Biofilm-forming ability profiling of Staphylococcus aureus and Staphylococcus epidermidis mastitis isolates

Oliveira, Manuela; Bexiga, Ricardo; Nunes, Sónia; Carneiro, C.; Cavaco, Lina; Bernardo, Fernando; Vilela, C. L.

doi:10.1016/j.vetmic.2006.07.008

Cited by 117 publications

(104 citation statements)

References 23 publications

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“…No association was observed between phenotypic and molecular tests for bioϐilm production, corroborating previous studies (Baselga et al 1993, Arciola et al 2001, Vasuvedan et al 2003, Oliveira et al 2006. Only seven isolates were positive for both methods simultaneously.…”

Section: Discussionsupporting

confidence: 88%

“…PCR detected more bioϐilm forming isolates than the Congo red agar test, supporting previous ϐindings (Oliveira et al 2006). However, the present study focused on the icaD gene alone, while other genetic loci (bap, agr and sar) might be associated with bioϐilm formation as well (Oliveira et al 2006). The main occurrence of bioϐilm in goat isolates is associated with antimicrobial resistance and multidrug resistance, as reported in previous studies (Baselga et al 1993, Oliveira et al, 2006.…”

Section: Discussionsupporting

confidence: 87%

“…The Congo Red Agar test is a very simple and inexpensive technique, but correct interpretation of the results depends on many factors, such as colony color characteristics (Baselga et al 1993). PCR detected more bioϐilm forming isolates than the Congo red agar test, supporting previous ϐindings (Oliveira et al 2006). However, the present study focused on the icaD gene alone, while other genetic loci (bap, agr and sar) might be associated with bioϐilm formation as well (Oliveira et al 2006).…”

Section: Discussionsupporting

confidence: 84%

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Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil

et al. 2012

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Pesq. Vet. Bras. 32(8) The study aimed to determine the antimicrobial resistance patterns and to identify molecular resistance markers in Staphylococcus spp. (n=210) isolated from small ruminant mastitis in Brazil. The antimicrobial resistance patterns were evaluated by the disk diffusion test and by detection of the presence of mecA, blaZ, ermA, ermB, ermC and msrA genes by PCR. The efϐlux pump test was performed using ethidium bromide and bioϐilm production was determined by Congo red agar test along with PCR for detection of the icaD gene. The isolates were most resistant to amoxicillin (50.0%), streptomycin (42.8%), tetracycline (40.4%), lincomycin (39.0%) and erythromycin (33.8%). Pan-susceptibility to all tested drugs was observed in 71 (33.8%) isolates and 41 Staphylococcus isolates were positive for the efϐlux pump. Although phenotypic resistance to oxacillin was observed in 12.8% of the isolates, none harbored the mecA gene. However, 45.7% of the isolates harbored blaZ indicating that beta-lactamase production was the main mechanism associated with staphylococci resistance to beta-lactams in the present study. The other determinants of resistance to antimicrobial agents ermA, ermB, ermC, and msrA were observed in 1.4%, 10.4%, 16.2%, and 0.9% of the isolates, respectively. In addition, the icaD gen was detected in 32.9% of the isolates. Seventy three isolates (54 from goats and 19 from sheep) were negative for all resistance genes tested and 69 isolates presented two or more resistance genes. Association among blaZ, ermA, ermB, ermC and efϐlux pump were observed in 17 isolates, 14 of which originated from goats and three from sheep. The data obtained in this study show the resistance of the isolates to beta-lactamics, which may be associated with the use of antimicrobial drugs without veterinary control.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 84%

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Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil

et al. 2012

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“…Oliveira et al (2006) detected biofilm formation on CRA in 37.5% of S. aureus strains isolated from cases of subclinical mastitis, a rate similar to that observed in the present study. The authors also found similar biofilm formation by S. epidermidis and S. aureus, in contrast to the present study in which a lower percentage (13.33%) of CNS produced biofilms.…”

