Biofilm formation and presence of icaAD gene in clinical isolates of staphylococci

Nasr, Rasha A.; Abushady, Hala Mohamed; Hussein, Hussein S.

doi:10.1016/j.ejmhg.2012.04.007

Cited by 62 publications

(57 citation statements)

References 32 publications

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“…Similar to this study, several previous surveys have not exhibited significant differences between MRSA and MSSA isolates or between blood and wound isolates, but have shown that icaAD genes play important roles in PIA synthesis (31)(32)(33)(34). Similarly, in this study, icaADC genes were the predominant genes among blood and wound isolates.…”

Section: Discussionsupporting

confidence: 88%

The Comparison ofStaphylococcus aureusIsolated From Blood and Wound Specimens for Genes Encoding Polysaccharide Intercellular Adhesion (PIA)

Ghasemian¹,

Peerayeh²,

Bakhshi³

2015

Avicenna J Clin Microbiol Infect

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Background:The polysaccharide intercellular adhesion (PIA) produced by Staphylococcus aureus is effective in the protection of isolates from outer harsh conditions and progress of infection. Objectives: The aim of this study was to compare the icaABCD genes encoding polysaccharide intercellular adhesion (PIA) between blood and wound isolates of Staphylococcus aureus (S. aureus) in Tehran. Patients and Methods: Forty-eight clinical isolates (including 30 blood and 18 skin wounds) were collected from patients and were identified. Next, mecA gene, SCCmec types and icaABCD genes were detected among blood and wound isolates of S. aureus by PCR assay and specific primers. Results: Nine (19%) out of 12 methicillin resistant S. aureus (MRSA) isolates harbored SCCmec type III and three (6.2%) isolates harbored SCCmec type V. Prevalence of icaA, icaB, icaC and icaD in blood isolates was twenty-one (70%), fourteen (48%), nineteen (64%) and eighteen (60%), respectively; while the prevalence in wound isolates was as nine (50%), seven (39%), six (34%) and twelve (67%), respectively. Conclusions: These findings showed no significant difference regarding the presence of icaADBC genes between blood and wound isolates.

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Section: Discussionsupporting

confidence: 88%

The Comparison ofStaphylococcus aureusIsolated From Blood and Wound Specimens for Genes Encoding Polysaccharide Intercellular Adhesion (PIA)

Ghasemian¹,

Peerayeh²,

Bakhshi³

2015

Avicenna J Clin Microbiol Infect

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“…These variations in frequency of biofilm producing staphylococci are documented in other parts of the world, as well. The rates of biofilm production in staphylococci isolates have been reported in the range of approximately 30% to more than 70% in different Asian, African, and European countries (20,25,26).…”

Section: Discussionmentioning

confidence: 99%

Detection of Biofilm Production Capability and icaA/D Genes Among Staphylococci Isolates from Shiraz, Iran

Zalipour

Ebrahim‐Saraie

Sarvari

et al. 2016

Jundishapur J Microbiol

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Background: Biofilm formation capacity is recognized as an important virulence factor in staphylococci that makes the organisms more resistant to antibiotics and host defenses. Objectives: This study aimed to determine the biofilm producing ability and presence of icaA/D genes in staphylococcal isolates obtained from different clinical specimens. Methods: This cross-sectional study was performed on a total of 151 staphylococcal isolates (79 Staphylococcus aureus and 72 S. epidermidis) obtained from different clinical specimens from February to August 2013 in Shiraz, Southwest of Iran. Slime production ability was evaluated using the both phenotypic (by cultivation of staphylococcal isolates on Congo red agar (CRA)) and genotypic (detection of the presence of icaA/D genes by PCR) methods. Results: Overall, of the 79 S. aureus isolates tested with CRA method, 64.7% of methicillin-resistant S. aureus (MRSA) isolates, and 46.7% of methicillin-sensitive S. aureus (MSSA) isolates were able to produce biofilm. The relative frequency of biofilm producing S. epidermidis isolates was 70.8% that was significantly higher than that of S. aureus isolates. The most common source of biofilm producing isolates in both S. aureus and S. epidermidis isolates was endotracheal tube (ETT) with 100% biofilm formation. Moreover, the presence of icaA/D genes was detected in 63.3% and 81.9% of S. aureus and S. epidermidis isolates, respectively. Conclusions: The remarkable rates of biofilm production ability among clinically isolated staphylococci emphasize the necessity of more effective infections control policies to prevent biofilm formation on medical devices and hospital environmental surfaces.

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“…For instance, Nasr et al (34) detected icaAD genes in 32% of blood and catheter isolates. In the other study, 36 of 46 Staphylococcal isolates harbored icaA and icaD genes; while Grinholc and coworkers did not detect icaD, but all strains were icaA positive (35).…”

Section: Discussionmentioning

confidence: 99%

High Prevalence of Icaadbc Genes Responsible for Biofilm Formation in Clinical Isolates of Staphylococcus aureus From Hospitalized Children

Ghasemian

Peerayeh

Bakhshi

et al. 2015

Arch Pediatr Infect Dis

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Background:The icaABCD genes encode a Polysaccharide Intercellular Adhesion (PIA), which is a tight structure protecting Staphylococcus aureus community against adverse environmental conditions. The ica dependent biofilm formation plays an important role in persistent infections in hospitalized patients. Objectives: The aim of this study was to detect icaADBC genes encoding PIA among S. aureus isolates from children in Loghman Hospital of Tehran. Materials and Methods: We collected 22 clinical specimens from hospitalized pediatrics and identified the isolates. Then, we detected mecA gene among Methicillin Resistant S. aureus (MRSA), SCCmec types and icaABCD gens by PCR assay and specific primers. Results: Five isolates (22.7%) were methicillin resistant (MRSA) and mecA gene was detected among them. All the MRSA isolates harbored SCCmec type III. Prevalence of icaA, icaB, icaC and icaD in the isolates were 16 (73%), 14 (63.6%), 16 (73%) and 16 (73%), respectively. Moreover, all the MRSA strains were icaADBC positive. Conclusions: Prevalence of icaADBC genes was relatively high among children and also all the four ica genes were detected among MRSA strains.

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Biofilm formation and presence of icaAD gene in clinical isolates of staphylococci

Cited by 62 publications

References 32 publications

The Comparison ofStaphylococcus aureusIsolated From Blood and Wound Specimens for Genes Encoding Polysaccharide Intercellular Adhesion (PIA)

The Comparison ofStaphylococcus aureusIsolated From Blood and Wound Specimens for Genes Encoding Polysaccharide Intercellular Adhesion (PIA)

Detection of Biofilm Production Capability and icaA/D Genes Among Staphylococci Isolates from Shiraz, Iran

High Prevalence of Icaadbc Genes Responsible for Biofilm Formation in Clinical Isolates of Staphylococcus aureus From Hospitalized Children

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