Biofilm forming abilities of Salmonellaare correlated with persistence in fish meal- and feed factories

Vestby, Lene K.; Møretrø, Trond; Langsrud, Solveig; Heir, Even; Nesse, Live L.

doi:10.1186/1746-6148-5-20

Cited by 226 publications

(212 citation statements)

References 28 publications

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“…These biofilms allow the persistence of Salmonella spp. in feed and food factory environments for months, and even years (Vestby et al, 2009;Schonewille et al, 2012;Prunić et al, 2016).…”

Section: B I O F I L M F O R M I N G C a Pa C I T Y O F Salmonella Spmentioning

confidence: 99%

Salmonella spp. in poultry: a constant challenge and new insights

Velhner

Milanov

Kozoderović

2018

J Hellenic Vet Med Soc

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ABSTRACT. The knowledge about virulence mechanisms, resistance to antimicrobial agents and the biofilm formation ability of Salmonella spp. in poultry industry has been expanded over the years. However, in spite of the research efforts and significant investments to improve management systems in poultry industry, it has become evident that none of the methods applied in all stages of food production chain are 100% effective in eliminating Salmonella spp. Different serovars are manifesting different mechanisms of invasiveness which depend on their ability to invade lower zones of the lamina propria, their ability to gain accesses to parenchymatous organs and survive in macrophages. The ubiquitous nature of Salmonella spp. due to their adaptation to animal and plant hosts, as well as their survival in hostile environments and their enhanced capacity to produce biofilms, contribute to a long lasting contamination of the environment, feed and animals. The emergency and spread of antimicrobial resistances in Salmonella spp. raise additional concerns.

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“…These biofilms allow the persistence of Salmonella spp. in feed and food factory environments for months, and even years (Vestby et al, 2009;Schonewille et al, 2012;Prunić et al, 2016).…”

Section: B I O F I L M F O R M I N G C a Pa C I T Y O F Salmonella Spmentioning

confidence: 99%

Salmonella spp. in poultry: a constant challenge and new insights

Velhner

Milanov

Kozoderović

2018

J Hellenic Vet Med Soc

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“…This enables the growth of anaerobic and microaerophilic bacteria in biofilm and thus provides advantageous conditions for the development of biofilms. It was determined that the occurrence of rigid pellicle structure requires a regulation of the functionality of the genes that are effective on the biofilm formation (Stepanović et al, 2004;Latasa et al, 2005;Vestby et al, 2009). In the trials investigating the effect of created mutations on the pellicle structure, no change in the properties of the pellicle structure was determined in rmbA, fidL, misL, yliH, fliC, yliH, and cheM gene mutants when compared to the wild type.…”

Section: Discussionmentioning

confidence: 99%

“…Biofilm formation assay was performed in 96-well polystyrene microtiter plates according to the method described by Woodward et al (2000), with some slight modifications as suggested by Stepanović et al (2000) and Vestby et al (2009). Briefly, overnight cultures were diluted in LB without NaCl (LB wo /NaCl) to OD 595 = 0.2, and 30 µL of this suspension was transferred to each well of the polystyrene plates containing 100 µL of LB wo /NaCl (3 parallels of each strain).…”

Section: Microtiter Plate Assaymentioning

confidence: 99%

The effect of marT gene on biofilm production of Salmonella Typhimurium

İlçe

Akçelik²

2015

Turk J Biol

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This study investigates the effect of MarT regulator protein on the production of Salmonella Typhimurium biofilm under in vitro culture conditions. The biofilm formation showed a statistically significant decrease compared to the wild-type strain at the two consecutive concentrations of arabinose (0.02% and 0.01%) in which the production of MarT protein was promoted to the highest level. When comparing the biofilm formation capacity to the wild type, the mutants containing a mutation in the genes investigated in terms of marT regulation showed statistically significant reductions, which were identified as fliA (59%), fliC (40%), fimA (46%), fidL (25%), and misL (25%) mutants. The biofilm structures of all strains used in this study were detected as the rdar morphotype in agar plates. Among the genes examined, fimA, slsA, fliA, dps, cheA, and wzzB genes were found to have a direct effect on the pellicle formation of S. Typhimurium. Finally, the biofilm formation features of the mutant strain, in which marT expression is induced, on glass, stainless steel, and polystyrene were also examined, and it was determined that the ability to produce biofilm decreased on all surfaces.