Section: Biofilm Production and Antimicrobial Susceptibilitysupporting

confidence: 91%

Intramammary treatment with gentamicin in lactating cows with clinical and subclinical mastitis

et al. 2016

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The study evaluated the microbiological profile of milk samples collected before and after mastitis treatment with gentamicin and investigated biofilms production and antimicrobial susceptibility of Staphylococcus spp. isolated. The presence of gentamicin residues in milk after the recommended withdrawal period was also evaluated. Antimicrobial residues were analyzed by Delvotest® SP NT over a period of 12 days beginning after 24 hours the last gentamicin application. Some of Staphylococcus spp. isolates were biofilm producers (19.05%). Staphylococcus spp. showed high levels of resistance to neomycin (16.95%), penicillin G (10.17%), and ampicillin (10.17%). Multidrug resistance to all antibiotics tested was observed in 1.69% of the Staphylococcus spp. isolates. Among 1440 mammary quarter milk samples 24.95% presented gentamicin residues after the withdrawal period. Gentamicin residues were also detected in 3.8% of samples from calibrated glass recorder jar (n=383) 4.1 days after treatment. The indiscriminate use of antibiotics may lead to the emergence of multidrug-resistant strains as well as increasing the risk of presence of residues of these drugs in milk. These problems affect the milk quality and may become a public health problem.

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“…A formação do biofilme inicia com a adesão da célula bacteriana na superfície, seguida da sua maturação, ruptura e dispersão (Vermelho et al 2007). Sabe-se que S. aureus e S. epidermidis, oriundos de casos de mastite, possuem alta capacidade de adesão em superfícies abióticas, formando, posteriormente, biofilmes altamente organizados (Oliveira et al 2006). Tendo em vista a importância da adesão no processo de colonização e invasão de micro-organismos patogênicos, o uso in vitro de antimicrobianos na sua prevenção está muito bem documentado (Pagano et al 2004).…”

Section: Introductionunclassified

Ação dos desinfetantes sobre a adesão e biofilme consolidado de Staphylococcus spp.

et al. 2015

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RESUMO.-Staphylococcus spp. são reconhecidos como importantes causadores de mastites em rebanhos leiteiros. Esses micro-organismos têm a capacidade de produzir uma estrutura denominada biofilme, que é responsável pela sobrevivência e muitas vezes pela resistência a ação de produtos desinfetantes e as demais condições adversas. Neste trabalho avaliou-se a ação de dois produtos pós-dipping a base de iodo (0,7%) e clorexidine (2,0%) sobre a adesão de Staphylococcus coagulase positiva (SCP) e Staphylococcus coagulase negativa (SCN) isolados de casos de mastite subclínica, e também sobre biofilmes pré formados a partir destes isolados. Os produtos testados apresentaram uma alta redução na taxa de adesão de todos os isolados. No entanto, a ação sobre os biofilmes consolidados só foi estatisticamente significativa sobre os SCN. Assim, ressalta-se a importância dos programas sanitários a fim de prevenir a formação de biofilmes e diminuir as fontes de contaminação da glândula mamaria em sistemas de produção leiteira.TERMOS DE INDEXACAO: Staphylococcus spp., pós-dipping, desinfetantes, mastite, prevenção. INTRODUÇÃOA mastite infecciosa bovina é uma enfermidade associada à redução da produção e alterações na composição do leite, bem como ao descarte de animais (Barlow 2011). Os micro-organismos de origem contagiosa são os mais prevalentes em casos de mastite bovina e, entre esses, o genero Staphylococcus destaca-se por estar frequentemente associado a casos clínicos e subclínicos da enfermidade (Sá et al. 2004). São muitos os fatores de risco envolvidos na ocorrência de mastite em rebanhos leiteiros. Destacamos neste estudo a exposição da superfície dos tetos aos micro--organismos patogênicos contagiosos, os quais são transmitidos de animais infectados para não infectados durante o processo de ordenha (Amaral et al. 2004 Staphylococcus spp. are recognized as important causes of mastitis in dairy herds. These microorganisms have the ability to produce a structure called biofilm, which is responsible for the survival and often for resistance to the action of disinfectants and other harsh conditions. In this paper we evaluate the action of two teat dip products based on iodine (0,7%) and chlorhexidine (2,0%) on the adhesion of coagulase-positive staphylococci (CPS) and coagulase negative staphylococci (CNS) from subclinical mastitis cases, and also on its consolidate biofilm. The products tested showed a high reduction on the adhesion of all isolates. However, action on the consolidate biofilm was significant for CNS only, and unsatisfactory for CPS. Thus, it emphasizes the importance of health programs to prevent biofilm formation and reduce the sources of contamination of the mammary gland in dairy production systems.

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Biofilm-forming ability profiling of Staphylococcus aureus and Staphylococcus epidermidis mastitis isolates

Cited by 117 publications

References 23 publications

Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil

Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil

Intramammary treatment with gentamicin in lactating cows with clinical and subclinical mastitis

Ação dos desinfetantes sobre a adesão e biofilme consolidado de Staphylococcus spp.

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