. Three bacterial and two fungal isolates were selected to develop FBBs upon the tolerance index (TI) percentage. Significantly (P < 0.05) the highest metal removal percentage was observed in Trichoderma harzianum and Bacillus subtilis biofilm under in situ condition. The biofilm with 50% of recommended CF (50CB) significantly (P < 0.05) reduced the soil available Pb2+ by 77%, Cd2+ by 78% and Zn2+ by 62% compared to 100% recommended CF (100C). In comparison to initial soil, it was 73%, 76% and 57% lower of Pb2+, Cd2+ and Zn2+, respectively. In addition, 50CB treatment significantly (P < 0.05) reduced the metal penetration into the tuber tissues in comparison with 100C. Thus, it is concluded that T. harzianum–B. subtilis biofilm is an ideal combination to remediate soil contaminated with Cd2+, Pb2+ and Zn2+.

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Section: Discussionmentioning

confidence: 99%

Fungal-bacterial biofilm mediated heavy metal rhizo-remediation

Henagamage

Peries

Seneviratne

2021

Preprint

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“…At the same time, microorganisms show great promise as biosensors to quantify the bioavailable fraction of heavy metals such as copper [ 13 ], an essential trace element that is highly toxic when overly abundant [ 14 ]. In addition, microorganisms can be used to combat environmental contamination with heavy metals in a process called bioremediation [ 3 , 8 , 15 , 16 ]. Bioremediation has been the focus of extensive research in recent years as a clean and efficient alternative to conventional strategies (reviewed in Tabak et al, 2005 [ 17 ] and Akcil et al, 2015 [ 18 ]).…”

Section: Introductionmentioning

confidence: 99%

The Transcriptomic Landscape of Cupriavidus metallidurans CH34 Acutely Exposed to Copper

Maertens

Leys

Matroule

2020

Genes

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Bacteria are increasingly used for biotechnological applications such as bioremediation, biorecovery, bioproduction, and biosensing. The development of strains suited for such applications requires a thorough understanding of their behavior, with a key role for their transcriptomic landscape. We present a thorough analysis of the transcriptome of Cupriavidus metallidurans CH34 cells acutely exposed to copper by tagRNA-sequencing. C. metallidurans CH34 is a model organism for metal resistance, and its potential as a biosensor and candidate for metal bioremediation has been demonstrated in multiple studies. Several metabolic pathways were impacted by Cu exposure, and a broad spectrum of metal resistance mechanisms, not limited to copper-specific clusters, was overexpressed. In addition, several gene clusters involved in the oxidative stress response and the cysteine-sulfur metabolism were induced. In total, 7500 transcription start sites (TSSs) were annotated and classified with respect to their location relative to coding sequences (CDSs). Predicted TSSs were used to re-annotate 182 CDSs. The TSSs of 2422 CDSs were detected, and consensus promotor logos were derived. Interestingly, many leaderless messenger RNAs (mRNAs) were found. In addition, many mRNAs were transcribed from multiple alternative TSSs. We observed pervasive intragenic TSSs both in sense and antisense to CDSs. Antisense transcripts were enriched near the 5′ end of mRNAs, indicating a functional role in post-transcriptional regulation. In total, 578 TSSs were detected in intergenic regions, of which 35 were identified as putative small regulatory RNAs. Finally, we provide a detailed analysis of the main copper resistance clusters in CH34, which include many intragenic and antisense transcripts. These results clearly highlight the ubiquity of noncoding transcripts in the CH34 transcriptome, many of which are putatively involved in the regulation of metal resistance.

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“…These metals have been shown to be more toxic, reducing biofilm growth [60,61]. Biofilms (including those formed by Pseudomonas species) can develop resistance mechanisms for heavy metals over the long term [62,63]. In this study, the Pseudomonas were not exposed to the metals for long enough to develop resistance.…”

Section: Biofilm Formation Analysismentioning

confidence: 94%

Development of Ceramic Water Filter Clay Selection Criteria

Shepard

Zhang

Anaya

et al. 2020

Water

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Ceramic water filters (CWFs) are point-of-use drinking water treatment systems that are manufactured and used in under-served communities around the world. The clayey material (CM) used to manufacture CWFs is a locally sourced mixture of clay, sand, slit and amorphous material (usually dug near the CWF factory). CM varies in composition and purity depending on the geographical location and geological setting. In this study, a set of 13 CM samples collected from around the world were analyzed using grain size analysis, as well as liquid and plastic limit tests. Mineralogical composition was determined using X-ray diffraction. A selection of three CM samples (Guatemala, Canada, and Guinea Bissau) with a range of compositions were used to study biofilm growth on CM before and after firing. Biofilm coverage was studied on CM (before firing) and CWF material (after firing) using Pseudomonas fluorescens Migula. The average biofilm coverages for Guatemala, Canada, and Guinea Bissau CM were 20.03 ± 2.80%, 19.28 ± 0.91%, and 9.88 ± 4.02%, respectively. The average biofilm formation coverages for Guatemala, Canada, and Guinea Bissau CWF were 13.08 ± 1.74%, 10.36 ± 3.41%, and 8.66 ± 0.13%, respectively. The results presented here suggest that CM can be manipulated to manufacture better performing CWFs by engineering the soil characteristics, such as grain size, liquid and plastic limits, and mineralogy. This could improve the durability and biofilm resistance of CWFs.

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Biofilm-Mediated Heavy Metals Bioremediation in PGPR Pseudomonas

Cited by 45 publications

References 41 publications

Fungal-bacterial biofilm mediated heavy metal rhizo-remediation

Fungal-bacterial biofilm mediated heavy metal rhizo-remediation

The Transcriptomic Landscape of Cupriavidus metallidurans CH34 Acutely Exposed to Copper

Development of Ceramic Water Filter Clay Selection Criteria

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