Biofilm formation by Escherichia coli is stimulated by synergistic interactions and co-adhesion mechanisms with adherence-proficient bacteria

Castonguay, Marie Hélène; Schaaf, Saskia van der; Koester, Wolfgang; Krooneman, Janneke; Meer, Walter van der; Harmsen, Hermie J. M.; Landini, Paolo

doi:10.1016/j.resmic.2005.10.003

Cited by 120 publications

(95 citation statements)

References 35 publications

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“…To determine the effect of insulin on E. coli's relative surface electronegativity, its adherence to glass (an electronegative substrate) was determined [29] [30]. Overnight cultures in PYNB alone, or with insulin-r (200 µU/ml) and/or glucose (0.001 g/ml) were inoculated (10 5 CFU/ml) into homologous medium with and without insulin-r and/or glucose.…”

Section: Surface Electronegativitymentioning

confidence: 99%

Effect of Human Insulin on the Formation of Catheter-Associated <i>E. coli</i> Biofilms

Plotkin¹,

Wu²,

Ward³

et al. 2014

OJU

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Biofilm formation is essential for the survival and growth of Escherichia coli in catheter-associated infections. Individuals with type 2 diabetes mellitus can excrete insulin and/or glucose in their urine. This population also has an increased incidence of urinary tract infections. The focus of this study was to determine if the composition of Foley catheter material affects biofilm formation by E. coli in a model system for type 2 diabetes mellitus. Rubber (lubricious-coated), silicon-coated, silver-coated and nitrofurazone-coated catheter segments (5 mm; n = 6) were tested. Catheter segments were added to E. coli ATCC25922 (10 4 CFU/ml, final concentration) in artificial urine alone, or with insulin (40 µU/ml) and/or glucose (0.1%). After incubation (18 h, 37˚C, in air and anaerobically) the level of catheter-associated biofilm was determined by crystal violet staining (Abs 550nm ). Statistical analysis was done by ANOVA with post-hoc analysis (Tukey). Neither nitrofurazone-coated nor silver-coated catheters supported the formation of E. coli biofilm, regardless of growth condition tested. In contrast, under aerobic biofilm formation on silicon catheters was significantly higher (p < 0.05) than that on sterile catheter alone. In addition, glucose with insulin induced significantly more biofilm (p < 0.05) than E. coli controls. Biofilm formation was also significantly increased (p < 0.05) under anaerobic conditions on lubricious-coated rubber catheters as compared to sterile catheters. These results may aid in the development of a catheter material that can prevent biofilm formation, or alternatively guide choice of catheter material for individuals shedding insulin in their urine.

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Section: Surface Electronegativitymentioning

confidence: 99%

Effect of Human Insulin on the Formation of Catheter-Associated <i>E. coli</i> Biofilms

Plotkin¹,

Wu²,

Ward³

et al. 2014

OJU

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show abstract

“…These devices are often colonized by single bacterial species like Escherichia coli [5]. E. coli is responsible for 80% of the urinary tract infections and it was observed that even after antibiotic therapy it can persist and re-emerge in the bladder and in associated urinary tract biomedical devices (eg urinary catheters) [3,6,7].…”

Section: Introductionmentioning

confidence: 99%

The effects of surface properties on Escherichia coli adhesion are modulated by shear stress

Moreira

Araújo

Miranda

et al. 2014

Colloids and Surfaces B: Biointerfaces

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The adhesion of Escherichia coli to glass and polydimethylsiloxane (PDMS) at different flow rates (between 1 and 10 ml.s -1 ) was monitored in a parallel plate flow chamber in order to understand the effect of surface properties and hydrodynamic conditions on adhesion. Computational fluid dynamics was used to assess the applicability of this flow chamber in the simulation of the hydrodynamics of relevant biomedical systems. Wall shear stresses between 0.005 and 0.07 Pa were obtained and these are similar to those found in the circulatory, reproductive and urinary systems. Results demonstrate that E.coli adhesion to hydrophobic PDMS and hydrophilic glass surfaces is modulated by shear stress with surface properties having a stronger effect at the lower and highest flow rates tested and with negligible effects at intermediate flow rates. These findings suggest that when expensive materials or coatings are selected to produce biomedical devices, this choice should take into account the physiological hydrodynamic conditions that will occur during the utilization of those devices.

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“…Indeed, temperature-dependent regulation does not take place in several pathogenic E. coli strains, in which curli are expressed even at 37 u C and appear to be involved in virulence-associated processes (Ben Nasr et al, 1996;Bian et al, 2000;Persson et al, 2003). In contrast, curli operons are silent in a large number of laboratory strains, as well as in some clinical and environmental isolates, despite the presence of functional csg genes (Romling et al, 1998a;Bokranz et al, 2005;Castonguay et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Cellulose modulates biofilm formation by counteracting curli-mediated colonization of solid surfaces in Escherichia coli

et al. 2008

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In enterobacteria, the CsgD protein activates production of two extracellular structures: thin aggregative fimbriae (curli) and cellulose. While curli fibres promote biofilm formation and cell aggregation, the evidence for a direct role of cellulose as an additional determinant for biofilm formation is not as straightforward. The MG1655 laboratory strain of Escherichia coli only produces limited amounts of curli and cellulose; however, ectopic csgD expression results in strong stimulation of curli and cellulose production. We show that, in a csgD-overexpressing derivative of MG1655, cellulose production negatively affects curli-mediated surface adhesion and cell aggregation, thus acting as a negative determinant for biofilm formation. Consistent with this observation, deletion of the bcsA gene, necessary for cellulose production, resulted in a significant increase in curli-dependent adhesion. We found that cellulose production increased tolerance to desiccation, suggesting that the function of cellulose might be related to resistance to environmental stresses rather than to biofilm formation. Production of the curli/cellulose network in enterobacteria typically takes place at low growth temperature (,32 6C), but not at 37 6C. We show that CsgD overexpression can overcome temperature-dependent control of the curliencoding csgBA operon, but not of the cellulose-related adrA gene, suggesting very tight temperature control of cellulose production in E. coli MG1655.

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Biofilm formation by Escherichia coli is stimulated by synergistic interactions and co-adhesion mechanisms with adherence-proficient bacteria

Cited by 120 publications

References 35 publications

Effect of Human Insulin on the Formation of Catheter-Associated <i>E. coli</i> Biofilms

Effect of Human Insulin on the Formation of Catheter-Associated <i>E. coli</i> Biofilms

The effects of surface properties on Escherichia coli adhesion are modulated by shear stress

Cellulose modulates biofilm formation by counteracting curli-mediated colonization of solid surfaces in Escherichia coli

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Biofilm formation by Escherichia coli is stimulated by synergistic interactions and co-adhesion mechanisms with adherence-proficient bacteria

Cited by 120 publications

References 35 publications

Effect of Human Insulin on the Formation of Catheter-Associated &lt;i&gt;E. coli&lt;/i&gt; Biofilms

Effect of Human Insulin on the Formation of Catheter-Associated &lt;i&gt;E. coli&lt;/i&gt; Biofilms

The effects of surface properties on Escherichia coli adhesion are modulated by shear stress

Cellulose modulates biofilm formation by counteracting curli-mediated colonization of solid surfaces in Escherichia coli

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Product

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Effect of Human Insulin on the Formation of Catheter-Associated <i>E. coli</i> Biofilms

Effect of Human Insulin on the Formation of Catheter-Associated <i>E. coli</i> Biofilms