Biofilm-forming ability of poultry Campylobacter jejuni strains in the presence and absence of Pseudomonas aeruginosa

Abstract

The aims of this study were to evaluate the ability of Campylobacter jejuni isolated from a poultry slaughterhouse to form biofilm in the presence and absence of Pseudomonas aeruginosa, and the effect of surface (stainless steel, polystyrene), temperature (7, 25, and 42 °C), and oxygen concentration (microaerophilic and aerobic conditions) on the formation of biofilm. The genes ahpC, cadF, clpP, dnaJ, docA, flaA, flaB, katA, kpsM, luxS, racR, and sodB, related to biofilm formation by C. jejuni, were also investigated. All isolates formed biofilm on stainless steel and on polystyrene, in both aerobic and microaerophilic atmospheres, including temperatures not optimal for C. jejuni growth (7 and 25 °C), and biofilm also was formed in the presence of P. aeruginosa. In dual-species biofilm on stainless steel, biofilm formation was 2–6 log CFU·cm−2 higher at 7 °C for all isolates, in comparison with monospecies biofilm. Ten genes (ahpC, cadF, clpP, dnaJ, docA, flaA, flaB, luxS, racR, and sodB) were detected in all isolates, but katA and kpsM were found in four and six isolates, respectively. The results obtained are of concern because the poultry C. jejuni isolates form biofilm in different conditions, which is enhanced in the presence of other biofilm formers, such as P. aeruginosa.

Effect of Biofilm on Production of Poultry

“…Campylobacter is also a pathogenic bacterium in poultry flock and several genes responsible for production of biofilm in surfaces of stainless steel and polystyrine articles at different temperatures and oxygen concentration. The genes responsible for production of biofilm are bhpC, cadF, clpP, dnaJ, docA., flaA, flaB, katA, kspM, luxS, racR and sodB [49].…”

Campylobacter Biofilms: Potential of Natural Compounds to Disrupt Campylobacter jejuni Transmission

“…There are a number of genes known to be involved in the biofilm formation process and include those responsible for motility and chemotaxis [58][59][60], lipooligosaccharide biosynthesis [58,59,61,62], N-linked protein glycosylation, capsular polysaccharides (CPS) [58,62,63], and stress response proteins. Quorum sensing (QS), which allows the bacteria to regulate population cell density in biofilms was also found to play a role in Campylobacter biofilm formation and to contribute to host colonisation [40,60,64,65]. However, an important messenger, the intercellular bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP), which plays an essential role in the transition between sessile and motile lifestyles in many other organisms [66], or its homologue, is yet to be found in the C. jejuni genome.…”

Antibiofilm Effects of Heated Scallop Shell Powder on Campylobacter jejuni Biofilms

“…Campylobacteriosis has been the most common cause of bacterial food poisoning in Japan for more than a decade, and C. jejuni is the predominant species responsible for campylobacteriosis (Japan's Ministry of Health, Labor, and Welfare statistical survey for 2020). C. jejuni can form biofilms [2] that can protect microorganisms from environmental stress and antimicrobial agents, and some studies have indicated that bacteria in biofilms are as much as 1000 times more resistant to antibiotics than planktonic cells [3][4][5].…”