Biofilm formation and antimicrobial susceptibility Pattern of Methicillin Resistant Staphylococcus aureus from wound infection

Kaur, Dardi Charan; Wankhede, S. V.

doi:10.21276/apjhs.2014.1.4.5

Cited by 10 publications

(9 citation statements)

References 21 publications

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“…One of the main reasons of antibiotic resistance is the rebellious nature of biofilms produced by these pathogens. In this study, 85/116 (73%) of isolates were identified as biofilm producers, that is compatible with other studies investigated biofilm production by S. aureus isolated from wound samples [ 43 , 44 ]. Our study focused on the development of promising alternative agents for treatment of these serious infections such as ZnO-NPs.…”

Section: Discussionsupporting

confidence: 89%

Effect of ZnO nanoparticles on methicillin, vancomycin, linezolid resistance and biofilm formation in Staphylococcus aureus isolates

Abdelraheem

Khairy

Zaki

et al. 2021

Ann Clin Microbiol Antimicrob

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Background Multidrug resistant (MDR) and biofilm producing Staphylococcus aureus strains are usually associated with serious infections. This study aimed to evaluate the antibacterial and antibiofilm-formation effects of zinc oxide nanoparticles (ZnO-NPs) against staphylococcus aureus (S. aureus) isolates. Methods A total of 116 S. aureus isolates were recovered from 250 burn wound samples. The antimicrobial/antibiofilm effects of ZnO-NPs against methicillin, vancomycin and linezolid resistant S. aureus (MRSA, VRSA and LRSA) isolates were examined using phenotypic and genotypic methods. The minimum inhibitory concentration (MIC) of ZnO-NPs was determined by microdilution method. The effects of sub-MIC concentrations of ZnO-NPs on biofilm formation and drug resistance in S. aureus were determined by the microtiter plate method. The change in the expression levels of the biofilm encoding genes and resistance genes in S. aureus isolates after treatment with ZnO-NPs was assessed by real time reverse transcriptase PCR (rt-PCR). Results MICs of ZnO-NPs in S. aureus isolates were (128–2048 µg/ml). The sub-MIC of ZnO-NPs significantly reduced biofilm formation rate (the highest inhibition rate was 76.47% at 1024 µg/ml) and the expression levels of biofilm genes (ica A, ica D and fnb A) with P < 0.001. Moreover, Sub-MIC of ZnO-NPs significantly reduced the rates of MRSA from 81.9 (95 isolates) to 13.30% (15 isolates), VRSA from 33.60 (39 isolates) to 0% and LARSA from 29.30 (34) to 0% as well as the expression levels of resistance genes (mec A, van A and cfr) with P value < 0.001. Conclusion ZnO-NPs can be used as antibiofilm and potent antimicrobial against MRSA, VRSA and LRSA isolates.

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Section: Discussionsupporting

confidence: 89%

Effect of ZnO nanoparticles on methicillin, vancomycin, linezolid resistance and biofilm formation in Staphylococcus aureus isolates

Abdelraheem

Khairy

Zaki

et al. 2021

Ann Clin Microbiol Antimicrob

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“…The prevalence of MRSA in various hospitals in the world ranges from 2-70% with an average rate of 20% (Yuwono & Biomed, 2010) and causes major risk and also potentially causes significant mortality and morbidity rates in human populations because of the ability to form a biofilm. Biofilm have an enormous impact on healthcare and are estimated to be associated with 65% of nosocomial infections (Kaur & Wankhede, 2014).…”

Section: Resultsmentioning

confidence: 99%

“…Several infections caused by gram-positive bacteria, including those are caused by S.epidermidis, S. aureus and Enterococci are proved very difficult to treat with partial antibiotic therapy due to their very high natural resistance levels and partly because they form a biofilm (O'Toole et al, 2000). Biofilm formation of S.aureus is managed by PIA expression which mediates the adherents between cell to cell and it is a product from a gene of ica ABCD (Kaur & Wankhede, 2014).…”

Section: Resultsmentioning

confidence: 99%

“…MRSA creates a large risk and has the potential to cause significant mortality and morbidity in the human population because it is able to form a biofilm. Biofilm has a considerable impact on health and are estimated to be associated with 65% of nosocomial infections (Kaur & Wankhede, 2014). Riemann and Oliver (2006), defines the biofilm as a collection of microorganisms and associated extracellular products on its surface and commonly attached to biological and non-biological substrates.…”

mentioning

confidence: 99%

“…The formation of biofilm in S. aureus is governed by PIA expression mediating cell-to-cell attachment and is the product of the genes of ABDC ica (Kaur & Wankhede, 2014). The product of the ica A gene is a homologous transmembrane protein with N-acetylglucosaminyltransferase, which requires the ica D gene product for its optimum activity.…”

mentioning

confidence: 99%

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Biofilm formation and detection of A/D genes in MRSA (Methicillin-Resistant Staphylococcus aureus) and MSSA (Methicillin-Sensitive Staphylococcus aureus)

Purbowati

2019

Berkala Penelitian Hayati

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MRSA (Methicillin-Resistant Staphylococcus aureus) arises due to excessive use of methicillin antibiotics and complications from all medical practices that have been carried out lately. The prevalence of MRSA tends to increase and its ability to form biofilms so that it has the potential to significantly increase mortality and morbidity. Biofilms are considered one of the microbial virulence factors. Biofilm formation in S. aureus is regulated by the expression of PIA which mediates attachment between cells to cells and is a product of the ABDC ica gene. This study aims to determine the ability of biofilm formation as one of the virulence factors in MRSA compared to MSSA (Methicillin-Sensitive Staphylococcus aureus). its relationship to the presence of A and D ica genes as biofilm coding genes. Biofilm formation assay on MRSA and MSSA using the Microtiter Plate (MtP) method and the A/D gene detection assay performed by PCR method. The results showed that the incubation end value of Optical Density (OD) for MSSA1 (0.75), MSSA2 (0.46), MRSA1 (0.53) isolates according to the formula were interpreted as moderate biofilm producer. The conclusions of this study were all of the test isolates (MSSA1, MSSA2 and MRSA1) were able to form a biofilm. Distribution of ica A and ica D genes in MSSA 1 and MSSA 2 was varied while MRSA1 had the ica A and ica D genes.

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A multi-enzyme cascade microneedle reaction system for hierarchically MRSA biofilm elimination and diabetic wound healing

Zhao

et al. 2023

Chemical Engineering Journal

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Biofilm formation and antimicrobial susceptibility Pattern of Methicillin Resistant Staphylococcus aureus from wound infection

Cited by 10 publications

References 21 publications

Effect of ZnO nanoparticles on methicillin, vancomycin, linezolid resistance and biofilm formation in Staphylococcus aureus isolates

Effect of ZnO nanoparticles on methicillin, vancomycin, linezolid resistance and biofilm formation in Staphylococcus aureus isolates

Biofilm formation and detection of A/D genes in MRSA (Methicillin-Resistant Staphylococcus aureus) and MSSA (Methicillin-Sensitive Staphylococcus aureus)

A multi-enzyme cascade microneedle reaction system for hierarchically MRSA biofilm elimination and diabetic wound healing

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