Biofilm Formation by Gram-Negative Bacteria during Implant-Associated Infection

Babushkina, I. V.; Bondarenko, Alexander S.; Ulyanov, Vladimir Yu.; Mamonova, I. A.

doi:10.1007/s10517-020-04888-5

Cited by 11 publications

(13 citation statements)

References 4 publications

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“…Thus, the analyzed P. aeruginosa strains isolated from patients with infectious complications after large joint replacements were resistant to high concentrations of antibiotics if they form a biofilm, which confirms high protective functions of biofilm matrix [5,9]. It was established that the resistance of sessile P. aeruginosa strains was higher than that of their plankton form by 20-920 times for fluoroquinolones, 18-23 times for cephalosporins, and 71 times for amikacin.…”

Section: Resultsmentioning

confidence: 65%

“…Treatment of infectious inflammatory complications after total replacements of large joints remains a challenging issue due to the severity of state in these patients, complexity of bacteria diagnosis in this pathology, and empiric etiotropic treatment [4]. Implantassociated infections (IAI) caused by gram-negative bacteria including Pseudomonas aeruginosa that exhibits high resistance to antibiotics and liability to biofilm formation are still a problem in orthopedic surgery [5]. In a biofilm formed by gram-negative bacteria, virulence factors can be activated due to quorum sensing [7].…”

mentioning

confidence: 99%

“…In a biofilm formed by gram-negative bacteria, virulence factors can be activated due to quorum sensing [7]. P. aeruginosa can form biofilms within 24-48 h, which makes etiotropic treatment inefficient and often leads to the development of chronic IAI [5,7]. It was established that some antibiotics in concentrations used in clinical practice can stimulate biofilm growth [1,2].…”

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confidence: 99%

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Resistance to Antibiotics in Plankton and Biofilm Cultures of Pseudomonas aeruginosa Clinical Strains

et al. 2021

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Biofilms formed by Pseudomonas aeruginosa strains isolated from biomaterial of patients with implant-associated infection are characterized by much higher resistance to antibiotics of various classes than plankton cultures of these strains. The concentrations of antibiotics causing the death of 90% of P. aeruginosa biofilm (MIC 90 ) was 2-6 µg/ml for fluoroquinolones, 267-356 µg/ml for cephalosporins, and 92-215 µg/ml for amikacin, which significantly (p<0.05) differed from MIC 90 for plankton cultures that did not exceed 0.8 µg/ml for fluoroquinolones, 19 µg/ml for cephalosporins, and 3 µg/ml for amikacin. The degree of the microbial biofilm maturity also affected antibiotic resistance.

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Section: Resultsmentioning

confidence: 65%

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confidence: 99%

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confidence: 99%

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Resistance to Antibiotics in Plankton and Biofilm Cultures of Pseudomonas aeruginosa Clinical Strains

et al. 2021

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“…Much of our understanding of the microbial behaviour of BRII comes from in vitro studies, and a diversity of approaches have been used [33,[36][37][38][39][40][41][42][43][44]. Additionally, clinical, wild-type, bacterial isolates demonstrate phenotypical characteristics that differ significantly from typical laboratory reference strains of the same species; clinical samples tend to be more avid biofilm formers, and bacteria isolated from the disruption of biofilms through sonication form biofilms more readily than organisms obtained through aspiration of joint fluid [11,45]. Ultimately, any in vitro model of orthopaedic BRII is a compromise between creating a realistic model of the in vivo environment versus creating a versatile model that can be reproduced, so any in vitro result must be taken in this context [46].…”

Section: Bacteria In Orthopaedic Implant-related Infectionsmentioning

confidence: 99%

Recent Strategies to Combat Infections from Biofilm-Forming Bacteria on Orthopaedic Implants

Rodríguez-Merchán

Davidson

Liddle

2021

IJMS

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Biofilm-related implant infections (BRII) are a disastrous complication of both elective and trauma orthopaedic surgery and occur when an implant becomes colonised by bacteria. The definitive treatment to eradicate the infections once a biofilm has established is surgical excision of the implant and thorough local debridement, but this carries a significant socioeconomic cost, the outcomes for the patient are often poor, and there is a significant risk of recurrence. Due to the large volumes of surgical procedures performed annually involving medical device implantation, both in orthopaedic surgery and healthcare in general, and with the incidence of implant-related infection being as high as 5%, interventions to prevent and treat BRII are a major focus of research. As such, innovation is progressing at a very fast pace; the aim of this study is to review the latest interventions for the prevention and treatment of BRII, with a particular focus on implant-related approaches.

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“…5 to 34 percent of patients with periprosthetic infection receive false-negative results to their bacteriological tests. Nowadays the examination of the immune status in patients suspected of having periprosthetic infection is getting promising as it’s aimed at determining the most relevant indicators that may form the basis for the diagnostics of this pathology [2, 3].…”

Section: Introductionmentioning

confidence: 99%

Immunity status in patients with periprosthetic knee joint infection

Mamonova¹,

Babushkina²,

Bondarenko

et al. 2021

Preprint

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Mamonova I.A., Babushkina I.V., Bondarenko A.S., Ulyanov V.Yu. Immunity status in patients with periprosthetic knee joint infection. Implant-associated infection is one of the most severe complications after total replacement of larger joints. The diagnosis of periprosthetic infection is often impeded as 5-34% of bacteriological tests are false-negative. Now the investigations of the immune status of patients suspected of periprosthetic infection are considered perspective as these are aimed at finding the most significant indicators for underlying the diagnosis of this pathology. In this research, we assessed immunology indicators in peripheral blood of patients with periprosthetic infection in their knee joints. We determined immunology characteristics associated with the clinical aspects of the progressing pathology. Our findings can be used as the basis for further search of pathophysiological mechanisms of periprosthetic infection progress in the knee joint as well as defining new diagnostic approaches.

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Biofilm Formation by Gram-Negative Bacteria during Implant-Associated Infection

Cited by 11 publications

References 4 publications

Resistance to Antibiotics in Plankton and Biofilm Cultures of Pseudomonas aeruginosa Clinical Strains

Resistance to Antibiotics in Plankton and Biofilm Cultures of Pseudomonas aeruginosa Clinical Strains

Recent Strategies to Combat Infections from Biofilm-Forming Bacteria on Orthopaedic Implants

Immunity status in patients with periprosthetic knee joint infection

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