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“…however, this serovar was frequently detected in animal feed, which is a gate for microbial entrance into the food chain. Non-typhi serotypes of Salmonella enterica from feeds induce colonization or infection in foodproducing animals, hence posing an indirect risk for human health (CrumP et al, 2002;ALVAREZ et al, 2003;PAPADoPouLou et al, 2009;VESTBy et al, 2009). apparently, serovars present in animal feed differ from those commonly identified in humans, which is most probably because different strains survive in different environments (ALVAREZ et al, 2003).…”

Section: Congo Red Agar Test All S Tennessee Isolates (mentioning

confidence: 99%

“…The biofilm organization pattern of salmonellas is most likely responsible for their increased resistance to a range of stress factors, such as long-term desiccation, low nutrients, and disinfectants from feed and the food factory environment AVILES et al, 2013). Such multicellular behavior (biofilm) enables salmonellas to persist in the feed factory environment for months, even years (NeSSe et al, 2003;VESTBy et al, 2009;møretrø et al, 2009). Salmonella biofilms from equipment and food contact surfaces are a well-established source of long-lasting contamination of final products, which has been repeatedly confirmed by molecular methods (NeSSe et al, 2003;PAPADoPouLou et al, 2009). In Great Britain, S. Tennessee is one of the top five most isolated serovars from feed for cattle, pigs and poultry (PAPADoPouLou et al, 2009).…”

mentioning

confidence: 99%

Biofilm forming ability of Salmonella enterica serovar Tennessee isolates originating from feed

Milanov¹,

Prunić²,

Ljubojević³

2017

Vet. arhiv

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Salmonella enterica subspecies enterica serovar tennessee was the most frequent Salmonella serovar isolated from plant-based animal feed throughout a two-year period (2012)(2013) of research conducted in the region of the Autonomous Province of Vojvodina. In this study, the ability of biofilm formation was investigated on 20 isolates of S. Tennessee. The starting point for this research was the established biofilm-forming ability of serovar Tennessee strains, which significantly contributes to their wide distribution in the environment (plant materials), and hence in animal feed. In this study, biofilm formation was investigated on polystyrene (microplate biofilm assay) and stainless steel (scanning electron microscopy) surfaces by culturing in Luria Bertani broth (LB) and Triptone Soya Broth (TSB). The expression of major matrix components of Salmonella biofilm (curli fimbriae and cellulose) was examined by cultivation on Congo Red agar. All isolates of S. tennessee produced a biofilm on a polystyrene surface in a microtiter plate test, by cultivation in LB at an incubation temperature of 20 °C. Scanning electron microscopy revealed biofilm formation of S. tennessee on a stainless steel surface by cultivation in LB, but not in TSB. The expression of curli fimbriae and cellulose, as well as the formation of characteristic "rdar" colonies on Congo red agar has been observed in all isolates of S. tennessee. this serovar is not specifically adapted to humans and animals, so the available epidemiological data do not indicate its major implication in food-borne infection outbreaks. however, this serovar manifests a pronounced ability for adherence to plant surfaces, biofilm formation and increased resistance to long-term desiccation, which is the most likely explanation for its frequent identification in low water activity feed.

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Biofilm forming abilities of Salmonellaare correlated with persistence in fish meal- and feed factories

Cited by 226 publications

References 28 publications

Salmonella spp. in poultry: a constant challenge and new insights

Salmonella spp. in poultry: a constant challenge and new insights

The effect of marT gene on biofilm production of Salmonella Typhimurium

Biofilm forming ability of Salmonella enterica serovar Tennessee isolates originating from feed

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