Biofilm Formation and Associated Gene Expression in Multidrug-Resistant Klebsiella pneumoniae Isolated from Clinical Specimens

Ashwath, Priyanka; Deekshit, Vijaya Kumar; Rohit, Anusha; Dhinakaran, I; Karunasagar, Iddya; Karunasagar, Indrani; Akhila, Dharnappa Sannejal

doi:10.1007/s00284-022-02766-z

Cited by 18 publications

(23 citation statements)

References 57 publications

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“…The copyright holder for this preprint this version posted September 7, 2022. ; https://doi.org/10.1101/2022.09.07.506922 doi: bioRxiv preprint amikacin, gentamicin, ciprofloxacin, cefazolin, cefoperazone sulbactam, ceftriaxone, cefepime, cefotaxime, and cefuroxime. Reports suggest that the presence of plasmids carrying resistant genes in K. pneumoniae, modulates the expression of diverse biofilm associated genes (Ashwath et al, 2022). Possibly this may be true for the multiple drug resistant K. pneumoniae isolated from clinics in our study.…”

Section: Discussionmentioning

confidence: 67%

“…In our study also we observed 25% colistin resistant isolates, which is certainly concerning. The increased antibiotic resistance is attributed to several factors, however, the prevalence of ESBL encoding genes such as bla SHV , bla CTX-M , bla TEM ; and plasmids harboring quinolone resistant qnrS, qnrA, qnrB , and colistin resistant mcr genes, are regarded as the main contributor of resistance among K. pneumoniae (Piperaki et al, 2017; Ashwath et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…K. pneumoniae 100% resistance was observed against co-trimoxazole, 85% resistance was observed against imipenem, meropenem, tigecycline, and ofloxacin, and 71% resistance was observed against amoxicillin clavulanate, amikacin, gentamicin, ciprofloxacin, cefazolin, cefoperazone sulbactam, ceftriaxone, cefepime, cefotaxime, and cefuroxime. Reports suggest that the presence of plasmids carrying resistant genes in K. pneumoniae , modulates the expression of diverse biofilm associated genes (Ashwath et al, 2022). Possibly this may be true for the multiple drug resistant K. pneumoniae isolated from clinics in our study.…”

Section: Discussionmentioning

confidence: 99%

“…Besides, K. pneumoniae isolates also showed the least sensitivity toward other carbapenems, viz. meropenem and ertapenem (Papadimitriou-Olivgeris et al,2017; Matovina et al, 2021., Ashwath et al, 2022)…”

Section: Discussionmentioning

confidence: 99%

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Successfully treating biofilms of extensively drug resistant Klebsiella pneumoniae isolates from hospital patients with N-Acetyl Cysteine

Chauhan

Bhowmik

Rohit

2022

Preprint

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Klebsiella pneumoniae is one of the leading causes of community and nosocomial infections. Reduced treatment options against extensively drug resistant (XDR) - K. pneumoniae, is a serious concern in hospital settings, and hence, WHO has categorized it as a critical priority pathogen. Biofilm forming ability is a common virulence mechanism amongst K. pneumoniae that is associated with antibiotic tolerance up to 1000X MIC and hence, are difficult to treat. N-acetyl cysteine (NAC) is an FDA approved mucolytic drug used to treat acetaminophen-associated toxicity and obstructive pulmonary diseases. In this study, we assessed antibacterial and antibiofilm activity of NAC against clinical isolates of XDR K. pneumoniae, obtained from Madras Medical Mission Hospital, India. To assess the biofilm eradication ability of NAC, we grew biofilms in 96 well plates and treated the mature biofilms with different concentrations of NAC. We observed that the biofilms of only 3 isolates of XDR K. pneumoniae could be eradicated at a concentration as low as 20mg/ml. Although increasing the concentration of NAC to 80mg/mL could significantly reduce the biofilms of all the isolates up to 4-5 Log, NAC at a concentration of 100 mg/mL successfully eradicated the mature biofilms of all the isolates of XDR K. pneumoniae. This in vitro study demonstrates the potential of NAC as an efficient agent against the biofilms of clinical isolates of XDR-K. pneumoniae and thus provides a promising alternative to antibiotics.

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Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Successfully treating biofilms of extensively drug resistant Klebsiella pneumoniae isolates from hospital patients with N-Acetyl Cysteine

Chauhan

Bhowmik

Rohit

2022

Preprint

Self Cite

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“…35 Advances in microbiology have developed molecular detection of biofilm-associated genes using polymerase chain reaction (PCR)-based techniques such as qualitative real-time PCR, multiplex PCR, conventional PCR, and in-silico PCR are used for the identification of pathogens by amplifying species-specific nucleic acid sequences and virulence factors by amplifying target virulence genes such as biofilm genes with the usage of gene-specific primers. [36][37][38]…”

Section: Laboratory Diagnosis Of Bacterial Biofilmsmentioning

confidence: 99%

Biofilm-Associated Multi-Drug Resistance in Hospital-Acquired Infections: A Review

Assefa¹,

Amare²

2022

IDR

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Biofilm-related multi-drug resistance (MDR) is a major problem in hospital-acquired infections (HAIs) that increase patient morbidity and mortality rates and economic burdens such as high healthcare costs and prolonged hospital stay. This review focuses on the burden of bacterial biofilm in the hospital settings, their impact on the emergence of MDR in the HAIs, biofilm detection methods, recent approaches against biofilms, and future perspectives. The prevalence of biofilm-associated MDR among HAIs ranges from 17.9% to 100.0% worldwide. The predominant bacterial isolates causing HAIs in recently published studies were S. aureus , A. baumannii, K. pneumoniae , and P. aeruginosa . In addition to the use of qualitative and quantitative methods to detect biofilm formation, advanced PCR-based techniques have been performed for detecting biofilm-associated genes. Although there are suggested therapeutic strategies against biofilms, further confirmation of their efficacy for in vivo application and antibiotics targeting biofilm-associated genes/proteins to minimize treatment failure is required for the future.

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Sequence-Specific Gene Silencing of acrA in the Multi-drug Efflux System AcrAB Induces Sensitivity in Drug-Resistant Klebsiella pneumoniae

et al. 2022

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Biofilm Formation and Associated Gene Expression in Multidrug-Resistant Klebsiella pneumoniae Isolated from Clinical Specimens

Cited by 18 publications

References 57 publications

Successfully treating biofilms of extensively drug resistant Klebsiella pneumoniae isolates from hospital patients with N-Acetyl Cysteine

Successfully treating biofilms of extensively drug resistant Klebsiella pneumoniae isolates from hospital patients with N-Acetyl Cysteine

Biofilm-Associated Multi-Drug Resistance in Hospital-Acquired Infections: A Review

Sequence-Specific Gene Silencing of acrA in the Multi-drug Efflux System AcrAB Induces Sensitivity in Drug-Resistant Klebsiella pneumoniae

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