Biofilm Formation and Prevalence of Biofilm-Related Genes Among Clinical Strains of Multidrug-Resistant <i>Staphylococcus aureus</i>

Kaźmierczak, Natalia; Grygorcewicz, Bartłomiej; Piechowicz, Lidia

doi:10.1089/mdr.2020.0399

Cited by 8 publications

(12 citation statements)

References 43 publications

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“…To evaluate bacteriophage antibiofilm activity, we first selected appropriate MDRSA strains. A strain was chosen for the study only when the following criteria were met: (1) it was characterized as a strong biofilm producer and (2) it was susceptible to phages vB_SauM-A, vB_SauM-C, or vB_SauM-D, which was determined in previous studies [4,12]. Phages vB_SauM-A and vB_SauM-D effectively lysed 11 biofilms producing MDRSA strains, whereas vB_SauM-C lysed only 6 out of 11 [12].…”

Section: Biofilm Eradication By Phagesmentioning

confidence: 99%

“…A total of 11 multidrug-resistant Staphylococcus aureus clinical isolates were chosen from the collection of S. aureus strains from the Department of Medical Microbiology, the Medical University of Gda ńsk. Strains were differentiated using SCCmec and spa typing and selected due to their multidrug resistance and ability to form a biofilm, which was studied previously [4,12]. All the strains tested were confirmed to be resistant to β-lactams, erythromycin, norfloxacin, and ciprofloxacin.…”

Section: Bacterial Strainsmentioning

confidence: 99%

“…A noteworthy example of a bacterium commonly causing biofilm-related infections is the opportunistic pathogen Staphylococcus aureus. Staphylococcal biofilms are associated with a broad spectrum of human diseases, such as osteomyelitis, periodontitis, endocarditis, chronic wound infection, and infection of indwelling medical devices [3,4]. Apart from increased tolerance to antibiotics of bacteria in the biofilm, more and more S. aureus strains exhibit resistance to the antibiotics previously employed.…”

Section: Introductionmentioning

confidence: 99%

“…Apart from increased tolerance to antibiotics of bacteria in the biofilm, more and more S. aureus strains exhibit resistance to the antibiotics previously employed. In addition to being resistant to βlactam antibiotics (methicillin-resistant S. aureus), most strains also become resistant to other antibiotic classes (e.g., fluoroquinolones, macrolides, aminoglycosides, and clindamycin), leading to the emergence of multidrug-resistant S. aureus (MDRSA) [4,5].…”

Section: Introductionmentioning

confidence: 99%

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Comparative Assessment of Bacteriophage and Antibiotic Activity against Multidrug-Resistant Staphylococcus aureus Biofilms

Kaźmierczak

Grygorcewicz

Roszak

et al. 2022

IJMS

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Problems connected with biofilm-related infections and antibiotic resistance necessitate the investigation and development of novel treatment strategies. Given their unique characteristics, one of the most promising alternatives to conventional antibiotics are bacteriophages. In the in vitro and in vivo larva model study, we demonstrate that phages vB_SauM-A, vB_SauM-C, and vB_SauM-D are effective antibiofilm agents. The exposure of biofilm to phages vB_SauM-A and vB_SauM-D led to 2–3 log reductions in the colony-forming unit number in most of the multidrug-resistant S. aureus strains. It was found that phage application reduced the formed biofilms independently of the used titer. Moreover, the study demonstrated that bacteriophages are more efficient in biofilm biomass removal and reduction in staphylococci count when compared to the antibiotics used. The scanning electron microscopy analysis results are in line with colony forming unit (CFU) counting but not entirely consistent with crystal violet (CV) staining. Additionally, phages vB_SauM-A, vB_SauM-C, and vB_SauM-D can significantly increase the survival rate and extend the survival time of Galleria mellonella larvae.

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Section: Biofilm Eradication By Phagesmentioning

confidence: 99%

Section: Bacterial Strainsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Assessment of Bacteriophage and Antibiotic Activity against Multidrug-Resistant Staphylococcus aureus Biofilms

Kaźmierczak

Grygorcewicz

Roszak

et al. 2022

IJMS

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“…To evaluate lactoferrin anti-biofilm activity, we selected appropriate MDRSA strains that were classified as strong biofilm producers during our previous studies [ 19 ]. Mature MDRSA biofilms treated with three different concentrations of lactoferrin: 0.1 mg/mL, 1.0 mg/mL and 10 mg/mL, which were chosen based on literature data [ 16 , 17 ].…”

Section: Resultsmentioning

confidence: 99%

Activity of Phage–Lactoferrin Mixture against Multi Drug Resistant Staphylococcus aureus Biofilms

2022

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Biofilms are complex bacterial structures composed of bacterial cells embedded in extracellular polymeric substances (EPS) consisting of polysaccharides, proteins and lipids. As a result, biofilms are difficult to eradicate using both mechanical methods, i.e., scraping, and chemical methods such as disinfectants or antibiotics. Bacteriophages are shown to be able to act as anti-biofilm agents, with the ability to penetrate through the matrix and reach the bacterial cells. However, they also seem to have their limitations. After several hours of treatment with phages, the biofilm tends to grow back and phage-resistant bacteria emerge. Therefore, it is now recommended to use a mixture of phages and other antibacterial agents in order to increase treatment efficiency. In our work we have paired staphylococcal phages with lactoferrin, a protein with proven anti-biofilm proprieties. By analyzing the biofilm biomass and metabolic activity, we have observed that the addition of lactoferrin to phage lysate accelerated the anti-biofilm effect of phages and also prevented biofilm re-growth. Therefore, this combination might have a potential use in biofilm eradication procedures in medical settings.

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Methods to Grow and Measure In Vitro Static Biofilms

Sung

Park

Chon

et al. 2022

Encyclopedia of Infection and Immunity

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Biofilm Formation and Prevalence of Biofilm-Related Genes Among Clinical Strains of Multidrug-Resistant Staphylococcus aureus

Cited by 8 publications

References 43 publications

Comparative Assessment of Bacteriophage and Antibiotic Activity against Multidrug-Resistant Staphylococcus aureus Biofilms

Comparative Assessment of Bacteriophage and Antibiotic Activity against Multidrug-Resistant Staphylococcus aureus Biofilms

Activity of Phage–Lactoferrin Mixture against Multi Drug Resistant Staphylococcus aureus Biofilms

Methods to Grow and Measure In Vitro Static Biofilms

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